Parameters of zyg-1 centrosome movements in various backgrounds
| Parameter measured | zyg-1 (b1) (n = 12) | zyg-1 (oj7) (n = 7) | zyg-1 (oj7); gpr-1/2(RNAi) (n = 4) | zyg-1 (b1) spherical (n = 5) | zyg-1 (b1); par-3 (n = 7) | zyg-1 (oj7); let-99 (n = 11) | zyg-1 (oj7); par-1 (n = 8) |
| Initial centrosome position at NEB (% embryo length) | 52.1 (2.3) | 52.7 (1.9) | 57.9 (2.8) | 52.3 (2.8) | 50.1 (2.8) | 64.5 (1.1) | 55.2 (3.2) |
| Final centrosome position at end of cell cycle (% embryo length) | 63.7 (5.5) | 60.0 (1.3) | 51.3 (1.7) | 61.7 (3.2) | 50.9 (3.4) | 67.4 (1.4) | 42.1 (2.7) |
| Anterior-most centrosome position after NEB (% embryo length) | 50.7 (3.4) | 51.2 (1.9) | 49.8 (1.3) | 49.3 (1.9) | 46.5 (4.2) | 55.0 (0.9) | 38.9 (2.5) |
| Posterior-most centrosome position after NEB (% embryo length) | 70.1 (3.3) | 72.2 (1.0) | 58.0 (2.9) | 67.3 (3.5) | 59.3 (2.5) | 71.8 (0.9) | 61.2 (2.0) |
| Total number of complete AP transits | 5.2 (2.3) | 4.0 (.32) | 0 | 5.0 (1.6) | 3.1 (1.2) | 0 | 0 |
| Parameter measured | |||||||
| Initial centrosome position at NEB (% embryo length) | 52.1 (2.3) | 52.7 (1.9) | 57.9 (2.8) | 52.3 (2.8) | 50.1 (2.8) | 64.5 (1.1) | 55.2 (3.2) |
| Final centrosome position at end of cell cycle (% embryo length) | 63.7 (5.5) | 60.0 (1.3) | 51.3 (1.7) | 61.7 (3.2) | 50.9 (3.4) | 67.4 (1.4) | 42.1 (2.7) |
| Anterior-most centrosome position after NEB (% embryo length) | 50.7 (3.4) | 51.2 (1.9) | 49.8 (1.3) | 49.3 (1.9) | 46.5 (4.2) | 55.0 (0.9) | 38.9 (2.5) |
| Posterior-most centrosome position after NEB (% embryo length) | 70.1 (3.3) | 72.2 (1.0) | 58.0 (2.9) | 67.3 (3.5) | 59.3 (2.5) | 71.8 (0.9) | 61.2 (2.0) |
| Total number of complete AP transits | 5.2 (2.3) | 4.0 (.32) | 0 | 5.0 (1.6) | 3.1 (1.2) | 0 | 0 |
All values are means with SEM given in parentheses.
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