Table 1.

Viability of num1 alleles in combination with kar9Δ mutant

Mutant crossed with kar9Δ No. of tetrads analyzed No. of predicted double mutants Viability of double mutant 
   Microcolony Viable 
num1Δ 11 12 12 − 
NUM1-GFP 12 12 − 12 
ΔCC-GFP 14 14 14 − 
ΔCC1-GFP 14 10 10 − 
ΔTR-GFP 13 12 − 12 
CC1–303-PH-GFP 16 20 − 20 
CC1–303-GFP 12 13 13 − 
CC1–303-GFP-CAAX 21 23 − 23 
num1L167E + L170E 12 13 13 − 
Mutant crossed with kar9Δ No. of tetrads analyzed No. of predicted double mutants Viability of double mutant 
   Microcolony Viable 
num1Δ 11 12 12 − 
NUM1-GFP 12 12 − 12 
ΔCC-GFP 14 14 14 − 
ΔCC1-GFP 14 10 10 − 
ΔTR-GFP 13 12 − 12 
CC1–303-PH-GFP 16 20 − 20 
CC1–303-GFP 12 13 13 − 
CC1–303-GFP-CAAX 21 23 − 23 
num1L167E + L170E 12 13 13 − 

Indicated strains were crossed with kar9Δ. The resulting diploid strains were sporulated, and tetrads were dissected.

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