Table I. Centrosome movements in... | Rockefeller University Press

Table I.

Centrosome movements in wild-type, let-99, and par-1

Parameter measured	Control			Laser severing			Genetic severing
	WT (n = 18)	let-99 (n = 17)	par-1 (n = 8)	WT (n = 24)	let-99 (n = 7)	par-1 (n = 8)	spd-1(RNAi) (n = 9)	let-99; spd-1(RNAi) (n = 9)	par-1; spd-1(RNAi) (n = 9)
AP-directed peak velocities
Anterior centrosome (μm/s)	0.29 (0.08)^a^d	0.32 (0.08)^b	0.26 (0.08)^b^d	0.37 (0.18)^a^d	0.50 (.08)^b	0.34 (0.05)^a^d	0.20 (0.05)^a^d	0.25 (0.16)^b	0.20 (0.05)^a^d
Posterior centrosome (μm/s)	0.35 (0.08)^a	0.35 (0.11)^b	0.27 (0.08)^b	0.55 (0.18)^a^c	0.46 (0.11)^b	0.20 (0.08)^a^c	0.30 (0.05)^a^c	0.24 (0.12)^b	0.16 (0.03)^a^c
Overall peak velocities
Anterior velocity (μm/s)	0.58 (0.12)^a	0.53 (0.18)^b	0.83 (0.19)^b	0.56 (0.18)^a^c	0.57 (0.07)^b	0.85 (0.18)^a^c	0.54 (0.11)^a^c	0.40 (0.10)^a	0.89 (0.19)^a^c
Posterior velocity (μm/s)	0.98 (0.18)^a	0.69 (0.31)^b	0.67 (0.19)^b	0.97 (0.30)^a^c	0.78 (0.24)^b	0.65 (0.16)^a^c	0.97 (0.18)^a^c	0.55 (0.21)^a	0.57 (0.24)^a^c
Maximum amplitude of spindle pole rocking
Anterior pole (% embryo height)	14.9 (3.2)^a	12.4 (4.0)^a	20.3 (4.4)^b	13.6 (6.2)^a	25.5 (6.4)^b	24.8 (7.5)^a	18.9 (3.2)^a	12.9 (4.2)^a	20.2 (3.7)^a
Posterior pole (% embryo height)	20.4 (4.9)^a	21.7 (4.8)^a	17.4 (5.1)^b	24.8 (10.2)^a	32.1 (7.1)^b	20.0 (6.1)^a	26.7 (3.1)^a	21.1 (2.6)^a	14.2 (4.7)^a
Spindle measurements
Minimum spindle length (% embryo length)	24.2 (2.6)	24.6 (2.1)	26.5 (2.9)	25.7 (6.6)	26.9 (1.0)	29.5 (3.6)	26.6 (2.3)	25.4 (0.8)	28.3 (2.0)
Maximum spindle length (% embryo length)	50.3 (2.0)	39.2 (1.8)^e	49.0 (4.9)	54.6 (3.1)	49.7 (2.6)^e	55.6 (4.1)	55.1 (1.5)	38.3 (1.1)^e	54.5 (3.4)
Spindle elongation rate (μm/s)	0.06 (0.03)	0.05 (0.03)	0.05 (0.02)	0.49 (0.15)	0.35 (0.13)	0.38 (0.21)	0.12 (0.05)	0.04 (0.02)	0.11 (0.05)

Parameter measured	Control			Laser severing			Genetic severing
	WT (n = 18)	let-99 (n = 17)	par-1 (n = 8)	WT (n = 24)	let-99 (n = 7)	par-1 (n = 8)	spd-1(RNAi) (n = 9)	let-99; spd-1(RNAi) (n = 9)	par-1; spd-1(RNAi) (n = 9)
AP-directed peak velocities
Anterior centrosome (μm/s)	0.29 (0.08)^a^d	0.32 (0.08)^b	0.26 (0.08)^b^d	0.37 (0.18)^a^d	0.50 (.08)^b	0.34 (0.05)^a^d	0.20 (0.05)^a^d	0.25 (0.16)^b	0.20 (0.05)^a^d
Posterior centrosome (μm/s)	0.35 (0.08)^a	0.35 (0.11)^b	0.27 (0.08)^b	0.55 (0.18)^a^c	0.46 (0.11)^b	0.20 (0.08)^a^c	0.30 (0.05)^a^c	0.24 (0.12)^b	0.16 (0.03)^a^c
Overall peak velocities
Anterior velocity (μm/s)	0.58 (0.12)^a	0.53 (0.18)^b	0.83 (0.19)^b	0.56 (0.18)^a^c	0.57 (0.07)^b	0.85 (0.18)^a^c	0.54 (0.11)^a^c	0.40 (0.10)^a	0.89 (0.19)^a^c
Posterior velocity (μm/s)	0.98 (0.18)^a	0.69 (0.31)^b	0.67 (0.19)^b	0.97 (0.30)^a^c	0.78 (0.24)^b	0.65 (0.16)^a^c	0.97 (0.18)^a^c	0.55 (0.21)^a	0.57 (0.24)^a^c
Maximum amplitude of spindle pole rocking
Anterior pole (% embryo height)	14.9 (3.2)^a	12.4 (4.0)^a	20.3 (4.4)^b	13.6 (6.2)^a	25.5 (6.4)^b	24.8 (7.5)^a	18.9 (3.2)^a	12.9 (4.2)^a	20.2 (3.7)^a
Posterior pole (% embryo height)	20.4 (4.9)^a	21.7 (4.8)^a	17.4 (5.1)^b	24.8 (10.2)^a	32.1 (7.1)^b	20.0 (6.1)^a	26.7 (3.1)^a	21.1 (2.6)^a	14.2 (4.7)^a
Spindle measurements
Minimum spindle length (% embryo length)	24.2 (2.6)	24.6 (2.1)	26.5 (2.9)	25.7 (6.6)	26.9 (1.0)	29.5 (3.6)	26.6 (2.3)	25.4 (0.8)	28.3 (2.0)
Maximum spindle length (% embryo length)	50.3 (2.0)	39.2 (1.8)^e	49.0 (4.9)	54.6 (3.1)	49.7 (2.6)^e	55.6 (4.1)	55.1 (1.5)	38.3 (1.1)^e	54.5 (3.4)
Spindle elongation rate (μm/s)	0.06 (0.03)	0.05 (0.03)	0.05 (0.02)	0.49 (0.15)	0.35 (0.13)	0.38 (0.21)	0.12 (0.05)	0.04 (0.02)	0.11 (0.05)

All values are means with SEM given in parentheses.

Statistically significant difference between anterior and posterior spindle poles within a genotype and treatment, P < 0.05 (Student’s t test).

AP values within a genotype and treatment were tested and are not significantly different from each other.

Statistically significant difference between spindle pole velocities in WT and par-1, anterior versus anterior, and posterior versus posterior within a treatment, P < 0.05 (Student’s t test).

The difference between WT and par-1, anterior versus anterior within a treatment, were tested and are not significantly different from each other.

Statistically significant difference between maximum spindle lengths in let-99 embryos, control versus severed, and severed versus spd-1(RNAi), P < 0.05 (Student’s t test).

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