Reagents and resources
| Reagent or resource . | Source . | Identifier . |
|---|---|---|
| Antibodies | ||
| Anti-EPS8 (mouse) (1:400) | Sigma-Aldrich | Cat# HPA003897; RRID: AB_1848224 |
| Anti-Eps8L2 (rabbit) (1:400) | Sigma-Aldrich | Cat# HPA041143; RRID: AB_10794985 |
| Anti-ZO-1 (rat) (1:100) | Milliporesigma | Cat# MABT11; RRID: AB_10616098 |
| Anti-NM2A (rabbit) (1:500) | BioLegend | Cat# 909802; RRID: AB_2734686 |
| Anti-γ-actin conjugated AF 647 (mouse) (1:100) | Santa Cruz Biotechnology | Cat# sc-65638 AF647; RRID: AB_2890621 |
| Anti-CAMSAP2 (rabbit) (1:200) | Novus | Cat# NBP1-21402; RRID: AB_1659977 |
| Anti-CAMSAP3 (rabbit) (1:100) | Thermo Fisher Scientific | Cat# PA5-48993; RRID: AB_2634449 |
| Anti-KIF1C (rabbit) | Cytoskeleton | Cat# AKIN11, RRID: AB_10707922 |
| Anti-myosin-5b (rabbit) (1:200) | Novus | Cat# NBP1-87746; RRID: AB_11034537 |
| Anti-myosin-7b (rabbit) (1:50) | Sigma-Aldrich | Cat# HPA039131; RRID: AB_10672771 |
| Anti-myosin-6 (rabbit) (1:100) | Thermo Fisher Scientific | Cat# PA5-57290, RRID: AB_2644375 |
| Anti-Girdin (Y1798) (rabbit) (1:100) | IBL—America | Cat# 28143, RRID: AB_3249611 |
| Anti-α-actinin 4 conjugated AF 647 (rabbit) (1:100) | Abcam | Abcam Cat# ab198610; RRID: AB_2890654 |
| Anti-fimbrin (mouse) (1:50) | Santa Cruz Biotechnology | Cat# sc-271223, RRID: AB_10614853 |
| Anti-Espin (rabbit) (1:500) | Thermo Fisher Scientific | Cat# PA5-5594; RRID: AB_2641126 |
| Anti-Advillin (rabbit) (1:500) | Thermo Fisher Scientific | Cat# PA5-63621; RRID: AB_2638403 |
| Anti-LIMA1 (rabbit) (1:500) | Sigma-Aldrich | Cat# HPA052645; RRID: AB_2681898 |
| Anti-myosin-1b (rabbit) (1:500) | Thermo Fisher Scientific | Cat# PA5-63820; RRID: AB_2644364 |
| Anti-Ezrin (rabbit) (1:200) | Cell Signaling | Cat# 3145S; RRID: AB_2100309 |
| Anti-pEzrin (T567) (rabbit) | Cell Signaling | Cat# 3726S; RRID: AB_10560513 |
| Anti-Ac-tubulin (mouse) (1:800) | Sigma-Aldrich | Cat# T6793; RRID: AB_477585 |
| Anti-dynein (mouse) (1:50) | Thermo Fisher Scientific | Cat# MA1-070; RRID: AB_2093668 |
| Anti-mCherry (rat) (1:100) | Thermo Fisher Scientific | Cat# M11217; RRID: AB_2536611 |
| Anti-pacsin-2 (1:200) | Sigma-Aldrich | Cat# HPA049854; RRID: AB_2680915 |
| Anti-dynamin-2 (1:200) | Sigma-Aldrich | Cat# HPA054246; RRID: AB_2682427 |
| Goat anti-mouse Alexa Fluor 488 F(ab′)2 fragment (1:1,000) | Thermo Fisher Scientific | Cat# A-11017; RRID: AB_2534084 |
| Goat anti-rabbit Alexa Fluor 488 F(ab′)2 fragment (1:1,000) | Thermo Fisher Scientific | Cat# A-11070; RRID: AB_2534114 |
| Goat anti-rabbit Alexa Fluor 568 F(ab′)2 fragment (1:1,000) | Thermo Fisher Scientific | Cat# A-21069; RRID: AB_2535730 |
| Chemicals, peptides, and recombinant proteins | ||
| Alexa Fluor Plus 405 Phalloidin (1:200) | Thermo Fisher Scientific | Cat# A30104 |
| Alexa Fluor 647 Phalloidin (1:200) | Thermo Fisher Scientific | Cat# A22287 |
| Alexa Fluor 568 Phalloidin (1:200) | Thermo Fisher Scientific | Cat# A12380 |
| 16% PFA | Electron Microscopy Sciences | Cat# 15710 |
| Triton X-100 | Sigma-Aldrich | Cat# T8787 |
| ProLong Gold Antifade Reagent | Invitrogen | Cat# P36930 |
| Lipofectamine 2000 | Thermo Fisher Scientific | Cat# 11668019 |
| Antibiotic-antimycotic (anti-anti) | Gibco | Cat# 15240062 |
| Glutaraldehyde 25% | Electron Microscopy Sciences | Cat# 16220 |
| Tannic acid | Electron Microscopy Sciences | Cat# 21700 |
| Osmium tetroxide | Electron Microscopy Sciences | Cat# 19112 |
| Cell lines | ||
| HeLa | American Type Culture Collection | Cat# CCL2 |
| Mouse models | ||
| C57BL6 | ||
| CDHR5-mCherry mice | Developed in-house by the Vanderbilt Genome Editing Resource | N/A |
| NM2C-EGFP mice | Generous gift from the Adelstein laboratory (NHLBI, NIH) as described in Ebrahim et al. (2013) | N/A |
| Recombinant DNA | ||
| EPS8L2-EGFP | Tyska laboratory | N/A |
| Software and algorithms | ||
| FIJI | https://fiji.sc/ | N/A |
| NIS AR Elements analysis | Nikon (https://www.microscope.healthcare.nikon.com/products/software/nis-elements/nis-elements-advanced-research) | N/A |
| Prism 9 | GraphPad (https://graphpad.com) | N/A |
| MATLAB | https://www.mathworks.com/products/matlab.html | N/A |
| Reagent or resource . | Source . | Identifier . |
|---|---|---|
| Antibodies | ||
| Anti-EPS8 (mouse) (1:400) | Sigma-Aldrich | Cat# HPA003897; RRID: AB_1848224 |
| Anti-Eps8L2 (rabbit) (1:400) | Sigma-Aldrich | Cat# HPA041143; RRID: AB_10794985 |
| Anti-ZO-1 (rat) (1:100) | Milliporesigma | Cat# MABT11; RRID: AB_10616098 |
| Anti-NM2A (rabbit) (1:500) | BioLegend | Cat# 909802; RRID: AB_2734686 |
| Anti-γ-actin conjugated AF 647 (mouse) (1:100) | Santa Cruz Biotechnology | Cat# sc-65638 AF647; RRID: AB_2890621 |
| Anti-CAMSAP2 (rabbit) (1:200) | Novus | Cat# NBP1-21402; RRID: AB_1659977 |
| Anti-CAMSAP3 (rabbit) (1:100) | Thermo Fisher Scientific | Cat# PA5-48993; RRID: AB_2634449 |
| Anti-KIF1C (rabbit) | Cytoskeleton | Cat# AKIN11, RRID: AB_10707922 |
| Anti-myosin-5b (rabbit) (1:200) | Novus | Cat# NBP1-87746; RRID: AB_11034537 |
| Anti-myosin-7b (rabbit) (1:50) | Sigma-Aldrich | Cat# HPA039131; RRID: AB_10672771 |
| Anti-myosin-6 (rabbit) (1:100) | Thermo Fisher Scientific | Cat# PA5-57290, RRID: AB_2644375 |
| Anti-Girdin (Y1798) (rabbit) (1:100) | IBL—America | Cat# 28143, RRID: AB_3249611 |
| Anti-α-actinin 4 conjugated AF 647 (rabbit) (1:100) | Abcam | Abcam Cat# ab198610; RRID: AB_2890654 |
| Anti-fimbrin (mouse) (1:50) | Santa Cruz Biotechnology | Cat# sc-271223, RRID: AB_10614853 |
| Anti-Espin (rabbit) (1:500) | Thermo Fisher Scientific | Cat# PA5-5594; RRID: AB_2641126 |
| Anti-Advillin (rabbit) (1:500) | Thermo Fisher Scientific | Cat# PA5-63621; RRID: AB_2638403 |
| Anti-LIMA1 (rabbit) (1:500) | Sigma-Aldrich | Cat# HPA052645; RRID: AB_2681898 |
| Anti-myosin-1b (rabbit) (1:500) | Thermo Fisher Scientific | Cat# PA5-63820; RRID: AB_2644364 |
| Anti-Ezrin (rabbit) (1:200) | Cell Signaling | Cat# 3145S; RRID: AB_2100309 |
| Anti-pEzrin (T567) (rabbit) | Cell Signaling | Cat# 3726S; RRID: AB_10560513 |
| Anti-Ac-tubulin (mouse) (1:800) | Sigma-Aldrich | Cat# T6793; RRID: AB_477585 |
| Anti-dynein (mouse) (1:50) | Thermo Fisher Scientific | Cat# MA1-070; RRID: AB_2093668 |
| Anti-mCherry (rat) (1:100) | Thermo Fisher Scientific | Cat# M11217; RRID: AB_2536611 |
| Anti-pacsin-2 (1:200) | Sigma-Aldrich | Cat# HPA049854; RRID: AB_2680915 |
| Anti-dynamin-2 (1:200) | Sigma-Aldrich | Cat# HPA054246; RRID: AB_2682427 |
| Goat anti-mouse Alexa Fluor 488 F(ab′)2 fragment (1:1,000) | Thermo Fisher Scientific | Cat# A-11017; RRID: AB_2534084 |
| Goat anti-rabbit Alexa Fluor 488 F(ab′)2 fragment (1:1,000) | Thermo Fisher Scientific | Cat# A-11070; RRID: AB_2534114 |
| Goat anti-rabbit Alexa Fluor 568 F(ab′)2 fragment (1:1,000) | Thermo Fisher Scientific | Cat# A-21069; RRID: AB_2535730 |
| Chemicals, peptides, and recombinant proteins | ||
| Alexa Fluor Plus 405 Phalloidin (1:200) | Thermo Fisher Scientific | Cat# A30104 |
| Alexa Fluor 647 Phalloidin (1:200) | Thermo Fisher Scientific | Cat# A22287 |
| Alexa Fluor 568 Phalloidin (1:200) | Thermo Fisher Scientific | Cat# A12380 |
| 16% PFA | Electron Microscopy Sciences | Cat# 15710 |
| Triton X-100 | Sigma-Aldrich | Cat# T8787 |
| ProLong Gold Antifade Reagent | Invitrogen | Cat# P36930 |
| Lipofectamine 2000 | Thermo Fisher Scientific | Cat# 11668019 |
| Antibiotic-antimycotic (anti-anti) | Gibco | Cat# 15240062 |
| Glutaraldehyde 25% | Electron Microscopy Sciences | Cat# 16220 |
| Tannic acid | Electron Microscopy Sciences | Cat# 21700 |
| Osmium tetroxide | Electron Microscopy Sciences | Cat# 19112 |
| Cell lines | ||
| HeLa | American Type Culture Collection | Cat# CCL2 |
| Mouse models | ||
| C57BL6 | ||
| CDHR5-mCherry mice | Developed in-house by the Vanderbilt Genome Editing Resource | N/A |
| NM2C-EGFP mice | Generous gift from the Adelstein laboratory (NHLBI, NIH) as described in Ebrahim et al. (2013) | N/A |
| Recombinant DNA | ||
| EPS8L2-EGFP | Tyska laboratory | N/A |
| Software and algorithms | ||
| FIJI | https://fiji.sc/ | N/A |
| NIS AR Elements analysis | Nikon (https://www.microscope.healthcare.nikon.com/products/software/nis-elements/nis-elements-advanced-research) | N/A |
| Prism 9 | GraphPad (https://graphpad.com) | N/A |
| MATLAB | https://www.mathworks.com/products/matlab.html | N/A |