Key targets identified from isolation of CLIC-positive fraction
| Target | Location | Link to endocytosis |
| 4F2 cell surface antigen heavy chain/CD98 | PM | Receptor for Galectin3 and Integrin β-1 endocytosis |
| 78-kD glucose-regulated protein | PM/ER | Associates with lipid rafts and receptor for MHCI |
| Actin, cytoplasmic 1 | Cytosol | Drives endocytic carrier formation |
| Alpha-2-HS-glycoprotein/Fetuin | PM/E | Endocytosis mediated by AnnexinA2 |
| Annexin A2 | E | Binds glycoproteins during endocytosis |
| CD109 antigen | PM | Cell surface GPI-AP |
| CD44 antigen | PM | Hyaluronan receptor |
| Collagen | ECM | ECM constituent |
| Fructose-bisphosphate aldolase A | Cytosol | Mediates Snx9-dynamin interaction |
| Galectin-3 | PM | Mediates Integrin β-1 endocytosis |
| Guanine nucleotide-binding protein Gα(i,o) | PM | Involved in STAT3 signaling |
| Integrin β-1 | PM | Receptor for ECM |
| Lysosome-associated membrane glycoprotein 2 | E | Lysosomal membrane regulator |
| Membrane-associated progesterone receptor component 1 | PM/ER | Involved in cholesterol homeostasis, rapidly cleared from the PM by endocytosis |
| Moesin | PM | Links CD44 to cortical actin to drive features in the PM |
| Myoferlin (Fer-1–like protein 3) | PM | Involved in Ca2+-promoted membrane fusion; binds to AHNAK |
| Neuroblast differentiation-associated protein AHNAK | PM | Binds Myoferlin and Dysferlin and with Annexin2 mediates membrane fusion |
| Prolow-density lipoprotein receptor-related protein 1 (LRP1) | PM | Contains both tyrosine and di-leucine sorting motifs; binds saposin on cell surface |
| Prosaposin | PM/E | Co-receptor with LRP1; binds GM1 |
| Rac1 | PM/E | Induces membrane ruffling |
| Rab-11A | E | Endosome recycling |
| Rab-14 | E | Endosome recycling |
| Rab-7a | E | Late endosome fusion and motility |
| Rab-5A | E | Early endosome fusion and motility |
| Rap-1b | PM | Integrin β-1 endocytosis and focal adhesion turnover; mediates STAT3 |
| Receptor expression-enhancing protein 5 | PM | Involved in Rab-mediated membrane trafficking |
| Reticulon-4 | PM/ER | Tubulates membranes; binds NogoR, a GPI-AP at the PM |
| Thy-1 membrane glycoprotein | PM | GPI-AP; regulates FAK signaling |
| Tmp21 | PM | Contains KDEL sequence, traffics to the PM independently from p24 |
| Vimentin | Cytosol | With Annexin A2 regulates FAK signaling |
| Target | Location | Link to endocytosis |
| 4F2 cell surface antigen heavy chain/CD98 | PM | Receptor for Galectin3 and Integrin β-1 endocytosis |
| 78-kD glucose-regulated protein | PM/ER | Associates with lipid rafts and receptor for MHCI |
| Actin, cytoplasmic 1 | Cytosol | Drives endocytic carrier formation |
| Alpha-2-HS-glycoprotein/Fetuin | PM/E | Endocytosis mediated by AnnexinA2 |
| Annexin A2 | E | Binds glycoproteins during endocytosis |
| CD109 antigen | PM | Cell surface GPI-AP |
| CD44 antigen | PM | Hyaluronan receptor |
| Collagen | ECM | ECM constituent |
| Fructose-bisphosphate aldolase A | Cytosol | Mediates Snx9-dynamin interaction |
| Galectin-3 | PM | Mediates Integrin β-1 endocytosis |
| Guanine nucleotide-binding protein Gα(i,o) | PM | Involved in STAT3 signaling |
| Integrin β-1 | PM | Receptor for ECM |
| Lysosome-associated membrane glycoprotein 2 | E | Lysosomal membrane regulator |
| Membrane-associated progesterone receptor component 1 | PM/ER | Involved in cholesterol homeostasis, rapidly cleared from the PM by endocytosis |
| Moesin | PM | Links CD44 to cortical actin to drive features in the PM |
| Myoferlin (Fer-1–like protein 3) | PM | Involved in Ca2+-promoted membrane fusion; binds to AHNAK |
| Neuroblast differentiation-associated protein AHNAK | PM | Binds Myoferlin and Dysferlin and with Annexin2 mediates membrane fusion |
| Prolow-density lipoprotein receptor-related protein 1 (LRP1) | PM | Contains both tyrosine and di-leucine sorting motifs; binds saposin on cell surface |
| Prosaposin | PM/E | Co-receptor with LRP1; binds GM1 |
| Rac1 | PM/E | Induces membrane ruffling |
| Rab-11A | E | Endosome recycling |
| Rab-14 | E | Endosome recycling |
| Rab-7a | E | Late endosome fusion and motility |
| Rab-5A | E | Early endosome fusion and motility |
| Rap-1b | PM | Integrin β-1 endocytosis and focal adhesion turnover; mediates STAT3 |
| Receptor expression-enhancing protein 5 | PM | Involved in Rab-mediated membrane trafficking |
| Reticulon-4 | PM/ER | Tubulates membranes; binds NogoR, a GPI-AP at the PM |
| Thy-1 membrane glycoprotein | PM | GPI-AP; regulates FAK signaling |
| Tmp21 | PM | Contains KDEL sequence, traffics to the PM independently from p24 |
| Vimentin | Cytosol | With Annexin A2 regulates FAK signaling |
E, Endosomes; ECM, extracellular matrix; ER, endoplasmic reticulum; PM, plasma membrane.
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