Incorporation of [14C]glucose into cell wall polysaccharides during Ags1 depletion either in the absence or presence of 1.2 M sorbitol
| % Incorporation of [14C]glucoseb | ||||||
| Growth | Strain | Thiaminea | Cell wall | α-Glucan | β-Glucan | Galactomannan |
| EMM | Control | − (on, wt) | 28.1 ± 2.8 (100)c | 5.5 ± 0.3 (19.8) | 19.2 ± 1.6 (68.5) | 3.3 ± 0.8 (11.7) |
| EMM | 81X-ags1+ | + (off) | 21.2 ± 0.7 (100) | 2.6 ± 0.1 (12.1) | 16.5 ± 1.2 (77.8) | 2.1 ± 0.6 (10.1) |
| EMM+S | Control | − (on, wt) | 26.8 ± 3.0 (100) | 5.3 ± 1.0 (19.9) | 17.9 ± 1.3 (66.9) | 3.6 ± 0.8 (13.2) |
| EMM+S | 81X-ags1+ | + (off) | 32.0 ± 2.7 (100) | 3.4 ± 0.6 (10.9) | 25.0 ± 3.2 (77.7) | 3.6 ± 0.5 (11.4) |
| % Incorporation of [14C]glucoseb | ||||||
| Growth | Strain | Thiaminea | Cell wall | α-Glucan | β-Glucan | Galactomannan |
| EMM | Control | − (on, wt) | 28.1 ± 2.8 (100)c | 5.5 ± 0.3 (19.8) | 19.2 ± 1.6 (68.5) | 3.3 ± 0.8 (11.7) |
| EMM | 81X-ags1+ | + (off) | 21.2 ± 0.7 (100) | 2.6 ± 0.1 (12.1) | 16.5 ± 1.2 (77.8) | 2.1 ± 0.6 (10.1) |
| EMM+S | Control | − (on, wt) | 26.8 ± 3.0 (100) | 5.3 ± 1.0 (19.9) | 17.9 ± 1.3 (66.9) | 3.6 ± 0.8 (13.2) |
| EMM+S | 81X-ags1+ | + (off) | 32.0 ± 2.7 (100) | 3.4 ± 0.6 (10.9) | 25.0 ± 3.2 (77.7) | 3.6 ± 0.5 (11.4) |
ags1+-repressed cell cultures were grown for 3 (EMM) or 7 h (EMM+S) in the presence of thiamine. [14C]glucose was added 1.5 or 4 h before harvesting, respectively. S, 1.2 M sorbitol.
Percent incorporation of [14C]glucose = cpm incorporated per fraction × 100/total cpm incorporated. Values are the means and SDs calculated from three independent experiments.
Values are percentages of the corresponding polysaccharide in the cell. Values in parentheses are percentages of the polysaccharide in the cell wall.