Table I.

Order properties of septin structures analyzed by polarized fluorescence

Strain Type Offset Pop var Ring var polRatio n 
Ag Cdc12-conGFP4 ir 87° 0.04 0.14 1.95 ± 0.47 13 
Ag Cdc12-conGFP4 split 180° 0.17 0.34 1.43 ± 0.18 10 
Ag Cdc12-conGFP0 ir 86° 0.07 0.3 1.52 ± 0.18 11 
Ag Cdc12-conGFP0 split 4° 0.07 0.41 1.27 ± 0.14 10 
Ag Cdc12-conGFP3 ir 0° 0.15 0.27 1.41 ± 0.16 12 
Ag Cdc12-conGFP3 split 88° 0.18 0.48 1.24 ± 0.10 10 
Ag Cdc3-conGFP ir 86° 0.06 0.1 1.90 ± 0.71 11 
Ag Cdc3-conGFP split 180° 0.11 0.24 1.33 ± 0.18 10 
Sc Cdc12-conGFP4 hg 92° 0.02 0.03 1.97 ± 0.51 13 
Sc Cdc12-conGFP4 split 3° 0.01 0.15 1.74 ± 0.23 10 
Sc Cdc12-conGFP0 hg 96° 0.07 0.1 1.39 ± 0.16 11 
Sc Cdc12-conGFP0 split 1° 0.02 0.17 1.49 ± 0.29 10 
Sc Cdc12-conGFP3 hg 175° 0.06 0.07 1.53 ± 0.18 10 
Sc Cdc12-conGFP3 split 96° 0.05 0.22 1.32 ± 0.10 10 
Sc Cdc3-conGFP hg 93° 0.03 0.06 1.51 ± 0.10 10 
Sc Cdc3-conGFP split 2° 0.02 0.11 1.82 ± 0.35 10 
MDCK SEPT2 1-344 fibers 89° 0.05 0.12 1.21 ± 0.08 10 
MDCK SEPT2 1-340 fibers 90° 0.02 0.06 1.27 ± 0.14 10 
MDCK SEPT2 fibers 92° 0.04 0.18 1.17 ± 0.06 10 
Ag Cdc12-conGFP4 FCF 91° 0.01 0.01 3.91 ± 1.39 10 
Ag Cdc3-conGFP fibers 60° 0.01 0.04 1.44 ± 0.32 11 
Ag Cdc3-conGFP fibers 122° 0.03 0.04 1.69 ± 0.33 14 
Strain Type Offset Pop var Ring var polRatio n 
Ag Cdc12-conGFP4 ir 87° 0.04 0.14 1.95 ± 0.47 13 
Ag Cdc12-conGFP4 split 180° 0.17 0.34 1.43 ± 0.18 10 
Ag Cdc12-conGFP0 ir 86° 0.07 0.3 1.52 ± 0.18 11 
Ag Cdc12-conGFP0 split 4° 0.07 0.41 1.27 ± 0.14 10 
Ag Cdc12-conGFP3 ir 0° 0.15 0.27 1.41 ± 0.16 12 
Ag Cdc12-conGFP3 split 88° 0.18 0.48 1.24 ± 0.10 10 
Ag Cdc3-conGFP ir 86° 0.06 0.1 1.90 ± 0.71 11 
Ag Cdc3-conGFP split 180° 0.11 0.24 1.33 ± 0.18 10 
Sc Cdc12-conGFP4 hg 92° 0.02 0.03 1.97 ± 0.51 13 
Sc Cdc12-conGFP4 split 3° 0.01 0.15 1.74 ± 0.23 10 
Sc Cdc12-conGFP0 hg 96° 0.07 0.1 1.39 ± 0.16 11 
Sc Cdc12-conGFP0 split 1° 0.02 0.17 1.49 ± 0.29 10 
Sc Cdc12-conGFP3 hg 175° 0.06 0.07 1.53 ± 0.18 10 
Sc Cdc12-conGFP3 split 96° 0.05 0.22 1.32 ± 0.10 10 
Sc Cdc3-conGFP hg 93° 0.03 0.06 1.51 ± 0.10 10 
Sc Cdc3-conGFP split 2° 0.02 0.11 1.82 ± 0.35 10 
MDCK SEPT2 1-344 fibers 89° 0.05 0.12 1.21 ± 0.08 10 
MDCK SEPT2 1-340 fibers 90° 0.02 0.06 1.27 ± 0.14 10 
MDCK SEPT2 fibers 92° 0.04 0.18 1.17 ± 0.06 10 
Ag Cdc12-conGFP4 FCF 91° 0.01 0.01 3.91 ± 1.39 10 
Ag Cdc3-conGFP fibers 60° 0.01 0.04 1.44 ± 0.32 11 
Ag Cdc3-conGFP fibers 122° 0.03 0.04 1.69 ± 0.33 14 

Ir, “inter-region” septin rings formed along the hyphae of A. gossypii, made of discrete bars (Fig. 1); hg, “hourglass” septin rings in S. cerevisiae, appear contiguous by light microscopy; split, hg or ir rings which have undergone septation; FCF/fibers, septins organized into fibrous structures, “FCF” fibers induced by treatment with FCF; offset, angle between polarized fluorescence and ring axis, averaged over the population of rings analyzed; pop var, “population variance” in the average dipole orientation of the population of rings analyzed. For pop var and ring var, scale = 0–1; 0 = no variance; 1 = complete variance/randomness. ring var, “ring variance” denotes the average variability of dipole coherence across a single structure; each individual ring var was found using weighted averaging, “vars” were arithmetically averaged for final “ring var”. For pop var and ring var, scale = 0–1; 0 = no variance; 1 = complete variance/randomness. polRatio, “polarization ratio” denotes the average strength of order for septin structures; 1 = not ordered.

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