Table 3.

Reagents used for construction of the osm-5p::mito-roGFP marker

Genomic osm-5p amplification (forward) 5′-CTG​ATC​ACG​GTA​CCG​CAT​GCC​TGC​AGG​AAA​TTG​AAA​GAC​TCG​AAC​CCC​ATA​ACT-3′ 
Genomic osm-5p amplification (reverse) 5′-GTA​CCA​ATA​CGA​CTC​ACT​ATA​GAG​CTC​TTT​GTA​TCA​TTA​AGA​AAA​GTG​TTC-3′ 
pOR823 amplification (forward) 5′-GAA​CAC​TTT​TCT​TAA​TGA​TAC​AAA​GAG​CTC​TAT​AGT​GAG​TCG​TAT​TGG​TAC-3′ 
pOR823 amplification (reverse) 5′-AGT​TAT​GGG​GTT​CGA​GTC​TTT​CAA​TTT​CCT​GCA​GGC​ATG​CGG​TAC​CGT​GAT​CAG-3′ 
Genomic osm-5p amplification (forward) 5′-CTG​ATC​ACG​GTA​CCG​CAT​GCC​TGC​AGG​AAA​TTG​AAA​GAC​TCG​AAC​CCC​ATA​ACT-3′ 
Genomic osm-5p amplification (reverse) 5′-GTA​CCA​ATA​CGA​CTC​ACT​ATA​GAG​CTC​TTT​GTA​TCA​TTA​AGA​AAA​GTG​TTC-3′ 
pOR823 amplification (forward) 5′-GAA​CAC​TTT​TCT​TAA​TGA​TAC​AAA​GAG​CTC​TAT​AGT​GAG​TCG​TAT​TGG​TAC-3′ 
pOR823 amplification (reverse) 5′-AGT​TAT​GGG​GTT​CGA​GTC​TTT​CAA​TTT​CCT​GCA​GGC​ATG​CGG​TAC​CGT​GAT​CAG-3′ 
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