Table II. Inactivation of Akt or APC2... | Rockefeller University Press

Table II.

Inactivation of Akt or APC2 leads to defects in centrosome migration

	Wild type	Control injected	akt mutant	α-Akt injected	Apc2^ΔS mutant	sgg¹/FM7;; akt mutant
Mean angle of centrosome separation	170	168	155	157	161	168
SEM centrosome separation	0.79	1.04	1.77	1.63	2.02	0.73
Percentage of nuclei with centrosome angle <150°	2	8	27	30	16	2

	Wild type	Control injected	akt mutant	α-Akt injected	Apc2^ΔS mutant	sgg¹/FM7;; akt mutant
Mean angle of centrosome separation	170	168	155	157	161	168
SEM centrosome separation	0.79	1.04	1.77	1.63	2.02	0.73
Percentage of nuclei with centrosome angle <150°	2	8	27	30	16	2

Cycle 12 embryos expressing α-tubulin–GFP were imaged every 10 s under the confocal microscope. Image series were analyzed using automated tracking software and the angle of centrosome separation 20 s before NEB was calculated. akt mutant, embryos laid by akt⁰⁴²²⁶/akt^1q mothers; α-Akt injected, embryos injected with Alexa 555–labeled anti-Akt antibodies; apc2^Δsmutant, embryos laid by apc2^Δshomozygote mothers; control injected, embryos injected with Alexa 555–labeled BSA; sgg¹/FM7;; akt mutant, embryos laid by akt⁰⁴²²⁶/akt^1q mothers carrying one copy of the sgg mutation; wild type, wild-type embryos.

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