Summary of in vitro antigen presentation experiments
| Peptide dose . | Ratio KO/WT . | SEM . | n . |
|---|---|---|---|
| μg/ml | |||
| 1 | 0.82 | 0.24 | 5 |
| 0.3 | 0.41 | 0.21 | 3 |
| 0.1 | 0.51 | 0.26 | 3 |
| 0.03 | 0.22 | 0.22 | 2 |
| 0.01 | 0 | 0 | 3 |
| Peptide dose . | Ratio KO/WT . | SEM . | n . |
|---|---|---|---|
| μg/ml | |||
| 1 | 0.82 | 0.24 | 5 |
| 0.3 | 0.41 | 0.21 | 3 |
| 0.1 | 0.51 | 0.26 | 3 |
| 0.03 | 0.22 | 0.22 | 2 |
| 0.01 | 0 | 0 | 3 |
DCs derived from spinophilin WT and KO mice were loaded with peptide antigen (ovalbumin peptide aa 323–339 or I-Eα 52–68) and then cocultured with CD4+ T cells transgenic for the appropriate TCR (OT-II or DO.11 for ovalbumin or 1H3.1 for I-Eα, respectively). After 18 h, supernatants were isolated and assayed for IL-2 by ELISA. Values shown are the ratio of the response from T cells cocultured with KO/WT DCs. One animal in each group was used for each experiment. The number of experiments (n) is indicated.