Viability of num1 alleles in combination with kar9Δ mutant
| Mutant crossed with kar9Δ | No. of tetrads analyzed | No. of predicted double mutants | Viability of double mutant | |
| Microcolony | Viable | |||
| num1Δ | 11 | 12 | 12 | − |
| NUM1-GFP | 12 | 12 | − | 12 |
| ΔCC-GFP | 14 | 14 | 14 | − |
| ΔCC1-GFP | 14 | 10 | 10 | − |
| ΔTR-GFP | 13 | 12 | − | 12 |
| CC1–303-PH-GFP | 16 | 20 | − | 20 |
| CC1–303-GFP | 12 | 13 | 13 | − |
| CC1–303-GFP-CAAX | 21 | 23 | − | 23 |
| num1L167E + L170E | 12 | 13 | 13 | − |
| Mutant crossed with kar9Δ | No. of tetrads analyzed | No. of predicted double mutants | Viability of double mutant | |
| Microcolony | Viable | |||
| num1Δ | 11 | 12 | 12 | − |
| NUM1-GFP | 12 | 12 | − | 12 |
| ΔCC-GFP | 14 | 14 | 14 | − |
| ΔCC1-GFP | 14 | 10 | 10 | − |
| ΔTR-GFP | 13 | 12 | − | 12 |
| CC1–303-PH-GFP | 16 | 20 | − | 20 |
| CC1–303-GFP | 12 | 13 | 13 | − |
| CC1–303-GFP-CAAX | 21 | 23 | − | 23 |
| num1L167E + L170E | 12 | 13 | 13 | − |
Indicated strains were crossed with kar9Δ. The resulting diploid strains were sporulated, and tetrads were dissected.