GFP–IC-2C dynein motility in PC12 cell neurites
. | Normal . | siRNA . | NGF . |
|---|---|---|---|
| Number of videos | 79 | 21 | 16 |
| Moving dynein puncta | |||
| Anterograde number (percent of total) | 63 (66%) | 35 (58%) | 14 (48%) |
| Retrograde number (percent of total) | 33 (34%) | 24 (42%) | 15 (52%) |
| Total number | 96 | 60 | 29 |
| Mean anterograde velocity (μm/s) [n] | 0.9 ± 0.02 [849] | 0.7 ± 0.02 [444] | 0.9 ± 0.03 [173] |
| Mean retrograde velocity (μm/s) [n] | 0.9 ± 0.03 [300] | 0.8 ± 0.03 [329] | 1.2 ± 0.05 [140] |
| Observation time (s) | 1,600 | 320 | 420 |
| Frequency (motile puncta per second) | 0.049 | 0.050 | 0.050 |
. | Normal . | siRNA . | NGF . |
|---|---|---|---|
| Number of videos | 79 | 21 | 16 |
| Moving dynein puncta | |||
| Anterograde number (percent of total) | 63 (66%) | 35 (58%) | 14 (48%) |
| Retrograde number (percent of total) | 33 (34%) | 24 (42%) | 15 (52%) |
| Total number | 96 | 60 | 29 |
| Mean anterograde velocity (μm/s) [n] | 0.9 ± 0.02 [849] | 0.7 ± 0.02 [444] | 0.9 ± 0.03 [173] |
| Mean retrograde velocity (μm/s) [n] | 0.9 ± 0.03 [300] | 0.8 ± 0.03 [329] | 1.2 ± 0.05 [140] |
| Observation time (s) | 1,600 | 320 | 420 |
| Frequency (motile puncta per second) | 0.049 | 0.050 | 0.050 |
GFP–IC-2C dynein puncta moving in PC12 cell neurites were classified according to their direction of movement; the number of puncta in each category and their relative percentages are indicated. Interval velocities were calculated for every movement between each pair of video frames and averaged. The mean velocity (μm/s) and number of movements for each category are indicated. Normal, PC12 cells grown under standard conditions; NGF, data collected from PC12 cells within 10 min of addition of NGF to starved cells; siRNA, PC12 cells transfected with the gene 2 siRNA oligonucleotide.