Primary antibodies and dilutions (IF, immunofluorescence; WB, Western blot)
| Antibody name . | Company . | Species . | Identifier . | Dilution . | Use . |
|---|---|---|---|---|---|
| α tubulin | Thermo Fisher Scientific | Rat | MA1-80017 | 1:1,000 | IF |
| β actin | Thermo Fisher Scientific | Rabbit | MA5-32540 | 1:1,000 | WB |
| β tubulin | Invitrogen | Rabbit | PA5-16863 | 1:1,000 | IF |
| 1:200 | WB | ||||
| Car4 | R&D Systems | Goat | AF2414 | 1:1,000 | IF |
| E-cadherin | Invitrogen | Rat | 13190 | 1:1,000 | IF |
| ERG | Abcam | Rabbit | AB92513 | 1:2500 | IF |
| GFP | Abcam | chicken | AB13970 | 1:5,000 | IF, WB |
| GFP | Invitrogen | Rabbit | G10362 | 1:500 | IF |
| Lamp1 | Development Studies Hybridoma Bank | Rat | 1D4B | 1:1,000 | IF |
| Lamp2 | Development Studies Hybridoma Bank | Rat | GL2A7 | 1:200 | IF |
| Lamp3 | Synaptic Systems | Guinea pig | 391005 | 1:500 | IF |
| Sox9 | R&D Systems | Goat | AF3075 | 1:1,000 | IF |
| tagRFP | Evrogen | Rabbit | ab233 | 1:500 | WB |
| Tomm20 | Genetex | Rabbit | GTX32928 | 1:500 | IF |
| Antibody name . | Company . | Species . | Identifier . | Dilution . | Use . |
|---|---|---|---|---|---|
| α tubulin | Thermo Fisher Scientific | Rat | MA1-80017 | 1:1,000 | IF |
| β actin | Thermo Fisher Scientific | Rabbit | MA5-32540 | 1:1,000 | WB |
| β tubulin | Invitrogen | Rabbit | PA5-16863 | 1:1,000 | IF |
| 1:200 | WB | ||||
| Car4 | R&D Systems | Goat | AF2414 | 1:1,000 | IF |
| E-cadherin | Invitrogen | Rat | 13190 | 1:1,000 | IF |
| ERG | Abcam | Rabbit | AB92513 | 1:2500 | IF |
| GFP | Abcam | chicken | AB13970 | 1:5,000 | IF, WB |
| GFP | Invitrogen | Rabbit | G10362 | 1:500 | IF |
| Lamp1 | Development Studies Hybridoma Bank | Rat | 1D4B | 1:1,000 | IF |
| Lamp2 | Development Studies Hybridoma Bank | Rat | GL2A7 | 1:200 | IF |
| Lamp3 | Synaptic Systems | Guinea pig | 391005 | 1:500 | IF |
| Sox9 | R&D Systems | Goat | AF3075 | 1:1,000 | IF |
| tagRFP | Evrogen | Rabbit | ab233 | 1:500 | WB |
| Tomm20 | Genetex | Rabbit | GTX32928 | 1:500 | IF |