Order properties of septin structures analyzed by polarized fluorescence
| Strain | Type | Offset | Pop var | Ring var | polRatio | n |
| Ag Cdc12-conGFP4 | ir | 87° | 0.04 | 0.14 | 1.95 ± 0.47 | 13 |
| Ag Cdc12-conGFP4 | split | 180° | 0.17 | 0.34 | 1.43 ± 0.18 | 10 |
| Ag Cdc12-conGFP0 | ir | 86° | 0.07 | 0.3 | 1.52 ± 0.18 | 11 |
| Ag Cdc12-conGFP0 | split | 4° | 0.07 | 0.41 | 1.27 ± 0.14 | 10 |
| Ag Cdc12-conGFP3 | ir | 0° | 0.15 | 0.27 | 1.41 ± 0.16 | 12 |
| Ag Cdc12-conGFP3 | split | 88° | 0.18 | 0.48 | 1.24 ± 0.10 | 10 |
| Ag Cdc3-conGFP | ir | 86° | 0.06 | 0.1 | 1.90 ± 0.71 | 11 |
| Ag Cdc3-conGFP | split | 180° | 0.11 | 0.24 | 1.33 ± 0.18 | 10 |
| Sc Cdc12-conGFP4 | hg | 92° | 0.02 | 0.03 | 1.97 ± 0.51 | 13 |
| Sc Cdc12-conGFP4 | split | 3° | 0.01 | 0.15 | 1.74 ± 0.23 | 10 |
| Sc Cdc12-conGFP0 | hg | 96° | 0.07 | 0.1 | 1.39 ± 0.16 | 11 |
| Sc Cdc12-conGFP0 | split | 1° | 0.02 | 0.17 | 1.49 ± 0.29 | 10 |
| Sc Cdc12-conGFP3 | hg | 175° | 0.06 | 0.07 | 1.53 ± 0.18 | 10 |
| Sc Cdc12-conGFP3 | split | 96° | 0.05 | 0.22 | 1.32 ± 0.10 | 10 |
| Sc Cdc3-conGFP | hg | 93° | 0.03 | 0.06 | 1.51 ± 0.10 | 10 |
| Sc Cdc3-conGFP | split | 2° | 0.02 | 0.11 | 1.82 ± 0.35 | 10 |
| MDCK SEPT2 1-344 | fibers | 89° | 0.05 | 0.12 | 1.21 ± 0.08 | 10 |
| MDCK SEPT2 1-340 | fibers | 90° | 0.02 | 0.06 | 1.27 ± 0.14 | 10 |
| MDCK SEPT2 | fibers | 92° | 0.04 | 0.18 | 1.17 ± 0.06 | 10 |
| Ag Cdc12-conGFP4 | FCF | 91° | 0.01 | 0.01 | 3.91 ± 1.39 | 10 |
| Ag Cdc3-conGFP | fibers | 60° | 0.01 | 0.04 | 1.44 ± 0.32 | 11 |
| Ag Cdc3-conGFP | fibers | 122° | 0.03 | 0.04 | 1.69 ± 0.33 | 14 |
| Strain | Type | Offset | Pop var | Ring var | polRatio | n |
| Ag Cdc12-conGFP4 | ir | 87° | 0.04 | 0.14 | 1.95 ± 0.47 | 13 |
| Ag Cdc12-conGFP4 | split | 180° | 0.17 | 0.34 | 1.43 ± 0.18 | 10 |
| Ag Cdc12-conGFP0 | ir | 86° | 0.07 | 0.3 | 1.52 ± 0.18 | 11 |
| Ag Cdc12-conGFP0 | split | 4° | 0.07 | 0.41 | 1.27 ± 0.14 | 10 |
| Ag Cdc12-conGFP3 | ir | 0° | 0.15 | 0.27 | 1.41 ± 0.16 | 12 |
| Ag Cdc12-conGFP3 | split | 88° | 0.18 | 0.48 | 1.24 ± 0.10 | 10 |
| Ag Cdc3-conGFP | ir | 86° | 0.06 | 0.1 | 1.90 ± 0.71 | 11 |
| Ag Cdc3-conGFP | split | 180° | 0.11 | 0.24 | 1.33 ± 0.18 | 10 |
| Sc Cdc12-conGFP4 | hg | 92° | 0.02 | 0.03 | 1.97 ± 0.51 | 13 |
| Sc Cdc12-conGFP4 | split | 3° | 0.01 | 0.15 | 1.74 ± 0.23 | 10 |
| Sc Cdc12-conGFP0 | hg | 96° | 0.07 | 0.1 | 1.39 ± 0.16 | 11 |
| Sc Cdc12-conGFP0 | split | 1° | 0.02 | 0.17 | 1.49 ± 0.29 | 10 |
| Sc Cdc12-conGFP3 | hg | 175° | 0.06 | 0.07 | 1.53 ± 0.18 | 10 |
| Sc Cdc12-conGFP3 | split | 96° | 0.05 | 0.22 | 1.32 ± 0.10 | 10 |
| Sc Cdc3-conGFP | hg | 93° | 0.03 | 0.06 | 1.51 ± 0.10 | 10 |
| Sc Cdc3-conGFP | split | 2° | 0.02 | 0.11 | 1.82 ± 0.35 | 10 |
| MDCK SEPT2 1-344 | fibers | 89° | 0.05 | 0.12 | 1.21 ± 0.08 | 10 |
| MDCK SEPT2 1-340 | fibers | 90° | 0.02 | 0.06 | 1.27 ± 0.14 | 10 |
| MDCK SEPT2 | fibers | 92° | 0.04 | 0.18 | 1.17 ± 0.06 | 10 |
| Ag Cdc12-conGFP4 | FCF | 91° | 0.01 | 0.01 | 3.91 ± 1.39 | 10 |
| Ag Cdc3-conGFP | fibers | 60° | 0.01 | 0.04 | 1.44 ± 0.32 | 11 |
| Ag Cdc3-conGFP | fibers | 122° | 0.03 | 0.04 | 1.69 ± 0.33 | 14 |
Ir, “inter-region” septin rings formed along the hyphae of A. gossypii, made of discrete bars (Fig. 1); hg, “hourglass” septin rings in S. cerevisiae, appear contiguous by light microscopy; split, hg or ir rings which have undergone septation; FCF/fibers, septins organized into fibrous structures, “FCF” fibers induced by treatment with FCF; offset, angle between polarized fluorescence and ring axis, averaged over the population of rings analyzed; pop var, “population variance” in the average dipole orientation of the population of rings analyzed. For pop var and ring var, scale = 0–1; 0 = no variance; 1 = complete variance/randomness. ring var, “ring variance” denotes the average variability of dipole coherence across a single structure; each individual ring var was found using weighted averaging, “vars” were arithmetically averaged for final “ring var”. For pop var and ring var, scale = 0–1; 0 = no variance; 1 = complete variance/randomness. polRatio, “polarization ratio” denotes the average strength of order for septin structures; 1 = not ordered.