Suppression of the meiotic arrest of mat-3(or180) by RNAi-mediated depletion of SAC genes
| RNAi . | One cell . | More than one cell . | Ratio of worms laying embryos with more than one cell . |
|---|---|---|---|
| % | |||
| pUC19 | 30 | 0 | 0 |
| mdf-1 | 12 | 18 | 60 |
| spdl-1 | 22 | 8 | 26.7 |
| RNAi . | One cell . | More than one cell . | Ratio of worms laying embryos with more than one cell . |
|---|---|---|---|
| % | |||
| pUC19 | 30 | 0 | 0 |
| mdf-1 | 12 | 18 | 60 |
| spdl-1 | 22 | 8 | 26.7 |
30 L4 mat-3(or180) hermaphrodites fed with HT115 bacteria expressing dsRNA of indicated genes were shifted to 24°C for 24 h, fixed with methanol and acetone, and then stained with 100 ng/ml DAPI. The numbers of worms that lay only embryos in meiotic division (one cell) and those that lay embryos that had entered mitosis (more than one cell) are shown.