Key resources table
| Reagent or resource . | Source . | Identifier . |
|---|---|---|
| Antibodies | ||
| Anti-α-catenin | Cell Signaling | Cat#3236 |
| Anti-α-catenin (5B11) | Hybridoma | Daugherty et al. (2014) |
| LZTS2 rabbit polyclonal antibody | Proteintech | 15677-1-AP |
| Acetylated-tubulin (6-11B-1) mouse monoclonal | Sigma-Aldrich | T7451 |
| Vinculin (mouse monoclonal) | Sigma-Aldrich | V9131 |
| Phalloidin-far red | Invitrogen | A22287 |
| GAPDH | Santa Cruz | Cat# sc-25778 |
| Anti-GFP | Invitrogen | A11122 |
| Alexa Fluor 568 phalloidin | Invitrogen | Cat#A12380 |
| Alexa Fluor 488 phalloidin | Invitrogen | Cat#A12379 |
| Goat anti-rabbit Alexa Fluor 488 | Invitrogen | Cat#A11008 |
| Goat anti-mouse Alexa Fluor 568 | Invitrogen | Cat#A11004 |
| HRP-conjugated goat anti-mouse | BioRad | Cat#1706516 |
| HRP-conjugated goat anti-rabbit | BioRad | Cat#1706515 |
| Donkey anti-mouse IR680RD or IR800CW | LiCor Biosciences | P/N 925-68072 |
| Donkey anti-rabbit R680RD or IR800CW | LiCor Biosciences | P/N: 926-32213 |
| Chemicals, peptides, and recombinant proteins | ||
| Hoechst 33342 | Thermo Fisher Scientific | Cat#62249 |
| GFP-Trap magnetic beads | ChromoTek | Cat# gtma-20 |
| MISSION pLKO.1 puro shRNA (targets no mammalian genes) | Millipore Sigma | SHC002 |
| LZTS2 shRNA (human/canine CDS-targeting), 5′-CCGGCTCTGGAAAGCTGGAGAAGAACTCGAGTTCTTCTCCAGCTTTCCAGAGTTTTT-3′ | Millipore Sigma | TRCN0000021126 |
| LZTS2 siRNA Silencer select, 5′-AAGCAGUGAUGUUGAGGAUTT-3′ | Thermo Fisher Scientific | S39015 |
| LZTS2 siRNA Silencer select, 5′-CAGGUGAUCCGCUACCAGATT-3′ | Thermo Fisher Scientific | S39013 |
| Silencer select negative control #1 | Thermo Fisher Scientific | 4390843 |
| ProLong gold Antifade mountant | Thermo Fisher Scientific | Cat#P36934 |
| Premium select fetal bovine serum | R&D Systems | Cat#S11550 |
| Experimental models: Organisms/Strains | ||
| Madin-darby canine kidney II cells | Gumbiner Lab (1996) | Heidelberg strain (Kai Simmons Lab) |
| A431D cells | Troyanovsky Lab | E-cad negative variant of A431 epidermoid skin cancer |
| Software and algorithms | ||
| FIJI/ImageJ (version: 2.1.0/1.53c) | Schneider et al. (2012) | https://imagej.nih.gov/ij/ |
| GraphPad Prism | https://www.graphpad.com/scientific-software/prism/ | |
| Other | ||
| Axioplan2 epifluorescence microscope | Zeiss; 20× objective (Air) | AxioCAM HR camera with AxioVision 4.8 software |
| A1R confocal | Nikon Instruments | Laser point Scanning, GaAsP detectors, Plan-Apochromat 60×/1.4 objective |
| AXR confocal | Nikon Instruments | Resonant scanning, GaAsP detectors, Apochromat-TIRF 60×/1.49 objective |
| Ti2 Widefield Eclipse | Nikon Instruments | Living imaging cell division experiments |
| Plasmids | ||
| LV-monomeric EGFP-αE-catenin (WT, missense, M-domain and ABD mutants) | Gottardi Lab | VectorBuilder |
| LV-mEGFP:hCTNNA1_Wild-type | Gottardi Lab | VectorBuilder; Quinn et al. (2024) |
| LV-mEGFP:hCTNNA1_H0-ABD+ | (RAIM669–672>GSGS) | VectorBuilder; Quinn et al. (2024) |
| LV-mEGFP:hCTNNA1_ΔM-domain | Gottardi Lab, Δ273–635 | VectorBuilder; first used here |
| LV-mEGFP:hCTNNA1_ΔM1-domain | Gottardi Lab, Δ271–396 | VectorBuilder; first used here |
| LV-mEGFP:hCTNNA1_ΔM2,3-domain | Gottardi Lab, Δ397–509 | VectorBuilder; first used here |
| LV-mEGFP:hCTNNA1_ΔNii domain | Gottardi Lab | VectorBuilder; first used here |
| LV-mEGFP:hCTNNA1_R551A | Gottardi Lab | VectorBuilder; M2–M3 salt-bridge disrupting mutant |
| LV-mEGFP:hCTNNA1_L436P | Gottardi Lab | VectorBuilder;Ctnna1 eye phenotype via forward genetic screen |
| LV-mEGFP:hCTNNA1_E307K | Gottardi Lab | VectorBuilder;CTNNA1 butterfly eye mutant |
| Reagent or resource . | Source . | Identifier . |
|---|---|---|
| Antibodies | ||
| Anti-α-catenin | Cell Signaling | Cat#3236 |
| Anti-α-catenin (5B11) | Hybridoma | Daugherty et al. (2014) |
| LZTS2 rabbit polyclonal antibody | Proteintech | 15677-1-AP |
| Acetylated-tubulin (6-11B-1) mouse monoclonal | Sigma-Aldrich | T7451 |
| Vinculin (mouse monoclonal) | Sigma-Aldrich | V9131 |
| Phalloidin-far red | Invitrogen | A22287 |
| GAPDH | Santa Cruz | Cat# sc-25778 |
| Anti-GFP | Invitrogen | A11122 |
| Alexa Fluor 568 phalloidin | Invitrogen | Cat#A12380 |
| Alexa Fluor 488 phalloidin | Invitrogen | Cat#A12379 |
| Goat anti-rabbit Alexa Fluor 488 | Invitrogen | Cat#A11008 |
| Goat anti-mouse Alexa Fluor 568 | Invitrogen | Cat#A11004 |
| HRP-conjugated goat anti-mouse | BioRad | Cat#1706516 |
| HRP-conjugated goat anti-rabbit | BioRad | Cat#1706515 |
| Donkey anti-mouse IR680RD or IR800CW | LiCor Biosciences | P/N 925-68072 |
| Donkey anti-rabbit R680RD or IR800CW | LiCor Biosciences | P/N: 926-32213 |
| Chemicals, peptides, and recombinant proteins | ||
| Hoechst 33342 | Thermo Fisher Scientific | Cat#62249 |
| GFP-Trap magnetic beads | ChromoTek | Cat# gtma-20 |
| MISSION pLKO.1 puro shRNA (targets no mammalian genes) | Millipore Sigma | SHC002 |
| LZTS2 shRNA (human/canine CDS-targeting), 5′-CCGGCTCTGGAAAGCTGGAGAAGAACTCGAGTTCTTCTCCAGCTTTCCAGAGTTTTT-3′ | Millipore Sigma | TRCN0000021126 |
| LZTS2 siRNA Silencer select, 5′-AAGCAGUGAUGUUGAGGAUTT-3′ | Thermo Fisher Scientific | S39015 |
| LZTS2 siRNA Silencer select, 5′-CAGGUGAUCCGCUACCAGATT-3′ | Thermo Fisher Scientific | S39013 |
| Silencer select negative control #1 | Thermo Fisher Scientific | 4390843 |
| ProLong gold Antifade mountant | Thermo Fisher Scientific | Cat#P36934 |
| Premium select fetal bovine serum | R&D Systems | Cat#S11550 |
| Experimental models: Organisms/Strains | ||
| Madin-darby canine kidney II cells | Gumbiner Lab (1996) | Heidelberg strain (Kai Simmons Lab) |
| A431D cells | Troyanovsky Lab | E-cad negative variant of A431 epidermoid skin cancer |
| Software and algorithms | ||
| FIJI/ImageJ (version: 2.1.0/1.53c) | Schneider et al. (2012) | https://imagej.nih.gov/ij/ |
| GraphPad Prism | https://www.graphpad.com/scientific-software/prism/ | |
| Other | ||
| Axioplan2 epifluorescence microscope | Zeiss; 20× objective (Air) | AxioCAM HR camera with AxioVision 4.8 software |
| A1R confocal | Nikon Instruments | Laser point Scanning, GaAsP detectors, Plan-Apochromat 60×/1.4 objective |
| AXR confocal | Nikon Instruments | Resonant scanning, GaAsP detectors, Apochromat-TIRF 60×/1.49 objective |
| Ti2 Widefield Eclipse | Nikon Instruments | Living imaging cell division experiments |
| Plasmids | ||
| LV-monomeric EGFP-αE-catenin (WT, missense, M-domain and ABD mutants) | Gottardi Lab | VectorBuilder |
| LV-mEGFP:hCTNNA1_Wild-type | Gottardi Lab | VectorBuilder; Quinn et al. (2024) |
| LV-mEGFP:hCTNNA1_H0-ABD+ | (RAIM669–672>GSGS) | VectorBuilder; Quinn et al. (2024) |
| LV-mEGFP:hCTNNA1_ΔM-domain | Gottardi Lab, Δ273–635 | VectorBuilder; first used here |
| LV-mEGFP:hCTNNA1_ΔM1-domain | Gottardi Lab, Δ271–396 | VectorBuilder; first used here |
| LV-mEGFP:hCTNNA1_ΔM2,3-domain | Gottardi Lab, Δ397–509 | VectorBuilder; first used here |
| LV-mEGFP:hCTNNA1_ΔNii domain | Gottardi Lab | VectorBuilder; first used here |
| LV-mEGFP:hCTNNA1_R551A | Gottardi Lab | VectorBuilder; M2–M3 salt-bridge disrupting mutant |
| LV-mEGFP:hCTNNA1_L436P | Gottardi Lab | VectorBuilder;Ctnna1 eye phenotype via forward genetic screen |
| LV-mEGFP:hCTNNA1_E307K | Gottardi Lab | VectorBuilder;CTNNA1 butterfly eye mutant |