CG, FlyBase gene ID. Analyzed by corner detection and FFT analysis (Fig. 2) and by decision tree machine learning. The decision tree was trained using features from a set of Scar and Arp2/3-RNAi cells. Ranking reflects the position of the phenotype out of the entire list of ∼14,000 genes. Cell number indicates the normalized number of cells present after RNAi treatment, where 1.00 is the plate mean. Depletions resulting in a normalized cell number <0.25 were excluded. Data are from the initial screen and do not include rescreen data. CK1α scored positive in rescreening analysis by both visual and computational analysis. See Materials and methods for a description of analysis and rescreening methods.