Methods resources used in this study
| Reagent or resource | Source | Identifier |
|---|---|---|
| Bacterial and virus strains | ||
| E. coli XL10-GOLD KanR ultracompetent cells | Agilent | 200317 |
| E. coli DH10EMBacY competent cells | Geneva Biotech | DH10EMBacY |
| Chemicals, peptides, and recombinant proteins | ||
| Deuterium oxide 99.9% | Sigma-Aldrich | 151882-10X1ML |
| ATP | Sigma-Aldrich | A7699-1g |
| Protease inhibitor cocktail | Sigma-Aldrich | 535140 |
| Polyoxyethylene (10) lauryl ether | Sigma-Aldrich | P9769 |
| Phosphatidylserine (porcine brain) | Avanti | 840032C |
| Phosphatidylethanolamine (egg yolk) | Sigma-Aldrich | P6386 |
| Phosphatidylcholine (egg yolk) | Avanti | 840051C |
| PI3P (synthetic) | Avanti | 850150-500UG |
| Polyethanolamine | Polysciences | 24765 |
| Critical commercial assays | ||
| Transcreener ADP2 FI assay (1,000 Assay, 384 Well), | BellBrook Labs | 3013-1K |
| Recombinant DNA | ||
| PIKfyveCC construct (pLIB) | This paper | MJ290 |
| Software and algorithms | ||
| HDExaminer | Sierra Analytics | http://massspec.com/hdexaminer |
| GraphPad Prism 7 | GraphPad | https://www.graphpad.com |
| Adobe Illustrator | Adobe | https://www.adobe.com/products/illustrator.html |
| PyMOL | Schroedinger | http://pymol.org |
| Other | ||
| Sf9 insect cells for expression | Expression Systems | 94-001S |
| Reagent or resource | Source | Identifier |
|---|---|---|
| Bacterial and virus strains | ||
| Agilent | 200317 | |
| Geneva Biotech | DH10EMBacY | |
| Chemicals, peptides, and recombinant proteins | ||
| Deuterium oxide 99.9% | Sigma-Aldrich | 151882-10X1ML |
| ATP | Sigma-Aldrich | A7699-1g |
| Protease inhibitor cocktail | Sigma-Aldrich | 535140 |
| Polyoxyethylene (10) lauryl ether | Sigma-Aldrich | P9769 |
| Phosphatidylserine (porcine brain) | Avanti | 840032C |
| Phosphatidylethanolamine (egg yolk) | Sigma-Aldrich | P6386 |
| Phosphatidylcholine (egg yolk) | Avanti | 840051C |
| PI3P (synthetic) | Avanti | 850150-500UG |
| Polyethanolamine | Polysciences | 24765 |
| Critical commercial assays | ||
| Transcreener ADP2 FI assay (1,000 Assay, 384 Well), | BellBrook Labs | 3013-1K |
| Recombinant DNA | ||
| PIKfyveCC construct (pLIB) | This paper | MJ290 |
| Software and algorithms | ||
| HDExaminer | Sierra Analytics | |
| GraphPad Prism 7 | GraphPad | |
| Adobe Illustrator | Adobe | |
| PyMOL | Schroedinger | |
| Other | ||
| Sf9 insect cells for expression | Expression Systems | 94-001S |
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