Table 1.

Reagents used for generation of nekl-4(KD), nekl-4(PESTΔ), and pha-1 CRISPR mutations

NEKL-4(KD) ssODN 5′-CTC​ATA​AGC​TTT​TAT​AAT​CTT​TCA​GAT​GAT​GTT​AGC​CCT​TCG​CTA​TTT​GCA​CAA​AGA​AAA​GCA​AAT​TGT​ACA​TCG​AGA​CTT​AAA​GCC​AAA​TAA​CAT​AAT​GAT​GAC​GAC​AGA​CGA​GCG​AG-3′ 
NEKL-4(KD) crRNA 5′-UUC​UUU​GUG​CAA​AUA​GCG​AA-3′ 
PESTΔ ssODN 5′-TAC​TTC​AGA​TAT​GAC​TGC​TTC​ACA​ATC​TAG​TTA​TAA​TAT​CCG​TCC​TGG​AAG​GTC​GTC​AAA​GAC​ATC​AGG​GAG​TGG​AAA​G-3′ 
PESTΔ crRNA 5′-GUG​GAG​GAA​UUC​AGG​GAG​GA-3′ 
pha-1(e2123) ssODN 5′-GTA​ATC​AAA​ATA​CGA​ATC​GAA​GAC​TCA​AAA​AGA​GTA​TAC​TGT​ATG​ATT​ACA​GAT​GTT​CAT​CAA​GTT​ATT​CAT​AAA​TCA​TTG​ATA​GGT​TCA​G-3′ 
pha-1(e2123) crRNA 5′-AUG​UUC​AUC​AAG​UUA​UUC​AU-3′ 
NEKL-4(KD) ssODN 5′-CTC​ATA​AGC​TTT​TAT​AAT​CTT​TCA​GAT​GAT​GTT​AGC​CCT​TCG​CTA​TTT​GCA​CAA​AGA​AAA​GCA​AAT​TGT​ACA​TCG​AGA​CTT​AAA​GCC​AAA​TAA​CAT​AAT​GAT​GAC​GAC​AGA​CGA​GCG​AG-3′ 
NEKL-4(KD) crRNA 5′-UUC​UUU​GUG​CAA​AUA​GCG​AA-3′ 
PESTΔ ssODN 5′-TAC​TTC​AGA​TAT​GAC​TGC​TTC​ACA​ATC​TAG​TTA​TAA​TAT​CCG​TCC​TGG​AAG​GTC​GTC​AAA​GAC​ATC​AGG​GAG​TGG​AAA​G-3′ 
PESTΔ crRNA 5′-GUG​GAG​GAA​UUC​AGG​GAG​GA-3′ 
pha-1(e2123) ssODN 5′-GTA​ATC​AAA​ATA​CGA​ATC​GAA​GAC​TCA​AAA​AGA​GTA​TAC​TGT​ATG​ATT​ACA​GAT​GTT​CAT​CAA​GTT​ATT​CAT​AAA​TCA​TTG​ATA​GGT​TCA​G-3′ 
pha-1(e2123) crRNA 5′-AUG​UUC​AUC​AAG​UUA​UUC​AU-3′ 
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