Table 1.

Binding parameters of VASH–SVBP complexes interaction with tyrosinated- and detyrosinated-enriched microtubules in variable ionic strength media

Experiment (medium)Ionic strength (mM)MicrotubuleRecombinant protein (concentration)Residence time τ (s)Binding frequency (min−1.nM−1.µm-1)
A (BRB40, 50 mM KCl) 140 Tyr-MTs V1_FL (50 pM) 0.5 80.8 
  Tyr-MTs deadV1_FL (50 pM) 1.0 132.2 
  Tyr-MTs V2_FL (50 pM) >11 13.0 
  Tyr-MTs deadV2_FL (50 pM) >14.6 13.9 
B (BRB80) 173 Tyr-MTs V1_FL (50 pM) 0.8 51.6 
  Tyr-MTs deadV1_FL (50 pM) 0.9 86.4 
C (BRB80, 50 mM KCl) 223 Tyr-MTs V1_FL (1 nM) 0.2 3.0 
  Tyr-MTs deadV1_FL (1 nM) 0. 2 12.7 
  Tyr-MTs V2_FL (100 pM) 3.9 20.4 
  Tyr-MTs deadV2_FL (100 pM) >7.2 13.5 
D (BRB80, 100 mM KCl) 273 Tyr-MTs V2_FL (50 pM) 0.9 14.9 
  Tyr-MTs deadV2_FL (50 pM) 1.4 19.3 
  deTyr-MTs V2_FL (50 pM) 0.6 13.7 
  deTyr-MTs deadV2_FL (50 pM) 0.7 41.0 
E (BRB40, 50 mM KCl) 140 Tyr-MTs V1_FL (50 pM) 0.8 53.4 
  Tyr-MTs deadV1_FL (50 pM) 1.2 89.0 
  deTyr-MTs V1_FL (50 pM) 0.5 42.3 
  deTyr-MTs deadV1_FL (50 pM) 0.6 66.5 

(A) Related to Fig. 1, D, F, and G. (B) Related to Fig. 2 A. (C) Related to Fig. 2 B. (D) Related to Fig. 2, C, G, and H. (E) related to Fig. 2, E and F. A−E represent experiments performed on different days. At least 15 microtubules were analyzed. See Materials and methods for detailed experimental conditions.

[ViewLarge]
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal