Primary antibodies used for samples for confocal imaging
| Antibody . | Host . | Dilution for microscopy . | Supplier . | Catalog nr. . | Notes . |
|---|---|---|---|---|---|
| ALG-2 | Rabbit | 1:200 | Proteintech | 12303-1-AP | |
| ALIX | Mouse | 1:100 | BioLegend | 634502 | |
| CABIN1 | Rabbit | 1:100 | NovusBio | NBP1-91745 | |
| CD63 | Mouse | 1:200 | Santa Cruz | sc-59284 | |
| CD9 | Rabbit | 1:100 | Abcam | ab97999 | |
| Calprotectin heterodimer | Rabbit | 1:100 | R&D systems | MAB45701-SP | |
| CHMP1B | Mouse | 1:50 | Santa Cruz | sc-514013 | Used for CLSM, STED, and TEM |
| CHMP4A | Rabbit | 1:100 | Thermo Fisher Scientific | PA5-49410 | |
| Clathrin light chain | Mouse | 1:500 | Sigma-Aldrich | C1985 | |
| DAB2 | Rabbit | 1:100 | CST | 129065 | |
| EEA1 | Mouse | 1:100 | BD Biosciences | 610456/610457 | |
| ERGIC53 | Mouse | 1:200 | Enzo Life Sciences | enz-ABS300-0100 | |
| GFP | Rabbit | 1:200 | Abcam | ab6556 | |
| GM130 | Mouse | 1:200 | BD | 610822 | |
| Histone H3 | Rabbit | 1:200 | Abcam | ab5103 | Methanol fixation |
| HLA-DP/DQ/DR | Mouse | 1:100 | NSJ Bio | V3374 | |
| HLA-DR | Mouse | 1:100 | BioLegend | 307614 | |
| HLA-ABC | Rabbit | 1:200 | Thermo Fisher Scientific | 14-9983-82 | |
| HNRNPC | Rabbit | 1:200 | Abcam | ab133607 | |
| Integrin β1 | Rat | 1:200 | Gift of Carl Figdor | Clone: AIIB2 | |
| Integrin β2 | Mouse | 1:200 | Gift of Carl Figdor | Clone: L19 | |
| Integrin αVβ5 | Mouse | 1:200 | Merck Millipore | Clone 15F11 | |
| Integrin αM | Rat | 1:200 | BioLegend | 101204 | |
| IST1 | Mouse | 1:100 | Proteintech | 66989-1-lg | Methanol fixation |
| IST1 | Rabbit | 1:1,000 | Bethyl | A305-411A | |
| Lamin A/C | Rabbit | 1:200 | Abcam | ab108595 | |
| LAMP1 | Mouse | 1:200 | BioLegend | 328601 | |
| LC3 | Rabbit | 1:100 | CST | 2775S | |
| NUMB | Rabbit | 1:100 | CST | 2756S | |
| PDI | Rabbit | 1:100 | Novus Bio | NB300-517 | |
| S100a8 | Mouse | 1:200 | Proteintech | 66853-1-Ig | |
| S100a9 | Rabbit | 1:200 | Thermo Fisher Scientific | PA1-46489 | |
| Sec22b | Rabbit | 1:100 | Novus Biologicals | NB100-91277 | |
| Spastin | Rabbit | 1:100 | Novus Biological | NBP1-90230 | |
| Talin | Mouse | 1:100 | Sigma-Aldrich | T3287 | |
| TAPBP | Rabbit | 1:100 | LS Bio | LS-C331792 | |
| TOMM20 | Mouse | 1:100 | Abcam | ab56783 | |
| TSG101 | Mouse | 1:50 | BD | BD61296 | |
| Tubulin | Rat | 1:500 | Novus Biologicals | NB100-1639 | Used for CLSM and STED |
| Ubiquitin | Mouse | 1:200 | Enzo Life Sciences | BML-PW8810-100 | |
| Vinculin | Mouse | 1:300 | Sigma-Aldrich | V9131 | |
| VPS4 | Rabbit | 1:100 | Abcam | ab180581 | In some cases this labeled the cytoskeleton. |
| Antibody . | Host . | Dilution for microscopy . | Supplier . | Catalog nr. . | Notes . |
|---|---|---|---|---|---|
| ALG-2 | Rabbit | 1:200 | Proteintech | 12303-1-AP | |
| ALIX | Mouse | 1:100 | BioLegend | 634502 | |
| CABIN1 | Rabbit | 1:100 | NovusBio | NBP1-91745 | |
| CD63 | Mouse | 1:200 | Santa Cruz | sc-59284 | |
| CD9 | Rabbit | 1:100 | Abcam | ab97999 | |
| Calprotectin heterodimer | Rabbit | 1:100 | R&D systems | MAB45701-SP | |
| CHMP1B | Mouse | 1:50 | Santa Cruz | sc-514013 | Used for CLSM, STED, and TEM |
| CHMP4A | Rabbit | 1:100 | Thermo Fisher Scientific | PA5-49410 | |
| Clathrin light chain | Mouse | 1:500 | Sigma-Aldrich | C1985 | |
| DAB2 | Rabbit | 1:100 | CST | 129065 | |
| EEA1 | Mouse | 1:100 | BD Biosciences | 610456/610457 | |
| ERGIC53 | Mouse | 1:200 | Enzo Life Sciences | enz-ABS300-0100 | |
| GFP | Rabbit | 1:200 | Abcam | ab6556 | |
| GM130 | Mouse | 1:200 | BD | 610822 | |
| Histone H3 | Rabbit | 1:200 | Abcam | ab5103 | Methanol fixation |
| HLA-DP/DQ/DR | Mouse | 1:100 | NSJ Bio | V3374 | |
| HLA-DR | Mouse | 1:100 | BioLegend | 307614 | |
| HLA-ABC | Rabbit | 1:200 | Thermo Fisher Scientific | 14-9983-82 | |
| HNRNPC | Rabbit | 1:200 | Abcam | ab133607 | |
| Integrin β1 | Rat | 1:200 | Gift of Carl Figdor | Clone: AIIB2 | |
| Integrin β2 | Mouse | 1:200 | Gift of Carl Figdor | Clone: L19 | |
| Integrin αVβ5 | Mouse | 1:200 | Merck Millipore | Clone 15F11 | |
| Integrin αM | Rat | 1:200 | BioLegend | 101204 | |
| IST1 | Mouse | 1:100 | Proteintech | 66989-1-lg | Methanol fixation |
| IST1 | Rabbit | 1:1,000 | Bethyl | A305-411A | |
| Lamin A/C | Rabbit | 1:200 | Abcam | ab108595 | |
| LAMP1 | Mouse | 1:200 | BioLegend | 328601 | |
| LC3 | Rabbit | 1:100 | CST | 2775S | |
| NUMB | Rabbit | 1:100 | CST | 2756S | |
| PDI | Rabbit | 1:100 | Novus Bio | NB300-517 | |
| S100a8 | Mouse | 1:200 | Proteintech | 66853-1-Ig | |
| S100a9 | Rabbit | 1:200 | Thermo Fisher Scientific | PA1-46489 | |
| Sec22b | Rabbit | 1:100 | Novus Biologicals | NB100-91277 | |
| Spastin | Rabbit | 1:100 | Novus Biological | NBP1-90230 | |
| Talin | Mouse | 1:100 | Sigma-Aldrich | T3287 | |
| TAPBP | Rabbit | 1:100 | LS Bio | LS-C331792 | |
| TOMM20 | Mouse | 1:100 | Abcam | ab56783 | |
| TSG101 | Mouse | 1:50 | BD | BD61296 | |
| Tubulin | Rat | 1:500 | Novus Biologicals | NB100-1639 | Used for CLSM and STED |
| Ubiquitin | Mouse | 1:200 | Enzo Life Sciences | BML-PW8810-100 | |
| Vinculin | Mouse | 1:300 | Sigma-Aldrich | V9131 | |
| VPS4 | Rabbit | 1:100 | Abcam | ab180581 | In some cases this labeled the cytoskeleton. |