Table 1.

Primers for mutagenesis and deletion constructs

NameSequenceSource
Human LMX1BY309A/L312A 5′-CAT​GAT​GGC​TTC​CGC​CAC​GCC​GGC​GGC​CCC​ACC​ACA​G-3′/5′-CTG​TGG​TGG​GGC​CGC​CGG​CGT​GGC​GGA​AGC​CAT​CAT​G-3′ Eurofins 
Human LMX1BY309A/L312A construct 5′-GAA​TGA​TGG​CAT​CCG​CCA​CCC​CAC​TTG​CAC​CGC​CAC​AAC​AGC-3′/5′-GCT​GTT​GTG​GCG​GTG​CAA​GTG​GGG​TGG​CGG​ATG​CCA​TCA​TTC-3′ Eurofins 
Human LMX1B Δ308-317 5′-CAG​ATC​GTG​GCC​ATG​GAA​CAG-3′/5′-AGC​CAT​CAT​GCC​CTC​CAT​GC-3′ Eurofins 
Human LMX1B Δ308-317 construct 5′-CAG​ATT​GTG​GCC​ATG​GAG​CAG​TCA​CCG​TA-3′/5′-TGC​CAT​CAT​TCC​CTC​CAT​TCT​AGA​GGA​CAG​G-3′ Eurofins 
Human LMX1AY290A/L293A 5′-GGA​AGG​TAT​CAT​GAA​TCC​GGC​TAC​AGC​GGC​GCC​AAC​ACC​ACA​GCA​GTT​GC-3′/5′-GCA​ACT​GCT​GTG​GTG​TTG​GCG​CCG​CTG​TAG​CCG​GAT​TCA​TGA​TAC​CTT​CC-3′ Eurofins 
Human LC3BF52A/L53A 5′-GTT​CTG​GAT​AAA​ACA​AAG​GCC​GCT​GTA​CCT​GAC​CAT​GTC-3′/5′-GAC​ATG​GTC​AGG​TAC​AGC​GGC​CTT​TGT​TTT​ATC​CAG​AAC-3′ Eurofins 
NameSequenceSource
Human LMX1BY309A/L312A 5′-CAT​GAT​GGC​TTC​CGC​CAC​GCC​GGC​GGC​CCC​ACC​ACA​G-3′/5′-CTG​TGG​TGG​GGC​CGC​CGG​CGT​GGC​GGA​AGC​CAT​CAT​G-3′ Eurofins 
Human LMX1BY309A/L312A construct 5′-GAA​TGA​TGG​CAT​CCG​CCA​CCC​CAC​TTG​CAC​CGC​CAC​AAC​AGC-3′/5′-GCT​GTT​GTG​GCG​GTG​CAA​GTG​GGG​TGG​CGG​ATG​CCA​TCA​TTC-3′ Eurofins 
Human LMX1B Δ308-317 5′-CAG​ATC​GTG​GCC​ATG​GAA​CAG-3′/5′-AGC​CAT​CAT​GCC​CTC​CAT​GC-3′ Eurofins 
Human LMX1B Δ308-317 construct 5′-CAG​ATT​GTG​GCC​ATG​GAG​CAG​TCA​CCG​TA-3′/5′-TGC​CAT​CAT​TCC​CTC​CAT​TCT​AGA​GGA​CAG​G-3′ Eurofins 
Human LMX1AY290A/L293A 5′-GGA​AGG​TAT​CAT​GAA​TCC​GGC​TAC​AGC​GGC​GCC​AAC​ACC​ACA​GCA​GTT​GC-3′/5′-GCA​ACT​GCT​GTG​GTG​TTG​GCG​CCG​CTG​TAG​CCG​GAT​TCA​TGA​TAC​CTT​CC-3′ Eurofins 
Human LC3BF52A/L53A 5′-GTT​CTG​GAT​AAA​ACA​AAG​GCC​GCT​GTA​CCT​GAC​CAT​GTC-3′/5′-GAC​ATG​GTC​AGG​TAC​AGC​GGC​CTT​TGT​TTT​ATC​CAG​AAC-3′ Eurofins 
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