Table I. Duration of mitosis in RPE-1... | Rockefeller University Press

Table I.

Duration of mitosis in RPE-1 cells exposed to various drugs that perturb spindle formation (37°C)^{^a}

Drug/concentration	MTs	Duration of mitosis ± SD (N)^{^b}	Approximate hours in mitosis^{^c}	Survival
				%^{^d}
Control	+	18 ± 3 (33)	0.3	100
3.2 μM nocodazole	−	1,140 ± 732 (25)	19	71
250 nM colcemid	−	1,303 ± 362 (24)	22	60
25 nM vinblastine	−	1,784 ± 602 (24)^{^e}	30	26
1 μM vinblastine	−	1,820 ± 530 (37)	30	21
1 μM maytansine	−	1,558 ± 407 (50)	26	50
500 nM taxol	+	744 ± 399 (117)	12	81
5.0 μM S-Trityl-l-cysteine^{^f}	+	1,179 ± 310 (160)	20	84
2.5 μM dimethylenastron^{^g}	+	1,218 ± 424 (241)	20	80
50 nM nocodazole	+	153 ± 58 (200)	3	100
5 nM vinblastine	+	234 ± 140 (180)	4	95
5 nM taxol	+	161 ± 85 (171)	3	99

Drug/concentration	MTs	Duration of mitosis ± SD (N)^b	Approximate hours in mitosis^c	Survival
				%^d
Control	+	18 ± 3 (33)	0.3	100
3.2 μM nocodazole	−	1,140 ± 732 (25)	19	71
250 nM colcemid	−	1,303 ± 362 (24)	22	60
25 nM vinblastine	−	1,784 ± 602 (24)^e	30	26
1 μM vinblastine	−	1,820 ± 530 (37)	30	21
1 μM maytansine	−	1,558 ± 407 (50)	26	50
500 nM taxol	+	744 ± 399 (117)	12	81
5.0 μM S-Trityl-l-cysteine^f	+	1,179 ± 310 (160)	20	84
2.5 μM dimethylenastron^g	+	1,218 ± 424 (241)	20	80
50 nM nocodazole	+	153 ± 58 (200)	3	100
5 nM vinblastine	+	234 ± 140 (180)	4	95
5 nM taxol	+	161 ± 85 (171)	3	99

Minutes from first frame after NEB to first frame including the initial signs of cytokinesis and/or telophase membrane blebbing (in cells that exited mitosis).

N = total numbers of cells (pooled from ≥2 separate long-term recordings obtained on different days).

Mean duration of mitosis in hours.

Percentage of cells that exited mitosis (versus dying in mitosis).

These data are from a single experiment.

An Eg5 inhibitor (and nonnatural derivative of the α–amino acid cysteine).

An Eg5 inhibitor (a new monastrol analogue).

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