Triple fluorescence staining panel used for immunohistochemistry
| . | Targets . | ||||
|---|---|---|---|---|---|
| Vimentin | IST1 | CD68 | Nucleus | ||
| Isotype (primary ab) | Rabbit polyclonal (ab92547; Abcam) (1:250 dilution) | Mouse IgG2a (66989-1-Ig; Proteintech) (1:750 dilution) | Mouse IgG3 (M0876; DAKO) (1:100 dilution) | ||
| Secondary ab | Donkey anti-rabbit IgG (ab150075; Abcam) (1:50 dilution) | Rat anti-mouse IgG2a (RMG2a-62; BioLegend) (1:20 dilution) | Goat anti-mouse IgG3 (1101-01; Southern bio) (1:75 dilution) | ||
| Tertiary ab | Donkey anti-rat IgG (ab150153; Abcam) (1:40 dilution) | Donkey anti-goat IgG (ab175704; Abcam) (1:75 dilution) | |||
| Conjugate/dye | AF647 | AF488 | AF568 | DAPI | |
| Longpass filter | 610LP | 515LP | 590LP | 420LP | |
| Targets | |||||
| CD18 | IST1 | CD68 | Nucleus | ||
| Isotype (primary ab) | Rabbit polyclonal (a2173; Abclonal) (1:150 dilution) | Mouse IgG2a (66989-1-Ig; Proteintech) (1:750 dilution) | Mouse IgG3 (M0876; DAKO) (1:100 dilution) | ||
| Secondary ab | Donkey anti-rabbit IgG (ab150075; Abcam) (1:50 dilution) | Rat anti-mouse IgG2a (RMG2a-62; BioLegend) (1:20 dilution) | Goat anti-mouse IgG3 (1101-01; Southern bio) (1:100 dilution) | ||
| Tertiary ab | Donkey anti-rat IgG (ab150153; Abcam) (1:40 dilution) | Donkey anti-goat IgG (ab175704; Abcam) (1:100 dilution) | |||
| Conjugate/dye | AF647 | AF488 | AF568 | DAPI | |
| Longpass filter | 610LP | 515LP | 590LP | 420LP | |
| Targets | |||||
| CD18 | IST1 | Vimentin | Nucleus | ||
| Isotype (primary ab) | Rabbit polyclonal (a2173; Abclonal) (1:150 dilution) | Mouse IgG2a (66989-1-Ig; Proteintech) (1:750 dilution) | Mouse IgG1 (RV202; Santa Cruz) (1:300 dilution) | ||
| Secondary ab | Donkey anti-rabbit IgG (ab150075; Abcam) (1:50 dilution) | Rat anti-mouse IgG2a (RMG2a-62; BioLegend) (1:20 dilution) | Goat anti-mouse IgG1 (1071-01; Southern bio) (1:100 dilution) | ||
| Tertiary ab | Donkey anti-rat IgG (ab150153; Abcam) (1:40 dilution) | Donkey anti-goat IgG (ab175704; Abcam) (1:100 dilution) | |||
| Conjugate/dye | AF647 | AF488 | AF568 | DAPI | |
| Longpass filter | 610LP | 515LP | 590LP | 420LP | |
| . | Targets . | ||||
|---|---|---|---|---|---|
| Vimentin | IST1 | CD68 | Nucleus | ||
| Isotype (primary ab) | Rabbit polyclonal (ab92547; Abcam) (1:250 dilution) | Mouse IgG2a (66989-1-Ig; Proteintech) (1:750 dilution) | Mouse IgG3 (M0876; DAKO) (1:100 dilution) | ||
| Secondary ab | Donkey anti-rabbit IgG (ab150075; Abcam) (1:50 dilution) | Rat anti-mouse IgG2a (RMG2a-62; BioLegend) (1:20 dilution) | Goat anti-mouse IgG3 (1101-01; Southern bio) (1:75 dilution) | ||
| Tertiary ab | Donkey anti-rat IgG (ab150153; Abcam) (1:40 dilution) | Donkey anti-goat IgG (ab175704; Abcam) (1:75 dilution) | |||
| Conjugate/dye | AF647 | AF488 | AF568 | DAPI | |
| Longpass filter | 610LP | 515LP | 590LP | 420LP | |
| Targets | |||||
| CD18 | IST1 | CD68 | Nucleus | ||
| Isotype (primary ab) | Rabbit polyclonal (a2173; Abclonal) (1:150 dilution) | Mouse IgG2a (66989-1-Ig; Proteintech) (1:750 dilution) | Mouse IgG3 (M0876; DAKO) (1:100 dilution) | ||
| Secondary ab | Donkey anti-rabbit IgG (ab150075; Abcam) (1:50 dilution) | Rat anti-mouse IgG2a (RMG2a-62; BioLegend) (1:20 dilution) | Goat anti-mouse IgG3 (1101-01; Southern bio) (1:100 dilution) | ||
| Tertiary ab | Donkey anti-rat IgG (ab150153; Abcam) (1:40 dilution) | Donkey anti-goat IgG (ab175704; Abcam) (1:100 dilution) | |||
| Conjugate/dye | AF647 | AF488 | AF568 | DAPI | |
| Longpass filter | 610LP | 515LP | 590LP | 420LP | |
| Targets | |||||
| CD18 | IST1 | Vimentin | Nucleus | ||
| Isotype (primary ab) | Rabbit polyclonal (a2173; Abclonal) (1:150 dilution) | Mouse IgG2a (66989-1-Ig; Proteintech) (1:750 dilution) | Mouse IgG1 (RV202; Santa Cruz) (1:300 dilution) | ||
| Secondary ab | Donkey anti-rabbit IgG (ab150075; Abcam) (1:50 dilution) | Rat anti-mouse IgG2a (RMG2a-62; BioLegend) (1:20 dilution) | Goat anti-mouse IgG1 (1071-01; Southern bio) (1:100 dilution) | ||
| Tertiary ab | Donkey anti-rat IgG (ab150153; Abcam) (1:40 dilution) | Donkey anti-goat IgG (ab175704; Abcam) (1:100 dilution) | |||
| Conjugate/dye | AF647 | AF488 | AF568 | DAPI | |
| Longpass filter | 610LP | 515LP | 590LP | 420LP | |