Table II.

Comparison of actin-dynamic parameters from control and inhibitor-treated epidermal cells

 Control: Growing cells Nongrowing cells 100 nM LatB 20 or 50 mM BDM 
Elongation rate; µm/s 1.7 ± 0.7 (52)a 1.6 ± 0.8 (57)ND 0.6 ± 0.2 (51)** 1.4 ± 0.5 (34)* 
Severing frequency; breaks/µm/s 0.011 ± 0.009 (50) 0.014 ± 0.011 (47)ND 0.02 ± 0.02 (40)* 0.004 ± 0.005 (30)** 
Max. filament length; µm 14.8 ± 6.4 (78) 10.3 ± 4.4 (61)** 4.4 ± 1.6 (57)** 9.5 ± 5.2 (51)** 
Filament origin; % de novo/end/side 25/42/33 (52) 33/40/26 (57) 29/55/16 (51) 18/53/29 (34) 
Re-growth of severed end; % 4.4 (362 ends) 2.1 (290 ends) 7.7 (154 ends) 2.5 (122 ends) 
Convolutedness 1.6 ± 0.4 (87) 1.6 ± 0.4 (48)ND 1.2 ± 0.2 (54)** 1.5 ± 0.3 (40)ND 
Rate of change of convolutedness; s−1 0.08 ± 0.07 (87) 0.05 ± 0.03 (48)* 0.03 ± 0.01 (54)** 0.02 ± 0.03 (40)** 

ND, Not significantly different from control value by Student's t test; P value >0.05.

*, Significantly different from control value by Student's t test; P value ≤0.02.

**, Significantly different from control value by Student's t test; P value <0.0001.

a

Values given are means ± SD, with (n) = number of filaments in parentheses. See text for details of experimental treatment and measurement.

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