Kinetic parameters of centrosomal microtubule polymerization dynamics in mitosis
| Parameters | RNAi | ||
| Control | Sentin | Kinesin-13Klp10A | |
| Growth rate (µm/min) | 10.8 ± 1.6 | 8.8 ± 2.4 | 9.8 ± 1.3 |
| 10.8 ± 2.1m | 8.7 ± 4.0m | 9.7 ± 2.5m | |
| Shrink rate (µm/min) | 19.6 ± 4.4 | 21.6 ± 4.9 | 40.2 ± 8.7 |
| 19.5 ± 5.2m | 21.7 ± 6.5m | 40.2 ± 12.0m | |
| Catastrophe frequency (s−1) | 0.035 ± 0.006 | 0.021 ± 0.008 | 0.025 ± 0.007 |
| Time in growth (%) | 53.1 ± 8.6 | 34.7 ± 15.3 | 62.7 ± 8.4 |
| Time in shrink (%) | 32.0 ± 7.5 | 17.8 ± 7.3 | 18.9 ± 6.0 |
| Time in pause (%) | 14.9 ± 11.1 | 47.5 ± 17.7 | 18.4 ± 10.6 |
| Parameters | RNAi | ||
| Control | Sentin | Kinesin-13Klp10A | |
| Growth rate (µm/min) | 10.8 ± 1.6 | 8.8 ± 2.4 | 9.8 ± 1.3 |
| 10.8 ± 2.1m | 8.7 ± 4.0m | 9.7 ± 2.5m | |
| Shrink rate (µm/min) | 19.6 ± 4.4 | 21.6 ± 4.9 | 40.2 ± 8.7 |
| 19.5 ± 5.2m | 21.7 ± 6.5m | 40.2 ± 12.0m | |
| Catastrophe frequency (s−1) | 0.035 ± 0.006 | 0.021 ± 0.008 | 0.025 ± 0.007 |
| Time in growth (%) | 53.1 ± 8.6 | 34.7 ± 15.3 | 62.7 ± 8.4 |
| Time in shrink (%) | 32.0 ± 7.5 | 17.8 ± 7.3 | 18.9 ± 6.0 |
| Time in pause (%) | 14.9 ± 11.1 | 47.5 ± 17.7 | 18.4 ± 10.6 |
Means ± SD. SD represents either microtubule to microtubule (marked with an m) or cell to cell (others) variability (20 microtubules and 10 cells).
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