Drug treatment reagents and concentrations
| Drug | Description | Vehicle | Final concentration |
|---|---|---|---|
| Y-27632 (Cat#281642; Santa Cruz) | RoK inhibitor (Myosin) | H2O | 25 and 100 mM |
| CK666 (Cat#3950; TOCRIS) | Arp2/3 inhibitor | DMSO diluted 1:20 in H2O | 250 µm |
| SMIFH2 (Cat#4401; TOCRIS) | Formin inhibitor (Diaphanous) | DMSO diluted 1:50 in H2O | 50 µm |
| Drug | Description | Vehicle | Final concentration |
|---|---|---|---|
| Y-27632 (Cat#281642; Santa Cruz) | RoK inhibitor (Myosin) | H2O | 25 and 100 mM |
| CK666 (Cat#3950; TOCRIS) | Arp2/3 inhibitor | DMSO diluted 1:20 in H2O | 250 µm |
| SMIFH2 (Cat#4401; TOCRIS) | Formin inhibitor (Diaphanous) | DMSO diluted 1:50 in H2O | 50 µm |
Note: For the most severe inhibition we used (Y-27632 100 mM), the ventral furrow fails to form. Otherwise, mesoderm invagination proceeds as normal in all the backgrounds we used.
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