AQUA of IFT81:BBS1 stoichiometry in the flagellar membrane-plus-matrix by MS
| Protein | Peptide | Femtomole synthetic AQUA peptide/femtomole native peptide | ||
| Analysis 1 | Analysis 2 | |||
| IFT81 | AEV#SENETK | 50:16 | 50:14 | |
| IFT81 | LLETL#NEDVK | 50:18 | 50:18 | |
| IFT81 | TSALEGEVSGL#K | 50:22 | 50:22.5 | |
| BBS1 | ADAGLYSL#GK | 50:4 | 50:3 | |
| BBS1 | LYV#EQTQR | 50:3.5 | 50:3 | |
| BBS1 | LQDAGVVL#QTR | 50:2 | 50:2 | |
| Protein | Peptide | Femtomole synthetic AQUA peptide/femtomole native peptide | ||
| Analysis 1 | Analysis 2 | |||
| IFT81 | AEV#SENETK | 50:16 | 50:14 | |
| IFT81 | LLETL#NEDVK | 50:18 | 50:18 | |
| IFT81 | TSALEGEVSGL#K | 50:22 | 50:22.5 | |
| BBS1 | ADAGLYSL#GK | 50:4 | 50:3 | |
| BBS1 | LYV#EQTQR | 50:3.5 | 50:3 | |
| BBS1 | LQDAGVVL#QTR | 50:2 | 50:2 | |
Membrane-plus-matrix was prepared from isolated wild-type flagella and subjected to AQUA analysis. Data represent duplicate MS analyses, each containing 50 fmol of all six of the indicated synthetic AQUA peptides. # follows residues with 13C- or 15N-stable isotope mass tags. The IFT81 mean ratios for analyses 1 and 2 are 50:18.7 and 50:18.17, respectively. The BBS1 mean ratios for analyses 1 and 2 are 50:3 and 50:2.67, respectively. The ratios of IFT81 to BBS1 for analyses 1 and 2 are 6.23:1 and 6.81:1, respectively. The mean (±SD) of the two IFT81:BBS1 ratio values is 6.52:1 (±0.41).