KLP61F turnover during metaphase and anaphase B in spindles of D. melanogaster embryos
| Near pole | Equator | |||||
|---|---|---|---|---|---|---|
| t1/2 in s | % recovery | t1/2 in s | % recovery | |||
| Preanaphase B | 8.8 ± 0.6 (n = 4) | 87 ± 4 (n = 4) | 5.9 ± 0.3 (n = 5) | 108 ± 7 (n = 5) | ||
| Anaphase B | 3.0 ± 0.6 (n = 5) | 51 ± 16a (n = 5) | 5.4 ± 0.2 (n = 4) | 79 ± 10 (n = 4) | ||
| Near pole | Equator | |||||
|---|---|---|---|---|---|---|
| t1/2 in s | % recovery | t1/2 in s | % recovery | |||
| Preanaphase B | 8.8 ± 0.6 ( | 87 ± 4 ( | 5.9 ± 0.3 ( | 108 ± 7 ( | ||
| Anaphase B | 3.0 ± 0.6 ( | 51 ± 16 | 5.4 ± 0.2 ( | 79 ± 10 ( | ||
Note that this apparent decrease in recovery around the poles is due to the net loss of both tubulin and KLP61F in this region rather than being due to a stable population of KLP61F that turns over slowly (see text).