Table 2.

Genotyping primers and expected sizes

NameSequenceExpected size (bp)
CAG F 5′-GGG​CGT​ACT​TGG​CAT​ATG​AT-3′ 212 
CAG R 5′-ATA​GCC​CAT​ATA​TGG​AGT​TC-3′ 
WPRE F 5′-CAC​GGG​GGA​GGG​GCA​AAC​AA-3′ 609 
WPRE R 5′-TGT​TGC​TCC​TTT​TAC​GCT​ATG-3′ 
BFP-Lamp1 F 5′-TAG​GTC​CCT​CGA​CCT​GCA​GC-3′ 453 
BFP Lamp1 R 5′-GCC​CTT​TGG​AGC​TGG​CAT​TG-3′ 
MLS-mKate2 F 5′-TGG​TGA​GCG​AGC​TGA​TTA​AG-3′ 676 
MLS-mKate2 R 5′-GGA​GGT​CGC​AGT​ATC​TGG​CC-3′ 
EGFP-αTubulin F 5′-GCA​TGG​ACG​AGC​TGT​ACA​AG-3′ 682 
EGFP-αTubulin R 5′-CTA​CGA​CAG​ATG​TCA​TAG​AT-3′ 
R26R F 5′-TTG​TTT​GCC​CCT​CCC​CCG​TG ∼4,500 
R26R R 5′-CCA​TTC​TCA​GTG​GCT​CAA​CA 
R26L F 5′-GGG​CGT​ACT​TGG​CAT​ATG​AT ∼1,500 
R26L R 5′-CGC​CTA​AAG​AAG​AGG​CTG​TG 
ROSAWT F 5′-GGA​GCG​GGA​GAA​ATG​GAT​ATG-3′ 426 
ROSAWT R 5′-CGT​GAT​CTG​CAA​CTC​CAG​TC-3′ 
complete insert F 5′-GCT​GAT​CCG​GAA​CCC​TTA​AT-3′ ∼6,000 
complete insert R 5′-CAT​AGC​GTA​AAA​GGA​GCA​ACA-3′ 
NameSequenceExpected size (bp)
CAG F 5′-GGG​CGT​ACT​TGG​CAT​ATG​AT-3′ 212 
CAG R 5′-ATA​GCC​CAT​ATA​TGG​AGT​TC-3′ 
WPRE F 5′-CAC​GGG​GGA​GGG​GCA​AAC​AA-3′ 609 
WPRE R 5′-TGT​TGC​TCC​TTT​TAC​GCT​ATG-3′ 
BFP-Lamp1 F 5′-TAG​GTC​CCT​CGA​CCT​GCA​GC-3′ 453 
BFP Lamp1 R 5′-GCC​CTT​TGG​AGC​TGG​CAT​TG-3′ 
MLS-mKate2 F 5′-TGG​TGA​GCG​AGC​TGA​TTA​AG-3′ 676 
MLS-mKate2 R 5′-GGA​GGT​CGC​AGT​ATC​TGG​CC-3′ 
EGFP-αTubulin F 5′-GCA​TGG​ACG​AGC​TGT​ACA​AG-3′ 682 
EGFP-αTubulin R 5′-CTA​CGA​CAG​ATG​TCA​TAG​AT-3′ 
R26R F 5′-TTG​TTT​GCC​CCT​CCC​CCG​TG ∼4,500 
R26R R 5′-CCA​TTC​TCA​GTG​GCT​CAA​CA 
R26L F 5′-GGG​CGT​ACT​TGG​CAT​ATG​AT ∼1,500 
R26L R 5′-CGC​CTA​AAG​AAG​AGG​CTG​TG 
ROSAWT F 5′-GGA​GCG​GGA​GAA​ATG​GAT​ATG-3′ 426 
ROSAWT R 5′-CGT​GAT​CTG​CAA​CTC​CAG​TC-3′ 
complete insert F 5′-GCT​GAT​CCG​GAA​CCC​TTA​AT-3′ ∼6,000 
complete insert R 5′-CAT​AGC​GTA​AAA​GGA​GCA​ACA-3′ 
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