Table II.

Plasmids used

Name Backbone Content Source or reference 
pLD1 pMHTGal Sic1 Δaa2-50; under GAL1-10 promoter control Noton and Diffley, 2000  
pGal-CLB2[ΔDB] YIplac211 Clb2-Δcdb; under GAL1-10 promoter control A. Amon 
pGal-LTE1-GFP pRS406 LTE1-GFP; under GAL1-10 promoter control Jensen et al., 2002  
pcla4-75-degron  cla4-75-HA degron allele T. Yamamoto 
pHP659 pRS306 BUD1-HA H.O. Park 
pMG-Lte1 pMG1 GST-LTE1; under GAL1-10 promoter control This study; Geymonat et al., 2007  
pMH919-GST pMH919 GST; under GAL1-10 promoter control This study; Geymonat et al., 2007  
pMH14 pRS423 bud1G12V-HA; under GAL1-10 promoter control This study; Geymonat et al., 2007  
pKGFP-Lte1 pKGFP Last 600 bp of LTE1 in frame with GFP Jensen et al., 2002  
pKGFP-Kel1 pKGFP Last 674 bp of KEL1 in frame with GFP This study 
pMG97 pRS304 Last 674 bp of KEL1 in frame with CFP This study 
pMG52 YIplac128 LTE1-HA3 Geymonat et al., 2009  
pMG58 YIplac128 LTE1-GFP Geymonat et al., 2009  
pMG69 pRS406 cdc42G12V; under GAL1-10 promoter control This study 
pMG108 YIplac128 lte1-cdk-HA3 Jensen et al., 2002  
pMG110 pRS306 Last 947 bp of SPA2 in frame with GFP Geymonat et al., 2009  
pMG119 YIplac128 LTE1-ProA This study 
pMG120 YIplac128 lte1-cdk-ProA This study 
pMG180 YIplac128 lte1-8N-GFP Geymonat et al., 2009  
pMG183 YIplac128 lte1-8N-HA3 Geymonat et al., 2009  
pMG184 YIplac128 lte1-8N-ProA This study 
pMG191 YIplac128 lte1-8N-GFP; under GAL1-10 promoter control This study 
pMG213 pRS304 RAS2; under GAL1-10 promoter control This study 
pMG220 YIplac128 lte1-8N-R1343E-GFP; under GAL1-10 promoter control This study 
pMG221 YIplac128 LTE1-GFP; under MET3 promoter This study 
pMG237 pRS306 bud1G12V-HA; under GAL1-10 promoter control This study 
pMG246 pRS306 Last 570 bp of BUD5 in frame with GFP This study 
pMG247 YIplac128 lte1-8N-3HA; under GAL1-10 promoter control This study 
Name Backbone Content Source or reference 
pLD1 pMHTGal Sic1 Δaa2-50; under GAL1-10 promoter control Noton and Diffley, 2000  
pGal-CLB2[ΔDB] YIplac211 Clb2-Δcdb; under GAL1-10 promoter control A. Amon 
pGal-LTE1-GFP pRS406 LTE1-GFP; under GAL1-10 promoter control Jensen et al., 2002  
pcla4-75-degron  cla4-75-HA degron allele T. Yamamoto 
pHP659 pRS306 BUD1-HA H.O. Park 
pMG-Lte1 pMG1 GST-LTE1; under GAL1-10 promoter control This study; Geymonat et al., 2007  
pMH919-GST pMH919 GST; under GAL1-10 promoter control This study; Geymonat et al., 2007  
pMH14 pRS423 bud1G12V-HA; under GAL1-10 promoter control This study; Geymonat et al., 2007  
pKGFP-Lte1 pKGFP Last 600 bp of LTE1 in frame with GFP Jensen et al., 2002  
pKGFP-Kel1 pKGFP Last 674 bp of KEL1 in frame with GFP This study 
pMG97 pRS304 Last 674 bp of KEL1 in frame with CFP This study 
pMG52 YIplac128 LTE1-HA3 Geymonat et al., 2009  
pMG58 YIplac128 LTE1-GFP Geymonat et al., 2009  
pMG69 pRS406 cdc42G12V; under GAL1-10 promoter control This study 
pMG108 YIplac128 lte1-cdk-HA3 Jensen et al., 2002  
pMG110 pRS306 Last 947 bp of SPA2 in frame with GFP Geymonat et al., 2009  
pMG119 YIplac128 LTE1-ProA This study 
pMG120 YIplac128 lte1-cdk-ProA This study 
pMG180 YIplac128 lte1-8N-GFP Geymonat et al., 2009  
pMG183 YIplac128 lte1-8N-HA3 Geymonat et al., 2009  
pMG184 YIplac128 lte1-8N-ProA This study 
pMG191 YIplac128 lte1-8N-GFP; under GAL1-10 promoter control This study 
pMG213 pRS304 RAS2; under GAL1-10 promoter control This study 
pMG220 YIplac128 lte1-8N-R1343E-GFP; under GAL1-10 promoter control This study 
pMG221 YIplac128 LTE1-GFP; under MET3 promoter This study 
pMG237 pRS306 bud1G12V-HA; under GAL1-10 promoter control This study 
pMG246 pRS306 Last 570 bp of BUD5 in frame with GFP This study 
pMG247 YIplac128 lte1-8N-3HA; under GAL1-10 promoter control This study 

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