Effects of wild-type Plk4 and SBM-Plk4 expression on centriole number
| Day | Cycling cellsa | S phase–arrested cellsb | ||||||||||
| Plk4-EGFP | Plk4-SBM–EGFP | Plk4-EGFP | Plk4-SBM–EGFP | |||||||||
| Total | Mean ± SD | Median | Total | Mean ± SD | Median | Total | Mean ± SD | Median | Total | Mean ± SD | Median | |
| 1 | 100 | 2.9 ± 3.9 | 2.0 | 104 | 3.5 ± 3.4 | 2.0 | 100 | 3.1 ± 3.1 | 2.0 | 100 | 3.9 ± 4.2 | 2.0 |
| 2 | 112 | 2.6 ± 3.0 | 2.0 | 104 | 4.3 ± 4.2c | 3.0 | 101 | 3.5 ± 3.2 | 2.0 | 100 | 4.7 ± 5.3 | 4.0 |
| 3 | 111 | 3.1 ± 2.7 | 2.0 | 103 | 5.3 ± 4.0c | 4.0 | 100 | 3.1 ± 3.7 | 2.0 | 101 | 5.1 ± 4.4c | 4.0 |
| 4 | 113 | 3.0 ± 2.4 | 2.0 | 111 | 5.3 ± 3.4c | 5.0 | 100 | 3.4 ± 3.7 | 2.0 | 100 | 6.1 ± 5.0c | 5.0 |
| 5 | 97 | 3.2 ± 2.8 | 2.0 | 100 | 7.1 ± 5.8c | 6.0 | 100 | 2.8 ± 2.7 | 2.0 | 100 | 6.4 ± 5.2c | 6.0 |
| Day | Cycling cellsa | S phase–arrested cellsb | ||||||||||
| Plk4-EGFP | Plk4-SBM–EGFP | Plk4-EGFP | Plk4-SBM–EGFP | |||||||||
| Total | Mean ± SD | Median | Total | Mean ± SD | Median | Total | Mean ± SD | Median | Total | Mean ± SD | Median | |
| 1 | 100 | 2.9 ± 3.9 | 2.0 | 104 | 3.5 ± 3.4 | 2.0 | 100 | 3.1 ± 3.1 | 2.0 | 100 | 3.9 ± 4.2 | 2.0 |
| 2 | 112 | 2.6 ± 3.0 | 2.0 | 104 | 4.3 ± 4.2c | 3.0 | 101 | 3.5 ± 3.2 | 2.0 | 100 | 4.7 ± 5.3 | 4.0 |
| 3 | 111 | 3.1 ± 2.7 | 2.0 | 103 | 5.3 ± 4.0c | 4.0 | 100 | 3.1 ± 3.7 | 2.0 | 101 | 5.1 ± 4.4c | 4.0 |
| 4 | 113 | 3.0 ± 2.4 | 2.0 | 111 | 5.3 ± 3.4c | 5.0 | 100 | 3.4 ± 3.7 | 2.0 | 100 | 6.1 ± 5.0c | 5.0 |
| 5 | 97 | 3.2 ± 2.8 | 2.0 | 100 | 7.1 ± 5.8c | 6.0 | 100 | 2.8 ± 2.7 | 2.0 | 100 | 6.4 ± 5.2c | 6.0 |
Expression of Plk4-EGFP and Plk4-SBM–EGFP was driven off of the weak SAS-6p. Positive transfected cells were identified by cotransfection with a plasmid expressing the nuclear protein Nlp-EGFP. Total, total cell number; mean and median columns refer to centriole number.
Centrioles were counted beginning 24 h after transfection.
24 h after transfection, cells were treated with aphidicolin/HU to arrest them through the time course of this experiment. Centrioles were quantified beginning 48 h after transfection.
Indicates a significant difference using an unpaired t test (P < 0.001).