Parameters of MT shortening in CHO-K1 cells
| At the cell periphery | In internal cytoplasm | |||||||
| Number of observations | aRate of shortening | bRescue frequency | cLength of shortening | Number of observations | aRate of shortening | bRescue frequency | cLength of shortening | |
| μm/min | min−1 | μm | μm/min | min−1 | μm | |||
| Control cells | 200 MTs; 10 cells | 30.0 ± 9.4 | 10.0 ± 1.8 | 2.9 ± 3.4 | N/A | N/A | N/A | N/A |
| EB1/EB3 depleted cells | 118 MTs; 6 cells | 23.4 ± 10.8 | 10.0 ± 1.5 | 2.4 ± 2.5 | 58 MTs; 7 cells | 29.6 ± 11 | 8.1 ± 1.8 | 3.45 ± 2.4 |
| EB1-CΔAc | 102 MTs; 5 cells | 23.7 ± 8.6 | 7.8 ± 2.0 | 2.9 ± 2.3 | 75 MTs; 5 cells | 23.2 ± 8.8 | 6.7 ± 2.0 | 4.3 ± 3.0 |
| At the cell periphery | In internal cytoplasm | |||||||
| Number of observations | aRate of shortening | bRescue frequency | cLength of shortening | Number of observations | aRate of shortening | bRescue frequency | cLength of shortening | |
| μm/min | min−1 | μm | μm/min | min−1 | μm | |||
| Control cells | 200 MTs; 10 cells | 30.0 ± 9.4 | 10.0 ± 1.8 | 2.9 ± 3.4 | N/A | N/A | N/A | N/A |
| EB1/EB3 depleted cells | 118 MTs; 6 cells | 23.4 ± 10.8 | 10.0 ± 1.5 | 2.4 ± 2.5 | 58 MTs; 7 cells | 29.6 ± 11 | 8.1 ± 1.8 | 3.45 ± 2.4 |
| EB1-CΔAc | 102 MTs; 5 cells | 23.7 ± 8.6 | 7.8 ± 2.0 | 2.9 ± 2.3 | 75 MTs; 5 cells | 23.2 ± 8.8 | 6.7 ± 2.0 | 4.3 ± 3.0 |
N/A, not applicable. The analysis was precluded by extremely low catastrophe frequency in internal cell regions.
The shortening rate ± SD was calculated from the histogram of displacements of shortening MT tips between successive frames using subtracted images.
The rescue frequency (the number of transition events over time) was calculated for each cell and was presented as an average ± SD for the cell population.
The length of uninterrupted shortening.