Table 3.

FLAT element sequences for luciferase reporter assays

NameSequenceSource
Pro-insulin FLAT 5′-CTA​GTT​TAA​TAA​TCT​AAT​TAC​GGA​TCC​TTA​ATA​ATC​TAA​TTA​CAC​GTG​ATT​AAT​AAT​CTA​ATT​ACC-3′/5′-TCG​AGG​TAA​TTA​GAT​TAT​TAA​TCA​CGT​GTA​ATT​AGA​TTA​TTA​AGG​ATC​CGT​AAT​TAG​ATT​ATT​AAA-3′ Eurofins 
NURR1 FLAT 5′-CTA​GTA​AAA​TAA​TAA​CTG​CGG​ATC​CAA​AAT​AAT​AAC​TGC​ACG​TGA​AAA​ATA​ATA​ACT​GCC-3′/5′-TCG​AGG​CAG​TTA​TTA​TTT​TTC​ACG​TGC​AGT​TAT​TAT​TTT​GGA​TCC​GCA​GTT​ATT​ATT​TTA-3′ Eurofins 
ULK1 FLAT 5′-CTA​GTG​ATT​ATA​ATT​AAG​TAG​GAT​CCG​ATT​ATA​ATT​AAG​TAA​CGT​GAG​ATT​ATA​ATT​AAG​TAC-3′/5′-TCG​AGT​ACT​TAA​TTA​TAA​TCT​CAC​GTT​ACT​TAA​TTA​TAA​TCG​GAT​CCT​ACT​TAA​TTA​TAA​TCA-3′ Eurofins 
TFEB FLAT 5′-CTA​GTG​TGC​AGG​TTA​ATT​ACT​GCC​CAG​GAT​CCG​TGC​AGG​TTA​ATT​ACT​GCC​CAA​CGT​GAG​TGC​AGG​TTA​ATT​ACT​GCC​CAC-3′/5′-TCG​AGT​GGG​CAG​TAA​TTA​ACC​TGC​ACT​CAC​GTT​GGG​CAG​TAA​TTA​ACC​TGC​ACG​GAT​CCT​GGG​CAG​TAA​TTA​ACC​TGC​ACA-3′ Eurofins 
PINK1 FLAT 5′-CTA​GTG​CTG​TTA​AAT​AAA​TTA​AAA​GAC​GGA​TCC​GCT​GTT​AAA​TAA​ATT​AAA​AGA​CAC​GTG​AGC​TGT​TAA​ATA​AAT​TAA​AAG​ACC-3′/5′-TCG​AGG​TCT​TTT​AAT​TTA​TTT​AAC​AGC​TCA​CGT​GTC​TTT​TAA​TTT​ATT​TAA​CAG​CGG​ATC​CGT​CTT​TTA​ATT​TAT​TTA​ACA​GCA-3′ Eurofins 
UVRAG FLAT 5′-CTA​GTC​ATC​CTT​TTA​ATG​AAA​ATC​ATA​TGG​ATC​CCA​TCC​TTT​TAA​TGA​AAA​TCA​TAT​ACG​TGA​CAT​CCT​TTT​AAT​GAA​AAT​CAT​ATC-3′/5′-TCG​AGA​TAT​GAT​TTT​CAT​TAA​AAG​GAT​GTC​ACG​TAT​ATG​ATT​TTC​ATT​AAA​AGG​ATG​GGA​TCC​ATA​TGA​TTT​TCA​TTA​AAA​GGA​TGA-3′ Eurofins 
Scrambled control 5′-CTA​GTT​CAG​ATT​CAC​GGG​ATC​CTC​AGA​TTC​ACG​GTC​GAC​TCA​GAT​TCA​CGC-3′/5′-TCG​AGC​GTG​AAT​CTG​AGT​CGA​CCG​TGA​ATC​TGA​GGA​TCC​CGT​GAA​TCT​GAA-3′ Eurofins 
NameSequenceSource
Pro-insulin FLAT 5′-CTA​GTT​TAA​TAA​TCT​AAT​TAC​GGA​TCC​TTA​ATA​ATC​TAA​TTA​CAC​GTG​ATT​AAT​AAT​CTA​ATT​ACC-3′/5′-TCG​AGG​TAA​TTA​GAT​TAT​TAA​TCA​CGT​GTA​ATT​AGA​TTA​TTA​AGG​ATC​CGT​AAT​TAG​ATT​ATT​AAA-3′ Eurofins 
NURR1 FLAT 5′-CTA​GTA​AAA​TAA​TAA​CTG​CGG​ATC​CAA​AAT​AAT​AAC​TGC​ACG​TGA​AAA​ATA​ATA​ACT​GCC-3′/5′-TCG​AGG​CAG​TTA​TTA​TTT​TTC​ACG​TGC​AGT​TAT​TAT​TTT​GGA​TCC​GCA​GTT​ATT​ATT​TTA-3′ Eurofins 
ULK1 FLAT 5′-CTA​GTG​ATT​ATA​ATT​AAG​TAG​GAT​CCG​ATT​ATA​ATT​AAG​TAA​CGT​GAG​ATT​ATA​ATT​AAG​TAC-3′/5′-TCG​AGT​ACT​TAA​TTA​TAA​TCT​CAC​GTT​ACT​TAA​TTA​TAA​TCG​GAT​CCT​ACT​TAA​TTA​TAA​TCA-3′ Eurofins 
TFEB FLAT 5′-CTA​GTG​TGC​AGG​TTA​ATT​ACT​GCC​CAG​GAT​CCG​TGC​AGG​TTA​ATT​ACT​GCC​CAA​CGT​GAG​TGC​AGG​TTA​ATT​ACT​GCC​CAC-3′/5′-TCG​AGT​GGG​CAG​TAA​TTA​ACC​TGC​ACT​CAC​GTT​GGG​CAG​TAA​TTA​ACC​TGC​ACG​GAT​CCT​GGG​CAG​TAA​TTA​ACC​TGC​ACA-3′ Eurofins 
PINK1 FLAT 5′-CTA​GTG​CTG​TTA​AAT​AAA​TTA​AAA​GAC​GGA​TCC​GCT​GTT​AAA​TAA​ATT​AAA​AGA​CAC​GTG​AGC​TGT​TAA​ATA​AAT​TAA​AAG​ACC-3′/5′-TCG​AGG​TCT​TTT​AAT​TTA​TTT​AAC​AGC​TCA​CGT​GTC​TTT​TAA​TTT​ATT​TAA​CAG​CGG​ATC​CGT​CTT​TTA​ATT​TAT​TTA​ACA​GCA-3′ Eurofins 
UVRAG FLAT 5′-CTA​GTC​ATC​CTT​TTA​ATG​AAA​ATC​ATA​TGG​ATC​CCA​TCC​TTT​TAA​TGA​AAA​TCA​TAT​ACG​TGA​CAT​CCT​TTT​AAT​GAA​AAT​CAT​ATC-3′/5′-TCG​AGA​TAT​GAT​TTT​CAT​TAA​AAG​GAT​GTC​ACG​TAT​ATG​ATT​TTC​ATT​AAA​AGG​ATG​GGA​TCC​ATA​TGA​TTT​TCA​TTA​AAA​GGA​TGA-3′ Eurofins 
Scrambled control 5′-CTA​GTT​CAG​ATT​CAC​GGG​ATC​CTC​AGA​TTC​ACG​GTC​GAC​TCA​GAT​TCA​CGC-3′/5′-TCG​AGC​GTG​AAT​CTG​AGT​CGA​CCG​TGA​ATC​TGA​GGA​TCC​CGT​GAA​TCT​GAA-3′ Eurofins 
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