Table 5. Gene and primer sequences used... | Rockefeller University Press

Table 5.

Gene and primer sequences used for the Y1H experiment

Gene	Primers	Annealing temperature
Crq FP-hind3	5′-CCCAAGCTTTGCTTACAGTGCTGTTACAG-3′	61.5°C
Crq RP-kpn1	5′-CGGGGTACCGGTGGATACGTAGGATTCC-3′	61.5°C
E93gibson-For	5′-AGTACCCATACGACGTACCAGATTACGCTCAGATGGCCGATTGTTCATATGT-3′	64°C
E93gibson_Rev	5′-TTCAGTATCTACGATTCATCTGCAGCTCGACTATAACTGATCCGCCTGGT-3′	64°C

Gene	Primers	Annealing temperature
Crq FP-hind3	5′-CCCAAGCTTTGCTTACAGTGCTGTTACAG-3′	61.5°C
Crq RP-kpn1	5′-CGGGGTACCGGTGGATACGTAGGATTCC-3′	61.5°C
E93gibson-For	5′-AGTACCCATACGACGTACCAGATTACGCTCAGATGGCCGATTGTTCATATGT-3′	64°C
E93gibson_Rev	5′-TTCAGTATCTACGATTCATCTGCAGCTCGACTATAACTGATCCGCCTGGT-3′	64°C

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