Recombinant DNA
| Plasmid . | Source . | Identifier . |
|---|---|---|
| pENTR-LYST | This paper | NM_000081 |
| pENTR-ALFY | This paper | NM_014991 |
| pENTR-WDFY4 | This paper | NM_020945 |
| pENTR-LRBA | This paper | NM_006726 |
| pENTR223-NBEA | DF/HCC DNA Resource Core PlasmID Respository | PlasmID ID HsCD00399415, IMAGE:100069291 |
| pENTR-NBEAL1 | This paper | NM_001114132 |
| pENTR-NBEAL2 | This paper | NM_015175 |
| pENTR-NSMAF | This paper | NM_003580 |
| pDestFlpIn-3xFlag-EGFP-LYST | This paper | |
| pDestFlpIn-tdNGFlag-LYST | This paper | |
| pDestFlpIn-3xFlag-EGFP-ALFY | This paper | |
| pDestFlpIn-3xFlag-EGFP-WDFY4 | This paper | |
| pDestFlpIn-3xFlag-EGFP-LRBA | This paper | |
| pDestFlpIn-3xFlag-EGFP-NBEA | This paper | |
| pDestFlpIn-3xFlag-EGFP-NBEAL1 | This paper | |
| pDestFlpIn-3xFlag-EGFP-NBEAL2 | This paper | |
| pDestFlpIn-3xFlag-EGFP-NSMAF | This paper | |
| pcDNA5-FRT-TO-WDR81-EGFP-3xFlag | This paper | NM_001163809 |
| pDestFlpIn-3xFlag-EGFP-SUMOStar-LRBA | This paper | |
| pDestFlpIn-3xFlag-EGFP-SUMOStar-NBEAL2 | This paper | |
| pDestFlpIn-3xFlag-EGFP-NBEAL2(1-1792) | This paper | |
| pDestFlpIn-3xFlag-EGFP-NBEAL2(1792-2754) | This paper | |
| pLVX-SV40-mScarlet-I-RAB1A | This paper | |
| pLVX-SV40-mScarlet-I-RAB2A | This paper | |
| pLVX-SV40-mScarlet-I-RAB3A | This paper | |
| pLVX-SV40-mScarlet-I-RAB4A | This paper | |
| pLVX-SV40-mScarlet-I-RAB5A | This paper | |
| pLVX-SV40-mScarlet-I-RAB6A | This paper | |
| pLVX-SV40-mScarlet-I-RAB7A | This paper | |
| pLVX-SV40-mScarlet-I-RAB8A | This paper | |
| pLVX-SV40-mScarlet-I-RAB9A | This paper | |
| pLVX-SV40-mScarlet-I-RAB10 | This paper | |
| pLVX-SV40-mScarlet-I-RAB11A | This paper | |
| pLVX-SV40-mScarlet-I-RAB12 | This paper | |
| pLVX-SV40-mScarlet-I-RAB13 | This paper | |
| pLVX-SV40-mScarlet-I-RAB14 | This paper | |
| pLVX-SV40-mScarlet-I-RAB15 | This paper | |
| pLVX-SV40-mScarlet-I-RAB17 | This paper | |
| pLVX-SV40-mScarlet-I-RAB18 | This paper | |
| pLVX-SV40-mScarlet-I-RAB19 | This paper | |
| pLVX-SV40-mScarlet-I-RAB20 | This paper | |
| pLVX-SV40-mScarlet-I-RAB21 | This paper | |
| pLVX-SV40-mScarlet-I-RAB22A | This paper | |
| pLVX-SV40-mScarlet-I-RAB23 | This paper | |
| pLVX-SV40-mScarlet-I-RAB24 | This paper | |
| pLVX-SV40-mScarlet-I-RAB25 | This paper | |
| pLVX-SV40-mScarlet-I-RAB26 | This paper | |
| pLVX-SV40-mScarlet-I-RAB27A | This paper | |
| pLVX-SV40-mScarlet-I-RAB28 | This paper | |
| pLVX-SV40-mScarlet-I-RAB29 | This paper | |
| pLVX-SV40-mScarlet-I-RAB30 | This paper | |
| pLVX-SV40-mScarlet-I-RAB31 | This paper | |
| pLVX-SV40-mScarlet-I-RAB32 | This paper | |
| pLVX-SV40-mScarlet-I-RAB33B | This paper | |
| pLVX-SV40-mScarlet-I-RAB34 | This paper | |
| pLVX-SV40-mScarlet-I-RAB35 | This paper | |
| pLVX-SV40-mScarlet-I-RAB36 | This paper | |
| pLVX-SV40-mScarlet-I-RAB37 | This paper | |
| pLVX-SV40-mScarlet-I-RAB38 | This paper | |
| pLVX-SV40-mScarlet-I-RAB39A | This paper | |
| pLVX-SV40-mScarlet-I-RAB40A | This paper | |
| pLVX-SV40-mScarlet-I-RAB41 | This paper | |
| pLVX-SV40-mScarlet-I-RAB42 | This paper | |
| pLVX-SV40-mScarlet-I-RAB43 | This paper | |
| pLVX-SV40-ARF1-mScarlet-I | This paper | |
| pLVX-SV40-ARF3-mScarlet-I | This paper | |
| pLVX-SV40-ARF4-mScarlet-I | This paper | |
| pLVX-SV40-ARF5-mScarlet-I | This paper | |
| pLVX-SV40-ARF6-mScarlet-I | This paper | |
| pLVX-SV40-SAR1A-mScarlet-I | This paper | |
| pLVX-SV40-mScarlet-I-COPE | This paper | |
| pSBbi-pur-mScarlet-I-SEC31A | This paper | |
| pLVX-SV40-AP1M1-mScarlet-I | This paper | |
| pLVX-SV40-AP2M1-mScarlet-I | This paper | |
| pLVX-SV40-AP3M1-mScarlet-I | This paper | |
| pLVX-SV40-AP4M1-mScarlet-I | This paper | |
| pLVX-SV40-GGA1-mScarlet-I | This paper | |
| pLVX-SV40-GGA2-mScarlet-I | This paper | |
| pLVX-SV40-GGA3-mScarlet-I | This paper | |
| pLVX-SV40-CLINT1-mScarlet-I | This paper | |
| pSBbi-pur-mScarlet-I-CLTC | This paper | |
| pSBbi-pur-mScarlet-I-LRBA | This paper | |
| pSBbi-pur-mScarlet-I-NBEA | This paper | |
| Str-li-CTLA4-SBP-EGFP | This paper | |
| Str-li-CTLA4-SBP-mScarlet-I | This paper | |
| Str-li-CTLA4-SBP-mTagBFP2 | This paper | |
| pGEX-5X3 | GE Healthcare | Cat# 27-4586-01 |
| pDest15-CTLA4(187–223) | This paper | |
| pDest15-CTLA4(187–202) | This paper | |
| pDest15-CTLA4(194–209) | This paper | |
| pDest15-CTLA4(201–215) | This paper | |
| pDest15-CTLA4(208–223) | This paper | |
| pDest15-CTLA4(201–215)Y201A | This paper | |
| pDest15-CTLA4(201–215)V202A | This paper | |
| pDest15-CTLA4(201–215)K203A | This paper | |
| pDest15-CTLA4(201–215)M204A | This paper | |
| pDest15-CTLA4(201–215)P205A | This paper | |
| pDest15-CTLA4(201–215)P206A | This paper | |
| pDest15-CTLA4(201–215)T207A | This paper | |
| pDest15-CTLA4(201–215)E208A | This paper | |
| pDest15-CTLA4(201–215)P209A | This paper | |
| pDest15-CTLA4(201–215)E210A | This paper | |
| pDest15-CTLA4(201–215)C211A | This paper | |
| pDest15-CTLA4(201–215)E212A | This paper | |
| pDest15-CTLA4(201–215)K213A | This paper | |
| pDest15-CTLA4(201–215)Q214A | This paper | |
| pDest15-CTLA4(201–215)F215A | This paper | |
| pDest15-EPHA7(579–998) | This paper | |
| pDest15-EFNB2(251–333) | This paper | |
| pDest15-ROBO2(883–1378) | This paper | |
| pDest15-FGFR4(388–802) | This paper | |
| pDest15-FLRT2(563–660) | This paper | |
| pDest15-JAG1(1093–1218) | This paper | |
| pDest15-TGFBR3(810–851) | This paper | |
| pDest15-SDK1(2031–2213) | This paper | |
| pSpCas9(BB)-2A-Puro (PX459) V2.0 | Addgene | Cat#62988 |
| pX459-ALFYg1 | Søreng et al. (2022) | |
| pX459-ALFYg2 | Søreng et al. (2022) | |
| pX459-LRBAg1 | This paper | |
| pX459-LRBAg2 | This paper | |
| pX459-NBEAg1 | This paper | |
| pX459-NBEAg2 | This paper | |
| pX459-NBEAL1g1 | This paper | |
| pX459-NBEAL1g2 | This paper | |
| pX459-NBEAL2g1 | This paper | |
| pX459-NBEAL2g2 | This paper | |
| pCMV-VSV-G | Addgene | Cat#8454 |
| psPAX2 | Addgene | Cat#12260 |
| pOG44 | Invitrogen | Cat#V600520 |
| SB100X | Addgene | Cat# 127909 |
| pSBbi-pur | Addgene | Cat# 60523 |
| GFP-CHC17KDP | Addgene | Cat# 59799 |
| Str-Ii_VSVG-SBP-mCherry | Addgene | Cat# 65301 |
| pDest15 | Invitrogen | Cat# 11802014 |
| pEGFP-N1-hFGFR4 | Haugsten et al. (2014) |
| Plasmid . | Source . | Identifier . |
|---|---|---|
| pENTR-LYST | This paper | NM_000081 |
| pENTR-ALFY | This paper | NM_014991 |
| pENTR-WDFY4 | This paper | NM_020945 |
| pENTR-LRBA | This paper | NM_006726 |
| pENTR223-NBEA | DF/HCC DNA Resource Core PlasmID Respository | PlasmID ID HsCD00399415, IMAGE:100069291 |
| pENTR-NBEAL1 | This paper | NM_001114132 |
| pENTR-NBEAL2 | This paper | NM_015175 |
| pENTR-NSMAF | This paper | NM_003580 |
| pDestFlpIn-3xFlag-EGFP-LYST | This paper | |
| pDestFlpIn-tdNGFlag-LYST | This paper | |
| pDestFlpIn-3xFlag-EGFP-ALFY | This paper | |
| pDestFlpIn-3xFlag-EGFP-WDFY4 | This paper | |
| pDestFlpIn-3xFlag-EGFP-LRBA | This paper | |
| pDestFlpIn-3xFlag-EGFP-NBEA | This paper | |
| pDestFlpIn-3xFlag-EGFP-NBEAL1 | This paper | |
| pDestFlpIn-3xFlag-EGFP-NBEAL2 | This paper | |
| pDestFlpIn-3xFlag-EGFP-NSMAF | This paper | |
| pcDNA5-FRT-TO-WDR81-EGFP-3xFlag | This paper | NM_001163809 |
| pDestFlpIn-3xFlag-EGFP-SUMOStar-LRBA | This paper | |
| pDestFlpIn-3xFlag-EGFP-SUMOStar-NBEAL2 | This paper | |
| pDestFlpIn-3xFlag-EGFP-NBEAL2(1-1792) | This paper | |
| pDestFlpIn-3xFlag-EGFP-NBEAL2(1792-2754) | This paper | |
| pLVX-SV40-mScarlet-I-RAB1A | This paper | |
| pLVX-SV40-mScarlet-I-RAB2A | This paper | |
| pLVX-SV40-mScarlet-I-RAB3A | This paper | |
| pLVX-SV40-mScarlet-I-RAB4A | This paper | |
| pLVX-SV40-mScarlet-I-RAB5A | This paper | |
| pLVX-SV40-mScarlet-I-RAB6A | This paper | |
| pLVX-SV40-mScarlet-I-RAB7A | This paper | |
| pLVX-SV40-mScarlet-I-RAB8A | This paper | |
| pLVX-SV40-mScarlet-I-RAB9A | This paper | |
| pLVX-SV40-mScarlet-I-RAB10 | This paper | |
| pLVX-SV40-mScarlet-I-RAB11A | This paper | |
| pLVX-SV40-mScarlet-I-RAB12 | This paper | |
| pLVX-SV40-mScarlet-I-RAB13 | This paper | |
| pLVX-SV40-mScarlet-I-RAB14 | This paper | |
| pLVX-SV40-mScarlet-I-RAB15 | This paper | |
| pLVX-SV40-mScarlet-I-RAB17 | This paper | |
| pLVX-SV40-mScarlet-I-RAB18 | This paper | |
| pLVX-SV40-mScarlet-I-RAB19 | This paper | |
| pLVX-SV40-mScarlet-I-RAB20 | This paper | |
| pLVX-SV40-mScarlet-I-RAB21 | This paper | |
| pLVX-SV40-mScarlet-I-RAB22A | This paper | |
| pLVX-SV40-mScarlet-I-RAB23 | This paper | |
| pLVX-SV40-mScarlet-I-RAB24 | This paper | |
| pLVX-SV40-mScarlet-I-RAB25 | This paper | |
| pLVX-SV40-mScarlet-I-RAB26 | This paper | |
| pLVX-SV40-mScarlet-I-RAB27A | This paper | |
| pLVX-SV40-mScarlet-I-RAB28 | This paper | |
| pLVX-SV40-mScarlet-I-RAB29 | This paper | |
| pLVX-SV40-mScarlet-I-RAB30 | This paper | |
| pLVX-SV40-mScarlet-I-RAB31 | This paper | |
| pLVX-SV40-mScarlet-I-RAB32 | This paper | |
| pLVX-SV40-mScarlet-I-RAB33B | This paper | |
| pLVX-SV40-mScarlet-I-RAB34 | This paper | |
| pLVX-SV40-mScarlet-I-RAB35 | This paper | |
| pLVX-SV40-mScarlet-I-RAB36 | This paper | |
| pLVX-SV40-mScarlet-I-RAB37 | This paper | |
| pLVX-SV40-mScarlet-I-RAB38 | This paper | |
| pLVX-SV40-mScarlet-I-RAB39A | This paper | |
| pLVX-SV40-mScarlet-I-RAB40A | This paper | |
| pLVX-SV40-mScarlet-I-RAB41 | This paper | |
| pLVX-SV40-mScarlet-I-RAB42 | This paper | |
| pLVX-SV40-mScarlet-I-RAB43 | This paper | |
| pLVX-SV40-ARF1-mScarlet-I | This paper | |
| pLVX-SV40-ARF3-mScarlet-I | This paper | |
| pLVX-SV40-ARF4-mScarlet-I | This paper | |
| pLVX-SV40-ARF5-mScarlet-I | This paper | |
| pLVX-SV40-ARF6-mScarlet-I | This paper | |
| pLVX-SV40-SAR1A-mScarlet-I | This paper | |
| pLVX-SV40-mScarlet-I-COPE | This paper | |
| pSBbi-pur-mScarlet-I-SEC31A | This paper | |
| pLVX-SV40-AP1M1-mScarlet-I | This paper | |
| pLVX-SV40-AP2M1-mScarlet-I | This paper | |
| pLVX-SV40-AP3M1-mScarlet-I | This paper | |
| pLVX-SV40-AP4M1-mScarlet-I | This paper | |
| pLVX-SV40-GGA1-mScarlet-I | This paper | |
| pLVX-SV40-GGA2-mScarlet-I | This paper | |
| pLVX-SV40-GGA3-mScarlet-I | This paper | |
| pLVX-SV40-CLINT1-mScarlet-I | This paper | |
| pSBbi-pur-mScarlet-I-CLTC | This paper | |
| pSBbi-pur-mScarlet-I-LRBA | This paper | |
| pSBbi-pur-mScarlet-I-NBEA | This paper | |
| Str-li-CTLA4-SBP-EGFP | This paper | |
| Str-li-CTLA4-SBP-mScarlet-I | This paper | |
| Str-li-CTLA4-SBP-mTagBFP2 | This paper | |
| pGEX-5X3 | GE Healthcare | Cat# 27-4586-01 |
| pDest15-CTLA4(187–223) | This paper | |
| pDest15-CTLA4(187–202) | This paper | |
| pDest15-CTLA4(194–209) | This paper | |
| pDest15-CTLA4(201–215) | This paper | |
| pDest15-CTLA4(208–223) | This paper | |
| pDest15-CTLA4(201–215)Y201A | This paper | |
| pDest15-CTLA4(201–215)V202A | This paper | |
| pDest15-CTLA4(201–215)K203A | This paper | |
| pDest15-CTLA4(201–215)M204A | This paper | |
| pDest15-CTLA4(201–215)P205A | This paper | |
| pDest15-CTLA4(201–215)P206A | This paper | |
| pDest15-CTLA4(201–215)T207A | This paper | |
| pDest15-CTLA4(201–215)E208A | This paper | |
| pDest15-CTLA4(201–215)P209A | This paper | |
| pDest15-CTLA4(201–215)E210A | This paper | |
| pDest15-CTLA4(201–215)C211A | This paper | |
| pDest15-CTLA4(201–215)E212A | This paper | |
| pDest15-CTLA4(201–215)K213A | This paper | |
| pDest15-CTLA4(201–215)Q214A | This paper | |
| pDest15-CTLA4(201–215)F215A | This paper | |
| pDest15-EPHA7(579–998) | This paper | |
| pDest15-EFNB2(251–333) | This paper | |
| pDest15-ROBO2(883–1378) | This paper | |
| pDest15-FGFR4(388–802) | This paper | |
| pDest15-FLRT2(563–660) | This paper | |
| pDest15-JAG1(1093–1218) | This paper | |
| pDest15-TGFBR3(810–851) | This paper | |
| pDest15-SDK1(2031–2213) | This paper | |
| pSpCas9(BB)-2A-Puro (PX459) V2.0 | Addgene | Cat#62988 |
| pX459-ALFYg1 | Søreng et al. (2022) | |
| pX459-ALFYg2 | Søreng et al. (2022) | |
| pX459-LRBAg1 | This paper | |
| pX459-LRBAg2 | This paper | |
| pX459-NBEAg1 | This paper | |
| pX459-NBEAg2 | This paper | |
| pX459-NBEAL1g1 | This paper | |
| pX459-NBEAL1g2 | This paper | |
| pX459-NBEAL2g1 | This paper | |
| pX459-NBEAL2g2 | This paper | |
| pCMV-VSV-G | Addgene | Cat#8454 |
| psPAX2 | Addgene | Cat#12260 |
| pOG44 | Invitrogen | Cat#V600520 |
| SB100X | Addgene | Cat# 127909 |
| pSBbi-pur | Addgene | Cat# 60523 |
| GFP-CHC17KDP | Addgene | Cat# 59799 |
| Str-Ii_VSVG-SBP-mCherry | Addgene | Cat# 65301 |
| pDest15 | Invitrogen | Cat# 11802014 |
| pEGFP-N1-hFGFR4 | Haugsten et al. (2014) |
Plasmids and stable cell lines generated in this study are available from the lead contact.