Primary antibodies
| Antibody . | Source . | Species . | Concentration . | Notes . |
|---|---|---|---|---|
| Anti-GFP | Torrey Pines Scientific | Rabbit polyclonal | 1:300 or 1:100 for UExM | (Torrey Pines Biolabs Cat# TP401 071519, RRID:AB_10013661) |
| 12G10 (anti-alpha tubulin from Tetrahymena) | Developmental Studies Hybridoma Bank, University of Iowa | Mouse monoclonal | 1:250 or 1:100 for UExM | (DSHB Cat# 12G10 anti-alpha-tubulin, RRID:AB_1157911) |
| Anti-mCherry | Clontech | Mouse monoclonal | 1:800 | Not compatible with U-ExM; (Takara Bio Cat# 632543, RRID:AB_2307319) |
| Anti-mcherry | Encor Biotechnology | Rabbit polyclonal | 1:250 | (EnCor Biotechnology Cat# RPCA-mCherry, RRID:AB_2571870) |
| 6-11-B1 (Anti-acetylated tubulin) | EMD Millipore | Mouse monoclonal | 1:250 for UExM | (Sigma-Aldrich Cat# MABT868, RRID:AB_2819178) |
| Anti-TtCen1 | Kind gift from Alex Stemm-Wolf and Mark Winey | Rabbit polyclonal | 1:1,000 | (Stemm-Wolf et al., 2005) |
| Antibody . | Source . | Species . | Concentration . | Notes . |
|---|---|---|---|---|
| Anti-GFP | Torrey Pines Scientific | Rabbit polyclonal | 1:300 or 1:100 for UExM | (Torrey Pines Biolabs Cat# TP401 071519, RRID:AB_10013661) |
| 12G10 (anti-alpha tubulin from Tetrahymena) | Developmental Studies Hybridoma Bank, University of Iowa | Mouse monoclonal | 1:250 or 1:100 for UExM | (DSHB Cat# 12G10 anti-alpha-tubulin, RRID:AB_1157911) |
| Anti-mCherry | Clontech | Mouse monoclonal | 1:800 | Not compatible with U-ExM; (Takara Bio Cat# 632543, RRID:AB_2307319) |
| Anti-mcherry | Encor Biotechnology | Rabbit polyclonal | 1:250 | (EnCor Biotechnology Cat# RPCA-mCherry, RRID:AB_2571870) |
| 6-11-B1 (Anti-acetylated tubulin) | EMD Millipore | Mouse monoclonal | 1:250 for UExM | (Sigma-Aldrich Cat# MABT868, RRID:AB_2819178) |
| Anti-TtCen1 | Kind gift from Alex Stemm-Wolf and Mark Winey | Rabbit polyclonal | 1:1,000 | (Stemm-Wolf et al., 2005) |