Cloning primers
| Cloning primers (cut sites in bold) . | Location . | Sites . | Order . |
|---|---|---|---|
| 5′-ATCATCCCCGGGCGCGCCGGCTAGAAGATGGG-3′ | R26 Short 5′ | XmaI | 1 |
| 5′-ACTACTCTCGAGAAACAAATAGGATACTAGAA-3′ | R26 Short 3′ | XhoI | 1 |
| 5′-ATCATCAGATCTACGCGTGCCACCATGGCGGCCCCGGG-3′ | BFP-Lysosomes F | BglII; MluI | 2 |
| 5′-ACTACTGCTAGCATTAAGCTTGTGCCCCAGTT-3′ | BFP-Lysosomes R | NheI | 2 |
| 5′-ATCATCACTAGTATGTCCGTCCTGACGCCGCT-3′ | MLS-mKate2 F | SpeI | 4 |
| 5′-ACTACTAAGCTTTCTGTGCCCCAGTTTGCTAG-3′ | MLS-mKate2 R | HindIII | 4 |
| 5′-ATCATCGTCGACATGGTGAGCAAGGGCGAGGA-3′ | EGFP-Tubulin F | SalI | 3 |
| 5′-ACTACTTCTAGAACGCGTTTAGTATTCCTCTCCTTCTTCCT-3′ | EGFP-Tubulin R | MluI; XbaI | 3 |
| Cloning primers (cut sites in bold) . | Location . | Sites . | Order . |
|---|---|---|---|
| 5′-ATCATCCCCGGGCGCGCCGGCTAGAAGATGGG-3′ | R26 Short 5′ | XmaI | 1 |
| 5′-ACTACTCTCGAGAAACAAATAGGATACTAGAA-3′ | R26 Short 3′ | XhoI | 1 |
| 5′-ATCATCAGATCTACGCGTGCCACCATGGCGGCCCCGGG-3′ | BFP-Lysosomes F | BglII; MluI | 2 |
| 5′-ACTACTGCTAGCATTAAGCTTGTGCCCCAGTT-3′ | BFP-Lysosomes R | NheI | 2 |
| 5′-ATCATCACTAGTATGTCCGTCCTGACGCCGCT-3′ | MLS-mKate2 F | SpeI | 4 |
| 5′-ACTACTAAGCTTTCTGTGCCCCAGTTTGCTAG-3′ | MLS-mKate2 R | HindIII | 4 |
| 5′-ATCATCGTCGACATGGTGAGCAAGGGCGAGGA-3′ | EGFP-Tubulin F | SalI | 3 |
| 5′-ACTACTTCTAGAACGCGTTTAGTATTCCTCTCCTTCTTCCT-3′ | EGFP-Tubulin R | MluI; XbaI | 3 |