Table 4.

Secondary antibodies (IF, immunofluorescence; WB, Western blot)

Antibody nameIdentifierUse
Alexa Fluor 488 AffiniPure Donkey Anti-Goat IgG (H + L) 705-545-003 IF 
Alexa Fluor 488 AffiniPure Donkey Anti-Chicken IgY (IgG) (H + L) 703-545-155 IF 
Alexa Fluor 647 AffiniPure Donkey Anti-Goat IgG (H + L) 705-605-147 IF 
Alexa Fluor 647 AffiniPure Donkey Anti-Guinea Pig IgG (H + L) 706-605-148 IF 
Alexa Fluor 488 AffiniPure Donkey Anti-Rabbit IgG (H + L) 711-545-152 IF 
Alexa Fluor 647 AffiniPure Donkey Anti-Rabbit IgG (H + L) 711-605-152 IF 
Alexa Fluor 647 AffiniPure Donkey Anti-Rat IgG (H + L) 712-605-150 IF 
Anti-rabbit IgG, HRP-linked Antibody #7074; Cell Signaling WB 
Rabbit Anti-Chicken IgG Antibody, HRP conjugate AP162P; Millipore Sigma WB 
Antibody nameIdentifierUse
Alexa Fluor 488 AffiniPure Donkey Anti-Goat IgG (H + L) 705-545-003 IF 
Alexa Fluor 488 AffiniPure Donkey Anti-Chicken IgY (IgG) (H + L) 703-545-155 IF 
Alexa Fluor 647 AffiniPure Donkey Anti-Goat IgG (H + L) 705-605-147 IF 
Alexa Fluor 647 AffiniPure Donkey Anti-Guinea Pig IgG (H + L) 706-605-148 IF 
Alexa Fluor 488 AffiniPure Donkey Anti-Rabbit IgG (H + L) 711-545-152 IF 
Alexa Fluor 647 AffiniPure Donkey Anti-Rabbit IgG (H + L) 711-605-152 IF 
Alexa Fluor 647 AffiniPure Donkey Anti-Rat IgG (H + L) 712-605-150 IF 
Anti-rabbit IgG, HRP-linked Antibody #7074; Cell Signaling WB 
Rabbit Anti-Chicken IgG Antibody, HRP conjugate AP162P; Millipore Sigma WB 

All IF secondary antibodies sourced from Jackson ImmunoResearch and used at 1:1,000. All WB antibodies were used at 1:5,000 dilution.

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