Chemotaxis of cells lacking PI3K, PLA2, and sGC
| Strain | Chemotaxis | Speed | Persistence | Roundness | ||
|---|---|---|---|---|---|---|
| % | CI | μm/min | ||||
| Wild type | 92 ± 5 | 0.73 ± 0.05 | 9.1 ± 0.7 | 0.90 ± 0.03 | 0.30 ± 0.023 | |
| Gc null | 80 ± 3 | 0.64 ± 0.03 | 7.8 ± 0.4 | 0.76 ± 0.02 | 0.33 ± 0.036 | |
| Gc null + LY + BPB | 11 ± 5 | 0.19 ± 0.04 | 7.9 ± 0.3 | 0.43 ± 0.02 | 0.54 ± 0.030 | |
| sgc/pla2 null | 82 ± 9 | 0.74 ± 0.02 | 7.6 ± 0.5 | 0.85 ± 0.01 | 0.36 ± 0.036 | |
| sgc/pla2 null + LY | 10 ± 4 | 0.22 ± 0.04 | 5.0 ± 0.4 | 0.43 ± 0.03 | 0.46 ± 0.036 | |
| Strain | Chemotaxis | Speed | Persistence | Roundness | ||
|---|---|---|---|---|---|---|
| Wild type | 92 ± 5 | 0.73 ± 0.05 | 9.1 ± 0.7 | 0.90 ± 0.03 | 0.30 ± 0.023 | |
| 80 ± 3 | 0.64 ± 0.03 | 7.8 ± 0.4 | 0.76 ± 0.02 | 0.33 ± 0.036 | ||
| 11 ± 5 | 0.19 ± 0.04 | 7.9 ± 0.3 | 0.43 ± 0.02 | 0.54 ± 0.030 | ||
| 82 ± 9 | 0.74 ± 0.02 | 7.6 ± 0.5 | 0.85 ± 0.01 | 0.36 ± 0.036 | ||
| 10 ± 4 | 0.22 ± 0.04 | 5.0 ± 0.4 | 0.43 ± 0.03 | 0.46 ± 0.036 | ||
The chemotaxis index (CI), speed, persistence (displacement divided by the length of the path), and roundness (short axis divided by the long axis of a cell considered as an ellipse) were determined for the conditions indicated using the modified Zigmond chamber with 1 μM cAMP in the source. LY is the PI3K inhibitor LY294002; BPB is the PLA2 inhibitor p-BPB. The data shown are the means and SEM of 24–33 cells. The first chemotaxis column shows the chemotactic activity measured with the population assay.
Sharing content requires targeting cookies to be enabled. Please update your cookie preferences to use this feature.