Table I. Cofilin mutants used in this... | Rockefeller University Press

Table I.

Cofilin mutants used in this study

Strain	Corresponding S. cerevisiae strain (point mutations)	Amino acid substitutions	Growth at 25°C	Growth at 36°C	Synthetic lethal with Δaip1	Synthetic lethal with ts tropomyosin mutant cdc8-27	Synthetic interaction with α-actinin deletion mutant
adf1-1	None	L57S	Normal	None	Yes	No	N/T
adf1-M1	cof1-5 (D10A E11A)	P10A, E11A	Normal	Normal	Yes	N/T	N/T
adf1-M1A	None	E11A	Normal	Normal	N/T	N/T	N/T
adf1-M2	cof1-8 (K23A, K24A, K26A)	K23A, S24A, R26A	Slow	Slow	Yes	No	Synthetic slow growth
adf1-M2A	None	K23A	Slow	Slow	N/T	No	N/T
adf1-M2B	None	K23A, R26A	Slow	Slow	N/T	N/T	N/T
adf1-M3	None	E132A, K133A	Slow	Slow	Yes	No	N/T
adf1-M3A	cof1-22 (E134A, R135A, R138A)	E132A, K133A, R136A	None	None			N/T

Strain	Corresponding S. cerevisiae strain (point mutations)	Amino acid substitutions	Growth at 25°C	Growth at 36°C	Synthetic lethal with Δaip1	Synthetic lethal with ts tropomyosin mutant cdc8-27	Synthetic interaction with α-actinin deletion mutant
adf1-1	None	L57S	Normal	None	Yes	No	N/T
adf1-M1	cof1-5 (D10A E11A)	P10A, E11A	Normal	Normal	Yes	N/T	N/T
adf1-M1A	None	E11A	Normal	Normal	N/T	N/T	N/T
adf1-M2	cof1-8 (K23A, K24A, K26A)	K23A, S24A, R26A	Slow	Slow	Yes	No	Synthetic slow growth
adf1-M2A	None	K23A	Slow	Slow	N/T	No	N/T
adf1-M2B	None	K23A, R26A	Slow	Slow	N/T	N/T	N/T
adf1-M3	None	E132A, K133A	Slow	Slow	Yes	No	N/T
adf1-M3A	cof1-22 (E134A, R135A, R138A)	E132A, K133A, R136A	None	None			N/T

Growth was measured on YE5s agar plates as in Fig. 1. N/T, not tested.