Table III.

Spine head width

Description n (thin spines) Thin spine (mean ± SEM) P-value n (mushroom) Mushroom (mean ± SEM) P-value 
  µm   µm  
GFP control 247 0.51 ± 0.0079  64 0.96 ± 0.024 0.446 
Cdc42 GDP 28 0.59 ± 0.018 <0.001 20 1.01 ± 0.058 0.275a 
Cdc42 GTP 55 0.58 ± 0.014 0.836a 81 1.08 ± 0.032 0.006 
   <0.001    
GFP control 263 0.54 ± 0.0077  81 0.92 ± 0.016  
Inactive Rif 78 0.58 ± 0.013 <0.001 99 1.06 ± 0.028 0.001 
p34 siRNA 22 0.57 ± 0.035 0.261 18 0.93 ± 0.037 0.280 
cof1 siRNA 134 0.56 ± 0.011 0.148 78 0.93 ± 0.018 0.778 
cof1 siRNA + cof1 rescue 263 0.51 ± 0.0072 0.016 48 0.94 ± 0.026 0.897 
   <0.001b   0.813b 
GFP control 197 0.53 ± 0.0091 0.711 66 0.97 ± 0.025 0.182 
Active Rif 102 0.54 ± 0.012  44 1.02 ± 0.035  
GFP control 320 0.52 ± 0.0073  84 0.93 ± 0.017  
mDia2 siRNA 85 0.56 ± 0.012 0.001 58 1.03 ± 0.026 <0.001 
GFP control 225 0.48 ± 0.0087 0.678 37 0.94 ± 0.026 0.698 
Inactive Rif + active mDia2 13 0.50 ± 0.046  16 0.97 ± 0.048  
GFP control 205 0.53 ± 0.0093 0.391 62 0.92 ± 0.021 0.271 
Inactive Rif + WA 22 0.51 ± 0.023  4 0.97 ± 0.039  
W 174 0.58 ± 0.012 0.549 74 1.06 ± 0.025 0.736 
WA 17 0.55 ± 0.040  20 1.07 ± 0.038  
Description n (thin spines) Thin spine (mean ± SEM) P-value n (mushroom) Mushroom (mean ± SEM) P-value 
  µm   µm  
GFP control 247 0.51 ± 0.0079  64 0.96 ± 0.024 0.446 
Cdc42 GDP 28 0.59 ± 0.018 <0.001 20 1.01 ± 0.058 0.275a 
Cdc42 GTP 55 0.58 ± 0.014 0.836a 81 1.08 ± 0.032 0.006 
   <0.001    
GFP control 263 0.54 ± 0.0077  81 0.92 ± 0.016  
Inactive Rif 78 0.58 ± 0.013 <0.001 99 1.06 ± 0.028 0.001 
p34 siRNA 22 0.57 ± 0.035 0.261 18 0.93 ± 0.037 0.280 
cof1 siRNA 134 0.56 ± 0.011 0.148 78 0.93 ± 0.018 0.778 
cof1 siRNA + cof1 rescue 263 0.51 ± 0.0072 0.016 48 0.94 ± 0.026 0.897 
   <0.001b   0.813b 
GFP control 197 0.53 ± 0.0091 0.711 66 0.97 ± 0.025 0.182 
Active Rif 102 0.54 ± 0.012  44 1.02 ± 0.035  
GFP control 320 0.52 ± 0.0073  84 0.93 ± 0.017  
mDia2 siRNA 85 0.56 ± 0.012 0.001 58 1.03 ± 0.026 <0.001 
GFP control 225 0.48 ± 0.0087 0.678 37 0.94 ± 0.026 0.698 
Inactive Rif + active mDia2 13 0.50 ± 0.046  16 0.97 ± 0.048  
GFP control 205 0.53 ± 0.0093 0.391 62 0.92 ± 0.021 0.271 
Inactive Rif + WA 22 0.51 ± 0.023  4 0.97 ± 0.039  
W 174 0.58 ± 0.012 0.549 74 1.06 ± 0.025 0.736 
WA 17 0.55 ± 0.040  20 1.07 ± 0.038  

Statistically significant values (P < 0.050) are shown in bold.

a

P-value for cdc42GDP:cdc42GTP.

b

P-value for cof1 siRNA:cof1 siRNA + cof1 rescue.

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