Table I.

Yeast strains

Strain name Relevant genotype Source or reference 
15D MATa, ura3Δns ade1 his2 leu2-3,112 trp1-1 Richardson et al., 1989  
SY144 MATa lte1::KANR Derived from 15D; Jensen et al., 2002  
SY148 MATa lte1::KANR ura3:: PGAL1-10-LTE1-GFP(URA3) Derived from 15D 
SY157 MATa LTE1::LTE1-3HA cla4::LEU2 Derived from 15D 
SY158 MATa LTE1::LTE1-GFP cla4::LEU2 Derived from 15D 
SY159 MATa KEL1::KEL1-GFP(KANR) Derived from 15D 
SY160 MATa lte1::KANR KEL1::KEL1-GFP(KANR) Derived from 15D 
MGY205 MATa lte1::KANR leu2::LTE1-3HA(LEU2) Derived from 15D 
MGY212 MATa lte1::KANR slk19:: KANR + YCplac33-LTE1 Derived from 15D 
MGY218 MATa lte1::KANR ura3::PGAL1-10-CLB2[Δcdb](URA3) Derived from 15D 
MGY232 MATa ura3::PGAL1-10-CLB2[Δcdb](URA3) Derived from 15D 
MGY239 MATa lte1::KANR leu2::LTE1-3HA(LEU2) ura3::PGAL1-10-CLB2[Δcdb](URA3) Derived from 15D 
MGY240 MATa lte1::KANR leu2::LTE1-Cdk-3HA(LEU2) ura3::PGAL1-10-CLB2[Δcdb](URA3) Derived from 15D 
MGY261 MATa lte1::KANR leu2::LTE1-ProA(LEU2) Derived from 15D 
MGY277 MATa lte1::KANR leu2::LTE1-ProA(LEU2) KEL1::KEL1-3HA(TRP1) + pLD1 Derived from 15D 
MGY281 MATa lte1::KANR leu2::LTE1-Cdk-ProA(LEU2) Derived from 15D 
MGY296 MATa lte1::KANR leu2::GFP-CDC14(LEU2) Derived from 15D 
MGY302 MATa leu2::GFP-CDC14(LEU2) Derived from 15D 
MGY305 MATa LTE1::LTE1-GFP(KANR) KEL1::KEL1-CFP(TRP1) + pLD1 Derived from 15D 
MGY308 MATa KEL1::KEL1-GFP(KANR) SPA2::SPA2-GFP(URA3) Derived from 15D; Geymonat et al., 2009  
MGY309 MATa lte1::KANR KEL1::KEL1-GFP(KANR) SPA2::SPA2-GFP(URA3) Derived from 15D; Geymonat et al., 2009  
MGY313 MATa LTE1::LTE1-3HA(TRP1) cla4::LEU2 ura3::cla4-75dg(URA3) Derived from 15D 
MGY319 MAT lte1::KANR hsl1::LEU2 Derived from 15D 
MGY320 MATa LTE1::LTE1-GFP(KANR) cla4::LEU2 ura3::cla4-75dg(URA3) trp1::PGAL1-10-SIC1[ΔN](TRP1) Derived from 15D 
MGY321 MATa hsl1::LEU2 Derived from 15D 
MGY340 MATa swe1::URA3 KEL1::KEL1-GFP(KANR) Derived from 15D 
MGY341 MATa lte1::KANR swe1::URA3 KEL1::KEL1-GFP(KANR) Derived from 15D 
MGY369 MATa lte1::KANR leu2::LTE1-3HA(LEU2) + pLD1 Derived from 15D 
MGY370 MATa lte1::KANR leu2::LTE1-Cdk-3HA(LEU2)) + pLD1 Derived from 15D 
MGY399 MATa lte1::KANR leu2::LTE1-8N-GFP(LUE2) KEL1::KEL1-CFP(TRP1) + pLD1 Derived from 15D 
MGY423 MATa lte1::KANR cla4::URA3 leu2::LTE1-3HA(LEU2) Derived from 15D 
MGY409 MATa cla4::URA3 leu2::LTE1-8N-GFP(LEU2) Derived from 15D 
MGY415 MATa lte1::KANR leu2::LTE1-8N-PraA(LUE2) Derived from 15D 
MGY443 MATa lte1::KANR cla4::URA3 leu2::LTE1-PraA(LUE2) Derived from 15D 
MGY444 MATa lte1::KANR cla4::URA3 leu2::LTE1-8N-PraA(LUE2) Derived from 15D 
MGY446 MATa lte1::KANR leu2::LTE1-8N-ProA(LEU2) KEL1::KEL1-3HA + pLD1 Derived from 15D 
MGY449 MATa lte1::KANR leu2:: PGAL1-10-LTE1-8N-GFP(LEU2) Derived from 15D 
MGY465 MATa lte1::KANR leu2::LTE1-GFP(LEU2) + pLD1 Derived from 15D 
MGY466 MATa lte1::KANR leu2::LTE1-GFP(LEU2) kel1::TRP1 + pLD1 Derived from 15D 
MGY479 MATa lte1::KANR leu2::LTE1-ProA(LEU2) kel1::TRP1 Derived from 15D 
MGY480 MATa lte1::KANR leu2::LTE1-cdk-ProA(LEU2) kel1::TRP1 Derived from 15D 
MGY485 MATa/α lte1::KANR/lte1::KANR KEL1::KEL1-GFP(KANR)/KEL1 SPA2::SPA2-GFP(URA3)/SPA2 Derived from 15D 
MGY498 MATa lte1::KANR bud1::LEU2 KEL1::KEL1-GFP(KANR) SPA2::SPA2-GFP(URA3) Derived from 15D 
MGY503 MATa lte1::KANR slk19:: KANR kel1::TRP1 + YCplac33-LTE1 Derived from 15D 
MGY516 MATa lte1::KANR bud1::TRP1 Derived from 15D 
MGY517 MATa lte1::KANR leu2:: PGAL1-10-LTE1-8N-GFP(LEU2) trp1:: PGAL1-10-RAS2 (TRP1) Derived from 15D 
MGY518 MATa lte1::KANR ura3:: PGAL1-10-LTE1-GFP(URA3) trp1:: PGAL1-10-RAS2 (TRP1) Derived from 15D 
MGY527 MATa lte1::KANR leu2:: PGAL1-10-LTE1-8N-R1343E-GFP(LEU2) Derived from 15D 
MGY530 MATa lte1::KANR bud1::TRP1 ura3::BUD1-HA(URA3) Derived from 15D 
MGY533 MATa lte1::KANR bud1::TRP1 ura3::BUD1-HA(URA3) leu2::LTE1(LEU2) Derived from 15D 
MGY555 MATa lte1::KANR leu2:: PGAL1-10-LTE1-8N-GFP(LEU2) ura3:: PGAL1-10-BUD1G12V(URA3) Derived from 15D 
MGY562 MATa ura3-1 trp1-289 leu2Δ0 lys2Δ0 his3 mob1::kanMX4 cdc28::LEU2 pep4::LYS2 +pMH919-GST/pMH14-Bud1G12VHA Derived from MGY140; Geymonat et al., 2007  
MGY563 MATa ura3-1 trp1-289 leu2Δ0 lys2Δ0 his3 mob1::kanMX4 cdc28::LEU2 pep4::LYS2 +pMG1-LTE1/pMH14-Bud1G12VHA Derived from MGY140; Geymonat et al., 2007  
MGY565 MATa lte1::KANR leu2::PMET3-LTE1-GFP(LEU2) trp1::PGAL1-10-CLB2[Δcdb](TRP1) Derived from 15D 
MGY567 MATa lte1::KANR cla4::URA3 leu2::LTE1-ProA(LEU2) KEL1::KEL1-3HA(TRP1) Derived from 15D 
MGY574 MATa lte1::KANR leu2::PMET3-LTE1-GFP(LEU2) KEL1::KEL1-CFP(TRP1) ura3:: PGAL1-10-CDC42G12V(URA3) Derived from 15D 
MGY583 MATα dbf2-2 leu2::LTE1-3HA(LEU2) Derived from CG378 
MGY584 MATα dbf2-2 leu2::LTE1-8N-3HA(LEU2) Derived from CG378 
MGY589 MATa lte1::KANR leu2:: PGAL1-10-LTE1-8N-3HA BUD5::BUD5-GFP(URA3) trp1:: PGAL1-10-RAS2 (TRP1) Derived from 15D 
MGY592 MATa LTE1::LTE1-3HA(TRP1) cla4::LEU2 ura3::PGAL1-10-CLB2[Δcdb](URA3) Derived from 15D 
MGY593 MATa lte1:: KANR leu2::PMET3-LTE1-GFP(LEU2) + pLD1 Derived from 15D 
MGY594 MATa lte1:: KANR kel1::TRP1 leu2::PMET3-LTE1-GFP(LEU2) + pLD1 Derived from 15D 
MGY603 MATa lte1::KANR bud3::KANR KEL1::KEL1-CFP(TRP1) ura3::GFP-Tub1(URA3) Derived from 15D 
Strain name Relevant genotype Source or reference 
15D MATa, ura3Δns ade1 his2 leu2-3,112 trp1-1 Richardson et al., 1989  
SY144 MATa lte1::KANR Derived from 15D; Jensen et al., 2002  
SY148 MATa lte1::KANR ura3:: PGAL1-10-LTE1-GFP(URA3) Derived from 15D 
SY157 MATa LTE1::LTE1-3HA cla4::LEU2 Derived from 15D 
SY158 MATa LTE1::LTE1-GFP cla4::LEU2 Derived from 15D 
SY159 MATa KEL1::KEL1-GFP(KANR) Derived from 15D 
SY160 MATa lte1::KANR KEL1::KEL1-GFP(KANR) Derived from 15D 
MGY205 MATa lte1::KANR leu2::LTE1-3HA(LEU2) Derived from 15D 
MGY212 MATa lte1::KANR slk19:: KANR + YCplac33-LTE1 Derived from 15D 
MGY218 MATa lte1::KANR ura3::PGAL1-10-CLB2[Δcdb](URA3) Derived from 15D 
MGY232 MATa ura3::PGAL1-10-CLB2[Δcdb](URA3) Derived from 15D 
MGY239 MATa lte1::KANR leu2::LTE1-3HA(LEU2) ura3::PGAL1-10-CLB2[Δcdb](URA3) Derived from 15D 
MGY240 MATa lte1::KANR leu2::LTE1-Cdk-3HA(LEU2) ura3::PGAL1-10-CLB2[Δcdb](URA3) Derived from 15D 
MGY261 MATa lte1::KANR leu2::LTE1-ProA(LEU2) Derived from 15D 
MGY277 MATa lte1::KANR leu2::LTE1-ProA(LEU2) KEL1::KEL1-3HA(TRP1) + pLD1 Derived from 15D 
MGY281 MATa lte1::KANR leu2::LTE1-Cdk-ProA(LEU2) Derived from 15D 
MGY296 MATa lte1::KANR leu2::GFP-CDC14(LEU2) Derived from 15D 
MGY302 MATa leu2::GFP-CDC14(LEU2) Derived from 15D 
MGY305 MATa LTE1::LTE1-GFP(KANR) KEL1::KEL1-CFP(TRP1) + pLD1 Derived from 15D 
MGY308 MATa KEL1::KEL1-GFP(KANR) SPA2::SPA2-GFP(URA3) Derived from 15D; Geymonat et al., 2009  
MGY309 MATa lte1::KANR KEL1::KEL1-GFP(KANR) SPA2::SPA2-GFP(URA3) Derived from 15D; Geymonat et al., 2009  
MGY313 MATa LTE1::LTE1-3HA(TRP1) cla4::LEU2 ura3::cla4-75dg(URA3) Derived from 15D 
MGY319 MAT lte1::KANR hsl1::LEU2 Derived from 15D 
MGY320 MATa LTE1::LTE1-GFP(KANR) cla4::LEU2 ura3::cla4-75dg(URA3) trp1::PGAL1-10-SIC1[ΔN](TRP1) Derived from 15D 
MGY321 MATa hsl1::LEU2 Derived from 15D 
MGY340 MATa swe1::URA3 KEL1::KEL1-GFP(KANR) Derived from 15D 
MGY341 MATa lte1::KANR swe1::URA3 KEL1::KEL1-GFP(KANR) Derived from 15D 
MGY369 MATa lte1::KANR leu2::LTE1-3HA(LEU2) + pLD1 Derived from 15D 
MGY370 MATa lte1::KANR leu2::LTE1-Cdk-3HA(LEU2)) + pLD1 Derived from 15D 
MGY399 MATa lte1::KANR leu2::LTE1-8N-GFP(LUE2) KEL1::KEL1-CFP(TRP1) + pLD1 Derived from 15D 
MGY423 MATa lte1::KANR cla4::URA3 leu2::LTE1-3HA(LEU2) Derived from 15D 
MGY409 MATa cla4::URA3 leu2::LTE1-8N-GFP(LEU2) Derived from 15D 
MGY415 MATa lte1::KANR leu2::LTE1-8N-PraA(LUE2) Derived from 15D 
MGY443 MATa lte1::KANR cla4::URA3 leu2::LTE1-PraA(LUE2) Derived from 15D 
MGY444 MATa lte1::KANR cla4::URA3 leu2::LTE1-8N-PraA(LUE2) Derived from 15D 
MGY446 MATa lte1::KANR leu2::LTE1-8N-ProA(LEU2) KEL1::KEL1-3HA + pLD1 Derived from 15D 
MGY449 MATa lte1::KANR leu2:: PGAL1-10-LTE1-8N-GFP(LEU2) Derived from 15D 
MGY465 MATa lte1::KANR leu2::LTE1-GFP(LEU2) + pLD1 Derived from 15D 
MGY466 MATa lte1::KANR leu2::LTE1-GFP(LEU2) kel1::TRP1 + pLD1 Derived from 15D 
MGY479 MATa lte1::KANR leu2::LTE1-ProA(LEU2) kel1::TRP1 Derived from 15D 
MGY480 MATa lte1::KANR leu2::LTE1-cdk-ProA(LEU2) kel1::TRP1 Derived from 15D 
MGY485 MATa/α lte1::KANR/lte1::KANR KEL1::KEL1-GFP(KANR)/KEL1 SPA2::SPA2-GFP(URA3)/SPA2 Derived from 15D 
MGY498 MATa lte1::KANR bud1::LEU2 KEL1::KEL1-GFP(KANR) SPA2::SPA2-GFP(URA3) Derived from 15D 
MGY503 MATa lte1::KANR slk19:: KANR kel1::TRP1 + YCplac33-LTE1 Derived from 15D 
MGY516 MATa lte1::KANR bud1::TRP1 Derived from 15D 
MGY517 MATa lte1::KANR leu2:: PGAL1-10-LTE1-8N-GFP(LEU2) trp1:: PGAL1-10-RAS2 (TRP1) Derived from 15D 
MGY518 MATa lte1::KANR ura3:: PGAL1-10-LTE1-GFP(URA3) trp1:: PGAL1-10-RAS2 (TRP1) Derived from 15D 
MGY527 MATa lte1::KANR leu2:: PGAL1-10-LTE1-8N-R1343E-GFP(LEU2) Derived from 15D 
MGY530 MATa lte1::KANR bud1::TRP1 ura3::BUD1-HA(URA3) Derived from 15D 
MGY533 MATa lte1::KANR bud1::TRP1 ura3::BUD1-HA(URA3) leu2::LTE1(LEU2) Derived from 15D 
MGY555 MATa lte1::KANR leu2:: PGAL1-10-LTE1-8N-GFP(LEU2) ura3:: PGAL1-10-BUD1G12V(URA3) Derived from 15D 
MGY562 MATa ura3-1 trp1-289 leu2Δ0 lys2Δ0 his3 mob1::kanMX4 cdc28::LEU2 pep4::LYS2 +pMH919-GST/pMH14-Bud1G12VHA Derived from MGY140; Geymonat et al., 2007  
MGY563 MATa ura3-1 trp1-289 leu2Δ0 lys2Δ0 his3 mob1::kanMX4 cdc28::LEU2 pep4::LYS2 +pMG1-LTE1/pMH14-Bud1G12VHA Derived from MGY140; Geymonat et al., 2007  
MGY565 MATa lte1::KANR leu2::PMET3-LTE1-GFP(LEU2) trp1::PGAL1-10-CLB2[Δcdb](TRP1) Derived from 15D 
MGY567 MATa lte1::KANR cla4::URA3 leu2::LTE1-ProA(LEU2) KEL1::KEL1-3HA(TRP1) Derived from 15D 
MGY574 MATa lte1::KANR leu2::PMET3-LTE1-GFP(LEU2) KEL1::KEL1-CFP(TRP1) ura3:: PGAL1-10-CDC42G12V(URA3) Derived from 15D 
MGY583 MATα dbf2-2 leu2::LTE1-3HA(LEU2) Derived from CG378 
MGY584 MATα dbf2-2 leu2::LTE1-8N-3HA(LEU2) Derived from CG378 
MGY589 MATa lte1::KANR leu2:: PGAL1-10-LTE1-8N-3HA BUD5::BUD5-GFP(URA3) trp1:: PGAL1-10-RAS2 (TRP1) Derived from 15D 
MGY592 MATa LTE1::LTE1-3HA(TRP1) cla4::LEU2 ura3::PGAL1-10-CLB2[Δcdb](URA3) Derived from 15D 
MGY593 MATa lte1:: KANR leu2::PMET3-LTE1-GFP(LEU2) + pLD1 Derived from 15D 
MGY594 MATa lte1:: KANR kel1::TRP1 leu2::PMET3-LTE1-GFP(LEU2) + pLD1 Derived from 15D 
MGY603 MATa lte1::KANR bud3::KANR KEL1::KEL1-CFP(TRP1) ura3::GFP-Tub1(URA3) Derived from 15D 
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