Lateral pseudopod formation by cells crawling in buffer and in a spatial cAMP gradient
| Cell strain | Number of cells | 0–2 lateral pseudopods per 10 min | 3–5 lateral pseudopods per 10 min | >5 lateral pseudopods per 10 min | Mean frequency of lateral pseudopods per cell per 10 min |
|---|---|---|---|---|---|
| % | % | % | |||
| Buffer | |||||
| AX2 | 31 | 9.6 | 45.16 | 45.16 | 5.19 |
| gefQ− | 33 | 0 | 21.21 | 78.78 | 7.03 |
| cAMP gradient | |||||
| AX2 | 35 | 82.85 | 17.14 | 0 | 1.71 |
| gefQ− | 29 | 6.9 | 65.5 | 27.50 | 4.65 |
| Cell strain | Number of cells | 0–2 lateral pseudopods per 10 min | 3–5 lateral pseudopods per 10 min | >5 lateral pseudopods per 10 min | Mean frequency of lateral pseudopods per cell per 10 min |
|---|---|---|---|---|---|
| Buffer | |||||
| AX2 | 31 | 9.6 | 45.16 | 45.16 | 5.19 |
| | 33 | 0 | 21.21 | 78.78 | 7.03 |
| cAMP gradient | |||||
| AX2 | 35 | 82.85 | 17.14 | 0 | 1.71 |
| | 29 | 6.9 | 65.5 | 27.50 | 4.65 |
Images were taken at a magnification of 40× every 6 s. In all cases, cells were analyzed for 10 min. χ2 test, performed between AX2 and gefQ− cells on data of the three categories of lateral pseudopods formed, showed highly significant (P > 0.001) difference between AX2 and gefQ− cells in both buffer and a cAMP gradient.