Parameters of MT growth in CHO-K1 cells
| Number of observations | Length of uninterrupted growtha | Growth rateb | Catastrophe frequencyc | |
| μm | μm/min | min−1 | ||
| Expression of shRNA | ||||
| Luciferase; YFP-CLIP-170 | 100 MTs; 7 cells | 15.6 ± 8.8 | 24.3 ± 8.3 | 0.3 ± 0.1 |
| Luciferase; Cy3-tubulin | 37 MTs; 3 cells | 13.4 ± 5.9 | 18.5 ± 5.3 | 0.2 ± 0.1 |
| EB1; YFP-CLIP-170 | 88 MTs; 7 cells | 14.5 ± 6.4 | 21.4 ± 9.4 | 0.3 ± 0.1 |
| EB1/EB3; YFP-CLIP-170 | 101 MTs; 8 cells | 7.3 ± 4.5 | 22.6 ± 8.4 | 3.2 ± 0.9 |
| EB1/EB3; Cy3-tubulin | 56 MTs; 3 cells | 7.4 ± 4.1 | 22.4 ± 5.5 | 3.7 ± 0.7 |
| Rescue experiments in EB1/EB3-depleted cells | ||||
| EB1 rescue; YFP-CLIP-170 | 64 MTs; 7 cells | 15.1 ± 7.0 | 21.1 ± 8.0 | 0.3 ± 0.1 |
| EB1ΔAc rescue; YFP-CLIP-170 | 96 MTs; 10 cells | 16.6 ± 7.3 | 26.9 ± 10.7 | 0.3 ± 0.1 |
| Expression of EB1 mutants | ||||
| EB1-NL (control); YFP-CLIP-170 | 88 MTs; 7 cells | 18.5 ± 7.1 | 22.5 ± 7.1 | 0.2 ± 0.1 |
| EB1-CΔAc; YFP-CLIP-170 | 116 MTs; 9 cells | 4.5 ± 2.9 | 20.3 ± 7.9 | 4.2 ± 1.0 |
| EB1-CΔAc; Cy3-tubulin | 55 MTs; 4 cells | 4.8 ± 3.4 | 18.7 ± 7.8 | 3.5 ± 0.9 |
| Number of observations | Length of uninterrupted growtha | Growth rateb | Catastrophe frequencyc | |
| μm | μm/min | min−1 | ||
| Expression of shRNA | ||||
| Luciferase; YFP-CLIP-170 | 100 MTs; 7 cells | 15.6 ± 8.8 | 24.3 ± 8.3 | 0.3 ± 0.1 |
| Luciferase; Cy3-tubulin | 37 MTs; 3 cells | 13.4 ± 5.9 | 18.5 ± 5.3 | 0.2 ± 0.1 |
| EB1; YFP-CLIP-170 | 88 MTs; 7 cells | 14.5 ± 6.4 | 21.4 ± 9.4 | 0.3 ± 0.1 |
| EB1/EB3; YFP-CLIP-170 | 101 MTs; 8 cells | 7.3 ± 4.5 | 22.6 ± 8.4 | 3.2 ± 0.9 |
| EB1/EB3; Cy3-tubulin | 56 MTs; 3 cells | 7.4 ± 4.1 | 22.4 ± 5.5 | 3.7 ± 0.7 |
| Rescue experiments in EB1/EB3-depleted cells | ||||
| EB1 rescue; YFP-CLIP-170 | 64 MTs; 7 cells | 15.1 ± 7.0 | 21.1 ± 8.0 | 0.3 ± 0.1 |
| EB1ΔAc rescue; YFP-CLIP-170 | 96 MTs; 10 cells | 16.6 ± 7.3 | 26.9 ± 10.7 | 0.3 ± 0.1 |
| Expression of EB1 mutants | ||||
| EB1-NL (control); YFP-CLIP-170 | 88 MTs; 7 cells | 18.5 ± 7.1 | 22.5 ± 7.1 | 0.2 ± 0.1 |
| EB1-CΔAc; YFP-CLIP-170 | 116 MTs; 9 cells | 4.5 ± 2.9 | 20.3 ± 7.9 | 4.2 ± 1.0 |
| EB1-CΔAc; Cy3-tubulin | 55 MTs; 4 cells | 4.8 ± 3.4 | 18.7 ± 7.8 | 3.5 ± 0.9 |
The length of YFP-CLIP-170 or tubulin tracks represents the length of persistent growth.
The growth rate ± SD was calculated from the histogram of displacements of YFP-CLIP-170–positive tips or Cy3-labeled MT ends between successive frames.
The catastrophe frequency (the number of transition events over time) was calculated for each cell and was presented as an average ± SD for the cell population.