Table III. Summary of properties of WT... | Rockefeller University Press

Table III.

Summary of properties of WT and mutant She2p constructs

She2p	Oligomeric state in solution	Binds to She3p in vitro	Processive	Binds to mRNA in vitro	Correct localization of ASH1 mRNA in yeast
WT*	Tetramer	++++	+	++++^a	+
				K_d = 0.1 µM^b
S120Y	Dimer	+	−	+^a	−
L130Y	Dimer	+++	−	++^a	+
				K_d = 1.0 µM^b
Δhelix	Tetramer	−	−	ND	−

She2p	Oligomeric state in solution	Binds to She3p in vitro	Processive	Binds to mRNA in vitro	Correct localization of ASH1 mRNA in yeast
WT*	Tetramer	++++	+	++++a	+
				K_d = 0.1 µMb
S120Y	Dimer	+	−	+a	−
L130Y	Dimer	+++	−	++a	+
				K_d = 1.0 µMb
Δhelix	Tetramer	−	−	ND	−

Relative ability of 1 µM She2p to bind to mRNA using filter-binding experiments (Niessing et al., 2004).

Values from mRNA filter-binding experiments using the ASH1 mRNA E3 zip code element (Müller et al., 2009).