BAC copy number estimation and average chromatin compaction ratios
| DHFR D10_2 | Hsp70_5 | MT1_1_4 | |
| aCopy number by real-time PCR | 13 (10–16) | 10 (8–14) | 9 (7–11) |
| bCopy number by Fiber-FISH | 11–12 | 9–10 | 16–18 |
| BAC size (kb) | 187.1 | 192.6 | 185.2 |
| Average contour length w/o induction (µm) | 0.65 | 0.52 | 0.69 |
| cCompaction ratio w/o induction | 1125:1 | 1200:1 | 1550:1 |
| Average contour length w/induction (µm) | 1.41 | 1.51 | 0.98 |
| cCompaction ratio w/induction | 520:1 | 410:1 | 1090:1 |
| DHFR D10_2 | Hsp70_5 | MT1_1_4 | |
| aCopy number by real-time PCR | 13 (10–16) | 10 (8–14) | 9 (7–11) |
| bCopy number by Fiber-FISH | 11–12 | 9–10 | 16–18 |
| BAC size (kb) | 187.1 | 192.6 | 185.2 |
| Average contour length w/o induction (µm) | 0.65 | 0.52 | 0.69 |
| cCompaction ratio w/o induction | 1125:1 | 1200:1 | 1550:1 |
| Average contour length w/induction (µm) | 1.41 | 1.51 | 0.98 |
| cCompaction ratio w/induction | 520:1 | 410:1 | 1090:1 |
Mean values obtained from more than three individual experiments. Data ranges are in parentheses. Table S1 lists PCR primers used.
Values obtained by counting the numbers of lac operator FISH signals in at least three nuclei which show the best stretched chromatin fibers.
Compaction ratios were calculated using copy numbers based on the mean values of the Fiber-FISH data.