Bioenergetic characterization of wild-type and Δfcj1 cells
| Bioenergetic properties of isolated mitochondria | Wild type | Δfcj1 |
| Uncoupled respiration (nmol oxygen/min/mg of protein) | 248.5 ± 41.27 (n = 3) | 174.6 ± 30.9 (n = 3)a |
| Respiratory control (state III/state IV) | 3.01 ± 0.23 (n = 3) | 2.93 ± 0.09 (n = 3) |
| Membrane potential ΔΨ (ΔF/F) | 0.285 ± 0.019 (n = 3) | 0.221 ± 0.032 (n = 3)b |
| ATPase activity (nmol Pi/min/mg of protein) | ||
| −oligomycin | 1,393 ± 231 (n = 3) | 1,264 ± 121 (n = 3)c |
| +oligomycin | 275 ± 93 (n = 3) | 256 ± 102 (n = 3)d |
| Bioenergetic properties of isolated mitochondria | Wild type | Δfcj1 |
| Uncoupled respiration (nmol oxygen/min/mg of protein) | 248.5 ± 41.27 (n = 3) | 174.6 ± 30.9 (n = 3)a |
| Respiratory control (state III/state IV) | 3.01 ± 0.23 (n = 3) | 2.93 ± 0.09 (n = 3) |
| Membrane potential ΔΨ (ΔF/F) | 0.285 ± 0.019 (n = 3) | 0.221 ± 0.032 (n = 3)b |
| ATPase activity (nmol Pi/min/mg of protein) | ||
| −oligomycin | 1,393 ± 231 (n = 3) | 1,264 ± 121 (n = 3)c |
| +oligomycin | 275 ± 93 (n = 3) | 256 ± 102 (n = 3)d |
F, fluorescence. For measuring bioenergetic properties, mitochondria were isolated from cells grown at 30°C on complete liquid media containing 2% (vol/vol) lactate. Mean values together with standard deviations (±) of n measurements are given.
70% of wild type.
78% of wild type.
91% of wild type.
93% of wild type.