Plasmids used in this study
| Construct name . | Tag . | Primer sequence . | Vector . |
|---|---|---|---|
| BD–α-cat 1–906 | BD | Forward, 5′-GGCGAGATCTCCATGACTGCCGTCCACGCAG-3′ | pDEST32 |
| Reverse, 5′-GGCGAGATCTCAGATGCTGTCCATGGCTTT-3′ | |||
| GST–α-cat 1–906 | GST | Forward, 5′-GGCGAGATCTCCATGACTGCCGTCCACGCAG-3′ | pDEST27 |
| Reverse, 5′-GGCGAGATCTCAGATGCTGTCCATGGCTTT-3′ | |||
| BD–α-cat 1–290 | BD | Forward, 5′-GGCGAGATCTCCATGACTGCCGTCCACGCAG-3′ | pDEST32 |
| Reverse, 5′-TCAAATGATTTGTTTATCAAAGTTGTTG-3′ | |||
| GST–α-cat 1–290 | GST | Forward, 5′-GGCGAGATCTCCATGACTGCCGTCCACGCAG-3′ | pDEST27 |
| Reverse, 5′-TCAAATGATTTGTTTATCAAAGTTGTTG-3′ | |||
| BD–α-cat 291–651 | BD | Forward, 5′-ATTATGGACCCCTTGAGCTTC-3′ | pDEST32 |
| Reverse, 5′-TCATCTGACATCAAAGTCTTCAGTC-3′ | |||
| GST–α-cat 291–651 | GST | Forward, 5′-ATTATGGACCCCTTGAGCTTC-3′ | pDEST27 |
| Reverse, 5′-TCATCTGACATCAAAGTCTTCAGTC-3′ | |||
| BD–α-cat 652–906 | BD | Forward, 5′-ACCATGGTCAGAAGCAGGACCAGTGT-3′ | pDEST32 |
| Reverse, 5′-GGCGAGATCTCAGATGCTGTCCATGGCTTT-3′ | |||
| GST–α-cat 652–906 | GST | Forward, 5′-ACCATGGTCAGAAGCAGGACCAGTGT-3′ | pDEST27 |
| Reverse, 5′-GGCGAGATCTCAGATGCTGTCCATGGCTTT-3′ | |||
| BD–α-cat 1–651 | BD | Forward, 5′-GGCGAGATCTCCATGACTGCCGTCCACGCAG-3′ | pDEST32 |
| Reverse, 5′-TCATCTGACATCAAAGTCTTCAGTC-3′ | |||
| GST–α-cat 1–651 | GST | Forward, 5′-GGCGAGATCTCCATGACTGCCGTCCACGCAG-3′ | pDEST27 |
| Reverse, 5′-TCATCTGACATCAAAGTCTTCAGTC-3′ | |||
| BD–α-cat 291–906 | BD | Forward, 5′-ATTATGGACCCCTTGAGCTTC-3′ | pDEST32 |
| Reverse, 5′-GGCGAGATCTCAGATGCTGTCCATGGCTTT-3′ | |||
| GST–α-cat 291–906 | GST | Forward, 5′-ATTATGGACCCCTTGAGCTTC-3′ | pDEST27 |
| Reverse, 5′-GGCGAGATCTCAGATGCTGTCCATGGCTTT-3′ | |||
| AD–β-cat | AD | Forward, 5′-CGAGGATCCGCAATTGCAATGGCTACTCAAGCTGAC-3′ | pDEST22 |
| Reverse, 5′-GAGGATCCCAATTGTTACAGGTCAGTATCAAACC-3′ | |||
| V5–β-cat | V5 | Forward, 5′-CGAGGATCCGCAATTGCAATGGCTACTCAAGCTGAC-3′ | pcDNA3.1/nV5-DEST |
| Reverse, 5′-GAGGATCCCAATTGTTACAGGTCAGTATCAAACC-3′ | |||
| AD-Dyn | AD | Forward, 5′-GCCATGGCGGACCCTAAATA-3′ | pDEST22 |
| Reverse, 5′-TCACTTTCCCAGCCTCTTC-3′ | |||
| V5-Dyn | V5 | Forward, 5′-GCCATGGCGGACCCTAAATA-3′ | pcDNA3.1/nV5-DEST |
| Reverse, 5′-TCACTTTCCCAGCCTCTTC-3′ | |||
| V5-Tbr1 | V5 | Forward, 5′-GCTATGCAGCTGGAGCATTGCCTC-3′ | pcDNA3.1/nV5-DEST |
| Reverse, 5′-GCTGTGCGAGTAGAAGCCATAGTA-3′ | |||
| V5-Arp1 | V5 | Forward, 5′-ATGGAGTCCTACGATGTGATC-3′ | pcDNA3.1/nV5-DEST |
| Reverse, 5′-TTAGAAGGTTTTCCTGTGGATG-3′ | |||
| GST-Dyn | GST | Forward, 5′-GCGGATCCATGGCGGACCCTAAATACG-3′ | pGEX-6P |
| Reverse, 5′-GCGAATTCTCACTTTCCCAGCCTCTTC-3′ | |||
| GST-vinculin | GST | Forward, 5′-GCGATGCCGGTGTTTCACA-3′ | pDEST27 |
| Reverse, 5′-CTACTGGTACCAGGGAGTC-3′ | |||
| HBT–α-cat | HBT | Forward, 5′-TCTAGAATGACTGCCGTCCACGCAG-3′ | PQCXIP-HBT |
| Reverse, 5′-GAATTCTCAGATGCTGTCCATGGCT-3′ | |||
| HBT–α-cat VH1–VH2 | HBT | Forward, 5′-TCTAGAATGACTGCCGTCCACGCAG-3′ | PQCXIP-HBT |
| Reverse, 5′-CGGAATTCTCATCTGACATCAAAGTCTTCAGTC-3′ |
| Construct name . | Tag . | Primer sequence . | Vector . |
|---|---|---|---|
| BD–α-cat 1–906 | BD | Forward, 5′-GGCGAGATCTCCATGACTGCCGTCCACGCAG-3′ | pDEST32 |
| Reverse, 5′-GGCGAGATCTCAGATGCTGTCCATGGCTTT-3′ | |||
| GST–α-cat 1–906 | GST | Forward, 5′-GGCGAGATCTCCATGACTGCCGTCCACGCAG-3′ | pDEST27 |
| Reverse, 5′-GGCGAGATCTCAGATGCTGTCCATGGCTTT-3′ | |||
| BD–α-cat 1–290 | BD | Forward, 5′-GGCGAGATCTCCATGACTGCCGTCCACGCAG-3′ | pDEST32 |
| Reverse, 5′-TCAAATGATTTGTTTATCAAAGTTGTTG-3′ | |||
| GST–α-cat 1–290 | GST | Forward, 5′-GGCGAGATCTCCATGACTGCCGTCCACGCAG-3′ | pDEST27 |
| Reverse, 5′-TCAAATGATTTGTTTATCAAAGTTGTTG-3′ | |||
| BD–α-cat 291–651 | BD | Forward, 5′-ATTATGGACCCCTTGAGCTTC-3′ | pDEST32 |
| Reverse, 5′-TCATCTGACATCAAAGTCTTCAGTC-3′ | |||
| GST–α-cat 291–651 | GST | Forward, 5′-ATTATGGACCCCTTGAGCTTC-3′ | pDEST27 |
| Reverse, 5′-TCATCTGACATCAAAGTCTTCAGTC-3′ | |||
| BD–α-cat 652–906 | BD | Forward, 5′-ACCATGGTCAGAAGCAGGACCAGTGT-3′ | pDEST32 |
| Reverse, 5′-GGCGAGATCTCAGATGCTGTCCATGGCTTT-3′ | |||
| GST–α-cat 652–906 | GST | Forward, 5′-ACCATGGTCAGAAGCAGGACCAGTGT-3′ | pDEST27 |
| Reverse, 5′-GGCGAGATCTCAGATGCTGTCCATGGCTTT-3′ | |||
| BD–α-cat 1–651 | BD | Forward, 5′-GGCGAGATCTCCATGACTGCCGTCCACGCAG-3′ | pDEST32 |
| Reverse, 5′-TCATCTGACATCAAAGTCTTCAGTC-3′ | |||
| GST–α-cat 1–651 | GST | Forward, 5′-GGCGAGATCTCCATGACTGCCGTCCACGCAG-3′ | pDEST27 |
| Reverse, 5′-TCATCTGACATCAAAGTCTTCAGTC-3′ | |||
| BD–α-cat 291–906 | BD | Forward, 5′-ATTATGGACCCCTTGAGCTTC-3′ | pDEST32 |
| Reverse, 5′-GGCGAGATCTCAGATGCTGTCCATGGCTTT-3′ | |||
| GST–α-cat 291–906 | GST | Forward, 5′-ATTATGGACCCCTTGAGCTTC-3′ | pDEST27 |
| Reverse, 5′-GGCGAGATCTCAGATGCTGTCCATGGCTTT-3′ | |||
| AD–β-cat | AD | Forward, 5′-CGAGGATCCGCAATTGCAATGGCTACTCAAGCTGAC-3′ | pDEST22 |
| Reverse, 5′-GAGGATCCCAATTGTTACAGGTCAGTATCAAACC-3′ | |||
| V5–β-cat | V5 | Forward, 5′-CGAGGATCCGCAATTGCAATGGCTACTCAAGCTGAC-3′ | pcDNA3.1/nV5-DEST |
| Reverse, 5′-GAGGATCCCAATTGTTACAGGTCAGTATCAAACC-3′ | |||
| AD-Dyn | AD | Forward, 5′-GCCATGGCGGACCCTAAATA-3′ | pDEST22 |
| Reverse, 5′-TCACTTTCCCAGCCTCTTC-3′ | |||
| V5-Dyn | V5 | Forward, 5′-GCCATGGCGGACCCTAAATA-3′ | pcDNA3.1/nV5-DEST |
| Reverse, 5′-TCACTTTCCCAGCCTCTTC-3′ | |||
| V5-Tbr1 | V5 | Forward, 5′-GCTATGCAGCTGGAGCATTGCCTC-3′ | pcDNA3.1/nV5-DEST |
| Reverse, 5′-GCTGTGCGAGTAGAAGCCATAGTA-3′ | |||
| V5-Arp1 | V5 | Forward, 5′-ATGGAGTCCTACGATGTGATC-3′ | pcDNA3.1/nV5-DEST |
| Reverse, 5′-TTAGAAGGTTTTCCTGTGGATG-3′ | |||
| GST-Dyn | GST | Forward, 5′-GCGGATCCATGGCGGACCCTAAATACG-3′ | pGEX-6P |
| Reverse, 5′-GCGAATTCTCACTTTCCCAGCCTCTTC-3′ | |||
| GST-vinculin | GST | Forward, 5′-GCGATGCCGGTGTTTCACA-3′ | pDEST27 |
| Reverse, 5′-CTACTGGTACCAGGGAGTC-3′ | |||
| HBT–α-cat | HBT | Forward, 5′-TCTAGAATGACTGCCGTCCACGCAG-3′ | PQCXIP-HBT |
| Reverse, 5′-GAATTCTCAGATGCTGTCCATGGCT-3′ | |||
| HBT–α-cat VH1–VH2 | HBT | Forward, 5′-TCTAGAATGACTGCCGTCCACGCAG-3′ | PQCXIP-HBT |
| Reverse, 5′-CGGAATTCTCATCTGACATCAAAGTCTTCAGTC-3′ |
cat, catenin; Dyn, dynamitin.