Mass spectrometry analysis of ECM proteins deposited by MCF-10A cells
| Gene symbol | Protein name | Amino acid residues | Mascot scorea | Number of total peptides detected |
| LAMA3 | Laminin subunit α3 chain (laminin-5 α3 subunit) | 1713 | 715 | 113 |
| LAMB3 | Laminin subunit β3 chain (laminin-5 β3 subunit) | 1170 | 441 | 69 |
| LAMC2 | Laminin subunit γ2 chain (laminin-5 γ2 subunit) | 1193 | 388 | 106 |
| LAMB1 | Laminin subunit β1 chain | 1786 | 79 | 6 |
| LAMC1 | Laminin subunit γ1 chain | 1609 | 96 | 3 |
| AGRN | Agrin | 2026 | 67 | 3 |
| CYR61 | CYR61 (cysteine-rich angiogenic inducer 61) | 381 | 64 | 4 |
| THBS1 | Thrombospondin 1 (THBS1) | 1170 | 135 | 6 |
| VCAN | Versican, proteoglycan PG-M (V3) | 655 | 47 | 2 |
| FGB | Fibrinogen, β chain | 483 | 49 | 1 |
| Gene symbol | Protein name | Amino acid residues | Mascot scorea | Number of total peptides detected |
| LAMA3 | Laminin subunit α3 chain (laminin-5 α3 subunit) | 1713 | 715 | 113 |
| LAMB3 | Laminin subunit β3 chain (laminin-5 β3 subunit) | 1170 | 441 | 69 |
| LAMC2 | Laminin subunit γ2 chain (laminin-5 γ2 subunit) | 1193 | 388 | 106 |
| LAMB1 | Laminin subunit β1 chain | 1786 | 79 | 6 |
| LAMC1 | Laminin subunit γ1 chain | 1609 | 96 | 3 |
| AGRN | Agrin | 2026 | 67 | 3 |
| CYR61 | CYR61 (cysteine-rich angiogenic inducer 61) | 381 | 64 | 4 |
| THBS1 | Thrombospondin 1 (THBS1) | 1170 | 135 | 6 |
| VCAN | Versican, proteoglycan PG-M (V3) | 655 | 47 | 2 |
| FGB | Fibrinogen, β chain | 483 | 49 | 1 |
Factors indicated by boldface are laminin-5 components. MCF-10Aeco cells grown in serum-free MEGM were detached from culture plates with PBS containing 10 mM EDTA. The proteins remaining on the plates were lysed in SDS sample buffer, separated by SDS-PAGE, and analyzed by mass spectrometry. Only ECM proteins with clear mass spectra with scores of P < 0.05 are listed. Laminin-5 subunits are detected as the major ECM components of MCF-10Aeco cells.
Significance threshold of proteins was set at P < 0.05.