Table 1. Astral microtubule dynamics and... | Rockefeller University Press

Table 1.

Astral microtubule dynamics and soluble tubulin levels in diploid cells

Genotype	Median length (µm)^{^a}	Polym rate (µm/min)^{^a}	Depolym rate (µm/min)^{^a}	Catastrophe frequency (events/min)^{^b}	Rescue frequency (events/min)^{^b}	Dynamicity (subunits/s)^{^b}	α-tubulin molecules per cell^{^c}	β-tubulin molecules per cell^{^c}
Wild type (n = 30 cells)	0.93 (0.91–0.96)	1.27 (1.2–1.41)	1.91 (1.72–2.22)	0.81 ± 0.11	1.02 ± 0.19	0.49 ± 0.03	2.3 × 10⁴ ± 8.8	1.5 × 10⁴ ± 0.3
tub1Δ/TUB1 (n = 20 cells)	0.71 (0.69–0.73)	1.15 (1.02–1.31)	1.60 (1.34–1.84)	0.62 ± 0.12	0.9 ± 0.2	0.43 ± 0.06	1.2 × 10⁴ ± 0.3	2.4 × 10⁴ ± 0.3
tub2Δ/TUB2 (n = 35 cells)	0.73 (0.7–0.76)	1.02 (0.95–1.14)	1.53 (1.42–1.71)	0.8 ± 0.09	0.87 ± 0.12	0.41 ± 0.02	1.5 × 10⁴ ± 0.4	1.1 × 10⁴ ± 0.3
tub1Δ/TUB1 tub2Δ/TUB2 (n = 36 cells)	0.72 (0.7–0.74)	0.97 (0.93–1.1)	1.43 (1.27–1.70)	0.74 ± 0.11	0.88 ± 0.14	0.39 ± 0.04	2.4 × 10⁴ ± 12.3	1.7 × 10⁴ ± 0.6

Genotype	Median length (µm)^{^a}	Polym rate (µm/min)^{^a}	Depolym rate (µm/min)^{^a}	Catastrophe frequency (events/min)^{^b}	Rescue frequency (events/min)^{^b}	Dynamicity (subunits/s)^{^b}	α-tubulin molecules per cell^{^c}	β-tubulin molecules per cell^{^c}
Wild type (n = 30 cells)	0.93 (0.91–0.96)	1.27 (1.2–1.41)	1.91 (1.72–2.22)	0.81 ± 0.11	1.02 ± 0.19	0.49 ± 0.03	2.3 × 10⁴ ± 8.8	1.5 × 10⁴ ± 0.3
tub1Δ/TUB1 (n = 20 cells)	0.71 (0.69–0.73)	1.15 (1.02–1.31)	1.60 (1.34–1.84)	0.62 ± 0.12	0.9 ± 0.2	0.43 ± 0.06	1.2 × 10⁴ ± 0.3	2.4 × 10⁴ ± 0.3
tub2Δ/TUB2 (n = 35 cells)	0.73 (0.7–0.76)	1.02 (0.95–1.14)	1.53 (1.42–1.71)	0.8 ± 0.09	0.87 ± 0.12	0.41 ± 0.02	1.5 × 10⁴ ± 0.4	1.1 × 10⁴ ± 0.3
tub1Δ/TUB1 tub2Δ/TUB2 (n = 36 cells)	0.72 (0.7–0.74)	0.97 (0.93–1.1)	1.43 (1.27–1.70)	0.74 ± 0.11	0.88 ± 0.14	0.39 ± 0.04	2.4 × 10⁴ ± 12.3	1.7 × 10⁴ ± 0.6

Values in bold have P < 0.05 from wild type, based on a t test of P < 0.05 from an ANOVA with a Tukey post-hoc test.

Median values (95% CI).

Mean values ±95% CI.

Mean values ±95% CI; values from Fig. 2 G.