HIF-1α inhibition requires microtubule disruption
| Cell line | 1A9 | 1A9/PTX10 | 1A9 | 1A9/2MRC | ||||
| Ctrl | TX | Ctrl | TX | Ctrl | 2ME2 | Ctrl | 2ME2 | |
| HIF-1α | ||||||||
| Untranslated | 41.3 ± 0.6 | 71.0 ± 2.1 | 6.3 ± 1.5 | 4.5 ± 1.6 | 14.4 ± 1.5 | 52.9 ± 4.3 | 16.9 ± 2.2 | 17.5 ± 1.1 |
| Translated | 58.7 ± 1.6 | 29.0 ± 2.1 | 93.7 ± 1.5 | 95.5 ± 1.6 | 85.6 ± 1.5 | 47.1 ± 4.3 | 83.1 ± 2.2 | 82.5 ± 1.1 |
| GAPDH | ||||||||
| Untranslated | 2.5 ± 1.1 | 6.8 ± 2.9 | 18.8 ± 2.5 | 15.5 ± 4.9 | 12.3 ± 1.5 | 12.6 ± 4.1 | 10.3 ± 1.1 | 4.5 ± 1.8 |
| Translated | 97.5 ± 1.1 | 93.2 ± 2.9 | 81.2 ± 2.5 | 84.5 ± 4.9 | 87.7 ± 1.5 | 87.4 ± 4.1 | 89.7 ± 1.1 | 95.5 ± 1.8 |
| Cell line | 1A9 | 1A9/PTX10 | 1A9 | 1A9/2MRC | ||||
| Ctrl | TX | Ctrl | TX | Ctrl | 2ME2 | Ctrl | 2ME2 | |
| HIF-1α | ||||||||
| Untranslated | 41.3 ± 0.6 | 71.0 ± 2.1 | 6.3 ± 1.5 | 4.5 ± 1.6 | 14.4 ± 1.5 | 52.9 ± 4.3 | 16.9 ± 2.2 | 17.5 ± 1.1 |
| Translated | 58.7 ± 1.6 | 29.0 ± 2.1 | 93.7 ± 1.5 | 95.5 ± 1.6 | 85.6 ± 1.5 | 47.1 ± 4.3 | 83.1 ± 2.2 | 82.5 ± 1.1 |
| GAPDH | ||||||||
| Untranslated | 2.5 ± 1.1 | 6.8 ± 2.9 | 18.8 ± 2.5 | 15.5 ± 4.9 | 12.3 ± 1.5 | 12.6 ± 4.1 | 10.3 ± 1.1 | 4.5 ± 1.8 |
| Translated | 97.5 ± 1.1 | 93.2 ± 2.9 | 81.2 ± 2.5 | 84.5 ± 4.9 | 87.7 ± 1.5 | 87.4 ± 4.1 | 89.7 ± 1.1 | 95.5 ± 1.8 |
Ctrl, control. Polysome association of HIF-1α and GAPDH mRNA in 1A9, 1A9/PTX10, and 1A9/2MRC cells. The percentage of translated versus untranslated mRNA is displayed as mean ± SEM (n = 2).